Team:NYMU-Taipei/Notebook-Lab Book-pBAR Pmcl1 RFP TtrpC

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8/17

  • pBAR _RFP_TtrpC transformed into competent cells

  • pBAR _RFP_TtrpC containing cultures used in 2 in 1

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    8/18

  • pBAR _RFP_TtrpC extracted from transformed cultures and checked with restriction digestion

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    8/19

  • pBAR_RFP_TtrpC checked with restriction digestion (the incorrect bands appeared in gel electrophoresis results)

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    8/27

  • mRFP1 and TtrpC ran ligation PCR

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    8/28

  • RFP_TtrpC digested for the ligation with pBARGPE1 (the concentration was not high enough)

  • mRFP1 and TtrpC ran ligation PCR (failure, we added adtional Mg2+)

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    8/31

  • RFP_TtrpC digested for the ligation with pBARGPE1 (the band existed on wrong site)

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    9/1

  • RFP_TtrpC digested

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    9/2

  • pBAR_RFP_TtrpC transformed into competent cells

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    9/4

  • pBAR_RFP_TtrpC checked with plasmid PCR

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    9/5

  • pBAR_RFP_TtrpC transformed into competent cells (no E.coli cultures appear on the first plate

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    9/6

  • pBAR _RFP_TtrpC transformed into competent cells

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    9/7

  • pBAR_RFP_TtrpC transformed into competent cells

  • pBAR_RFP_TtrpC containing cultures used in 3 in 1

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    9/8

  • pBAR_RFP_TtrpC checked with plasmid PCR

  • pBAR_RFP_TtrpC checked with restriction digestion

  • pBAR_RFP_TtrpC checked with restriction digestion

  • pBAR_RFP_TtrpC plasmid extraction

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    9/9

  • pBAR_RFP_TtrpC checked with restriction digestion

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    9/10

  • mRFP1 and TtrpC ran ligation PCR

  • RFP_TtrpC amplified the target sequence

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    9/13

  • pBAR_RFP_TtrpC transformed into competent cell

  • RFP_TtrpC PCR amplified the target sequence

  • pBAR_RFP_TtrpC containing cultures used in 3 in 1

  • BAR _RFP_TtrpC checked with restriction digestion

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    9/14

  • pBAR _RFP_TtrpC plasmid extraction

  • RFP_TtrpC digested

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    9/16

  • pBAR _RFP_TtrpC checked with restriction digestion

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    9/17

  • pBAR_RFP_TtrpC checked with restriction digestion

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    9/18

  • Transformed pBAR_RFP_TtrpC into competent cells

  • pBAR_RFP_TtrpC containing cultures used in 3 in 1

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    9/20

  • Extracted plasmid pBAR_RFP_TtrpC

  • pBAR_RFP_TtrpC checked with restriction digestion

  • RFP_TtrpC digestion

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    9/21

  • pBAR_RFP_TtrpC checked with restriction digestion

  • pBAR_RFP_TtrpC containing cultures used in 2 in 1

  • pBARGPE1 and RFP_TtrpC digestion

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    9/22

  • Amplified RFP_TtrpC with KOD polymerase

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    7/31

  • Transformed C120_minimal PgpdA into competent cells

  • C120_minimal PgpdA containing cultures used in 2 in 1

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    7/24

  • pBAR_RFP_TtrpC checked with restriction digestion

  • Extracted plasmid pBAR_RFP_TtrpC

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    9/25

  • Transformed pBAR_RFP_TtrpC into competent cells

  • RFP_TtrpC digestion

  • Amplified RFP-TtrpC with KOD polymerase

  • Amplified RFP_TtrpC with KOD polymerase

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    9/26

  • pBAR_RFP_TtrpC containing cultures used in 2 in 1

  • Extracted plasmid pBAR_RFP_TtrpC

  • pBAR_RFP_TtrpC checked with restriction digestion

  • pBAR_RFP_TtrpC checked with restriction digestion

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    9/28

  • pBAR_RFP_TtrpC checked with plasmid PCR

  • Successfully constructed the plasmid pBAR_RFP_TtrpC

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    10/5

  • pBAR_RFP_TtrpC digestion for the ligation with PMcl1)

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    10/8

  • pBAR _RFP_TtrpC digestion

  • Transformed pBAR_PMcl1_RFP_TtrpC into competent cells

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    10/9

  • pBAR_ PMcl1_RFP_TtrpC containing cultures used in 2 in 1

  • Extracted plasmid pBAR_PMcl1_RFP_TtrpC

  • pBAR_ PMcl1_RFP_TtrpC checked with restriction digestion

  • pBAR_ PMcl1_RFP_TtrpC checked with restriction digestion

  • pBAR_ PMcl1_RFP_TtrpC checked with restriction digestion

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    10/10

  • pBAR_ PMcl1_RFP_TtrpC checked with restriction digestion (failure)

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