Manchester iGEM 2016

Glucose Oxidase Reaction

Oxidation of glucose to Glucono-δ-lactone and Hydrogen Peroxide using Glucose Oxidase (EC 1.1.3.4)

Chemical Equation

$${Glucose} + {Oxygen} \rightleftharpoons {Glucano-} \delta {-lactone} + {Hydrogen} \ {Peroxide}$$
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Rate Equation

$$\nu = \frac{K_{cat}[GOx] \frac{[Glucose]}{K_{m,Glucose}}\left({ 1- \frac{[Glucose]}{[H_2 O_2][{GDL}]} \frac{1}{K_{eq}}} \right)}{1+\frac{[Glucose]}{K_{m,Glucose}}+\frac{[H_2 O_2]}{K_{m,H_2 O_2}}+\frac{[H_2 O_2][GDL]}{K_{m,GDL}K_{m,H_2 O_2}}+\frac{[GLD]}{K_{m,GDL}}} $$

where:

Symbol	Meaning
$$K_{m,A}$$	Michaelis constant of species A
$$K_{cat}$$	Turnover number
$$[A]$$	Concentration of A
$$ \nu$$	Reaction Rate

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Parameter Sourcing

Kcat and Km parameters were collected from BRENDA

Keq was calculated using the Van’t Hoff equation using the Gibbs free energy sourced from MetaCyc

$$ K_{eq} = e^{\frac{-\Delta G} {RT}}$$
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Parameters with uncertainty

Km

Probability density function for the Km of Glucose Oxidase

Uncertainty in Km is introduced through the range of experimental parameters sourced. Properties of the raw data can be seen in the table below:

Property	Value
Minimum	0.815 mM
Maximum	733 mM
Mean	43.3 mM
Parameters Sourced	88

Kcat

Uncertainty in Kcat is introduced through the range of experimental parameters sourced. Properties of the raw data can be seen in the table below:

Property	Value
Minimum	0.03 s^-1
Maximum	2003 s^-1
Mean	617.3 s^-1
Parameters Sourced	33

Keq

Probability density function for the Keq of Glucose Oxidase

Uncertainty in Keq is introduced using a uniform range of temperature variations (298 K ± 5 K). Properties of this can be seen in the table below:

Property	Value
$$- \Delta G	140.833456736 kJ mol^-1
Minimum Temperature	293 K
Maximum Temperature	303 K

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Team:Manchester/Model/GlucoseOxidaseReaction

Glucose Oxidase Reaction

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