Team:Ionis Paris/Notebook

Notebook

Competent cells and DNA transformation efficiency tests

• 05/06/2016: Competent cells: E.Coli DH5⍺
• 06/06/2016: Transformation efficiency test : Competent DH5⍺ cells with pSB1C3-RFP plasmid
• 09/06/2016: Competent cells: E.coli BL21
• 10/06/2016: Transformation efficiency test : Competent BL21 cells with pSB1C3-RFP plasmid
• 23/07/2016 : Competent cells DH5⍺
• 25/07/2016 : BB1/BB2: PCRcol _ Transfo. efficiency test on DH5⍺

Amplification of geneBlocks DNA fragments

• 16/06/2016: PCR on P1
• 28/06/2016 : PCR on P2
• 05/07/2016: PCR on P3

Biosensor construction

• 15/07/2016 : pSB1C3-RFP: Transfo.
• 17/07/2016 : pSB1C3-RFP Mini-preparation
• 20/07/2016 : BB1/BB2/BB3 Digestion & Ligation
• 21/07/2016 : BB1/BB2/BB3 Transformation
• 25/07/2016 : BB1/BB2: PCRcol _ Transfo. efficiency test on DH5⍺
• 26/07/2016 : BB2/BB3: Di/Li _ BB1: Miniprep
• 27/07/2016 : BB12: Di/Li/Tr _ BB2/BB3: Tr
• 28/07/2016 : BB12/BB2/BB3: PCRcol
• 29/07/2016 : BB12/BB2/BB3: Miniprep
• 02/08/2016 : BB123: Di-Li-Tr
• 03/08/2016 : BB123: PCRcol
• 04/08/2016 : BB123: Miniprep
• 05/09/2016 : BB12mut/BB123mut: SDM-Tr
• 06/09/2016 : BB12mut/BB123mut: PCRcol
• 07/09/2016 :Mini prep: on DH5⍺ transformed with BB12mut and BB123mut
• 17/09/2016 :PCR : on PA and PB
• 18/09/2016 : Digestion: PA and PB
• 19/09/2016 : BBA/BBB: Digestion/Ligation
• 20/09/2016 : BBA/BBB: Transformation
• 21/09/2016 : BBA/BBB: PCR Colony
• 22/09/2016 : BBA/BBB: Miniprep
• 17/10/2016 : BB2mut/BBX2: PCR ▪︎ BB2mut/BBX2/BBC4/BBG2: Di-Li-Tr
• 18/10/2016 : BB2mut/BBX2/BBC4/BBG2
• 19/10/2016 : BB2mut/BBC4/BBG2: Miniprep ▪︎ BBX2: Di-Li-Tr

Toluene/Bioluminescence assays

• 29/09/2016: GLuc - Toluene bioluminescence assay
• 18/10/2016: XylR Synthesis Test

CelloCad biobricks construction

• 08/09/2016: C1 and C2 PCR
• 11/09/2016: C1 and C2 PCR
• 12/09/2016: C1 and C2 PCR
• 01/10/2016: Transformation: competent DH5⍺ cells with BBX4, BBC5 and BBC1
• 03/10/2016: BBX4, BBC5 & BBC1 Miniprep
• 04/10/2016: BBC2 digestion, ligation and transformation
• 05/10/2016: BBC2 PCR colony
• 06/10/2016: BBC2 miniprep, BBX5 digestion and BB-B0015 transformation
• 08/10/2016: Experiment title
• 10/10/2016: BBG3: PCR ▪︎ G1: Miniprep ▪︎ BBG3/BBX3/BBX5/pSB1A2-C45: Di-Li-Tr
• 11/10/2016: BBX3, BBX5, pSB1A2-C45 and BBG3 colony PCR
• 12/10/2016: BBX3, BBX5, pSB1A2-C45 and BBG3 miniprep; BBX5, BB-B0015, pSB1A2-C45, pSB1C3, BBA digestions; C45 into pSB1C3, C45 into BBA, B0015 into BBX5 ligations; BBC45, BBC3, BBX1 transformations
• 13/10/2016: BBC45, BBC3 and BBX1 colony PCR
• 14/10/2016: BBC45, BBC3, BBX1 miniprep
• 17/10/2016: BBP2mut/BBX2: PCR ▪︎ BBP2mut/BBX2/BBC4/BBG2: Di-Li-Tr
• 18/10/2016: BB2mut/BBX2/BBC4/BBG2
• 19/10/2016 : BB2mut/BBC4/BBG2: Miniprep ▪︎ BBX2: Di-Li-Tr

Supplementary biobrick construction

• 01/10/2016: Transformation: competent DH5⍺ cells with BBX4, BBC5 and BBC1
• 03/10/2016: BBX4, BBC5 & BBC1 Miniprep
• 06/10/2016: BBC2 miniprep, BBX5 digestion and BB-B0015 transformation
• 07/10/2016: BBX4 miniprep
• 08/10/2016: Experiment title
• 09/10/2016: BBG1 PCR colony
• 10/10/2016: BBG3: PCR ▪︎ G1: Miniprep ▪︎ BBG3/BBX3/BBX5/pSB1A2-C45: Di-Li-Tr
• 11/10/2016: BBX3, BBX5, pSB1A2-C45 and BBG3 colony PCR
• 12/10/2016: BBX3, BBX5, pSB1A2-C45 and BBG3 miniprep; BBX5, BB-B0015, pSB1A2-C45, pSB1C3, BBA digestions; C45 into pSB1C3, C45 into BBA, B0015 into BBX5 ligations; BBC45, BBC3, BBX1 transformations
• 13/10/2016: BBC45, BBC3 and BBX1 colony PCR
• 14/10/2016: BBC45, BBC3, BBX1 miniprep
• 17/10/2016: BBP2mut/BBX2: PCR ▪︎ BBP2mut/BBX2/BBC4/BBG2: Di-Li-Tr
• 18/10/2016: BB2mut/BBX2/BBC4/BBG2
• 19/10/2016 : BB2mut/BBC4/BBG2: Miniprep ▪︎ BBX2: Di-Li-Tr