Team:William and Mary/Basic Part
Bba_K0026550 is a decoy binding array containing 85 TetO repeat sequences, and was derived from an addgene plasmid from Finney-Manchester et al. (2013) (“Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM). This part can be used to shift the transfer function of an aTC inducible promoter to the left (Figure 1). See decoy binding array page for more information.
Figure 1: Population level FACs data comparing the relative fluorescence of a pTet sfGFP and TetR reporter with and without a tetO binding array. While the data is noisy, it is clear that the inflection point of the circuit with the binding array has shifted to the left as expected. Additionally, both circuits experienced a decrease in fluorescence at higher aTC concentrations, this is likely due to the toxicity of aTC in high concentrations. (See binding array page for more details)
BEFORE:
A basic part is a functional unit of DNA that cannot be subdivided into smaller component parts. BBa_R0051 is an example of a basic part, a promoter regulated by lambda cl.
Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are many opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.
Basic Part
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