Protocol 5.0

TO BE REMOVED: Cell transformation

Aim: Integrate a plasmid into competent cells

Thaw competent cells (40 to 50µL on ice.
Add 2µL (approximatively 50 to 100ng of plasmid DNA to the cells
Mix gently by pipetting up and down 4 to 5 times.
Incubate on ice for 30 minutes.
Heat shock at 42°C for 30 seconds precisely. (use a water bath or a dry heater)
Incubate on ice for 5 minutes.
Add 250 μl SOC media (without antibiotic), and incubate at 37°C for 1h.
Plate cells on LB+ antibiotic Petri dishes (see Protocol 1 for LB preparation and plates preparation).
Incubate at 37°C overnight and check for colonies.