Team:Ionis Paris/Protocols

Protocols

Protocol List

• Protocol 1 : Culture medium preparation
• Protocol 2 : TAE preparation
• Protocol 3 : Electrophoresis and Gel Purification
• Protocol 4 : Digestion Ligation
• Protocol 5 : Preparation of competent cells
• Protocol 6 : E.Coli dh5a and BL21 transformation via heat shock
• Protocol 7 : PCR and PCR purification
• Protocol 8 : Colony PCR
• Protocol 9 : Miniprep
• Protocol 10 : Site directed mutagenesis
• Protocol 11 : Glycerol Storage
• Protocol 12: Cell Survival assay
• Protocol 13 : Luciferase assay