Team:UChicago/Parts

• PROJECT
  • PROJECT OVERVIEW
  • DESIGN
  • RESULTS
• PARTS
  • PARTS OVERVIEW
  • BASIC PARTS
• PRACTICES
  • HUMAN PRACTICES
• TEAM
  • TEAM MEMBERS
  • COLLABORATIONS
• DOCUMENTATION
  • SAFETY
  • NOTEBOOK
• ATTRIBUTIONS
  • ATTRIBUTIONS
  • REFERENCES

We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.

BBa_K1879000

This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al^refReference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. , and we included an RBS sequence upstream of the coding sequence.