Thaw competent cells (40 to 50µL on ice.
TO BE REMOVED: Cell transformation
Aim: Integrate a plasmid into competent cells
Add 2µL (approximatively 50 to 100ng of plasmid DNA to the cells
Mix gently by pipetting up and down 4 to 5 times.
Incubate on ice for 30 minutes.
Heat shock at 42°C for 30 seconds precisely. (use a water bath or a dry heater)
Incubate on ice for 5 minutes.
Add 250 μl SOC media (without antibiotic), and incubate at 37°C for 1h.
Plate cells on LB+ antibiotic Petri dishes (see Protocol 1 for LB preparation and plates preparation).
Incubate at 37°C overnight and check for colonies.