Tuesday, June 21st
- Grow up DH5α
- Review S. aureus Cas9 sequence
All cells save 2 lines from the previous day did not grow. The most likely reason for the transformation failure is a typo on the iGEM website. The procedure said to resuspend the DNA in 100 uL of water, but the instructions most likely meant 10 uL. We believe the excess DNA over-stressed the competent cells.
Shu & Sara
- Grew 5 more 5 mL LB + DH5a E. coli tubes
- Incubated overnight
- Wrote iGEM preliminary description for the website
Tyler & Sara
- Found the sequence encoding Cas9 in Staphylococcus aureus.