Team:Paris Saclay/Notebook/July/25
Contents
Wednesday 20th July
Lab work
Visualization
Low Fidelity Dreamtaq PCR of transformed DH5α with pPS16_002
By Mathilde
A DreamTaq PCR was made with the transformed culture pPS16_002 re-plated the day before. The usual protocol was followeed with Tm at 57°c and 5min for the initial denaturation.
We divided up the PCR mix in 6 PCR tubes and added in each one a different clone from the transformed culture. The picked colonies were re-plated on a petri dish medium LB + Ampicillin (50µg/mL) + xGal/IPTG (1/1000).
Results : PCR products expected were :
| Plasmid | pPS16_002 |
|---|---|
| Bande Size pb | 960 |
