Team:William and Mary/Results
Over the summer we successfully characterized each individual component of the Circuit Control Toolbox. Each of these components can be used either individually or in concert to orthogonally modify the output of transfer functions of an arbitrary genetic circuit. We also characterized many existing parts and created a predictive mathematical model of molecular titration. The entirety of the circuit toolbox as well as the parts used to characterized them has been submitted to the registry, on a unified backbone designed for ease of use with Gibson Assembly.
Achievements
* Characterized four orthogonal methods to control transfer functions.
1. The ribozyme RiboJ was used to insulate circuits and characterization from genetic context.
2. A synthetic enhancer system was characterized, allowing for multistate response.
3. The Community Library of RBSs was characterized under a variety of conditions, using RiboJ to ensure that results were generalizable. These RBSs can be used to tune the amplitude of the transfer function of an arbitrary genetic circuit.
4. Molecular titration was characterized using a repeat sequence array, which allows an arbitrary genetic circuit to shift it’s transfer function along the x axis.
* Created a suite of parts that allows for the construction of variable length repeat LacO or TetO arrays.
* Participated in the Interlab Measurement Project.
* Created a mathematical model of molecular titration of arbitrary length repeat arrays.
Results
