Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 13th October 1.1 Visualization 1.1.1 Plasmids extraction of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October 1.1.2 Digestion of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October Thursday 13th October Visualization Plasmids extraction of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October By Sylvie The following plasmids were extracted using a "standard Plasmid Miniprep": 24 clones containing pPS16_020 (GFP 1.9) pPS16_021 (FRB - GFP 11 FKBP - GFP 10) Digestion of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October Extracted pPS16_020 and pPS16_021 were digested by restriction enzyme NotI: 4 µL of plasmid 2 µL of buffer Tango 1 µL of restriction enzyme NotI 13 µL of water The mix were incubated for 30 minutes at 37°C. Then, 20 µL of digestion products and 15 µL of DNA ladder were placed in wells and migrated at 100V during 30 min. Result of the migration
By Sylvie
The following plasmids were extracted using a "standard Plasmid Miniprep":
Extracted pPS16_020 and pPS16_021 were digested by restriction enzyme NotI:
The mix were incubated for 30 minutes at 37°C. Then, 20 µL of digestion products and 15 µL of DNA ladder were placed in wells and migrated at 100V during 30 min.