Vector-borne (re) emerging diseases are responsible for severe epidemics worldwide. In most cases, vaccines or treatments are not available, and insecticides are the primary source for vector control. Consequently, over-spraying of insecticides impacts the environment and leads to the selection of insecticide-resistant mosquitoes. Therefore, we developed a novel diagnostic device, Mos(kit)o, that includes a fixed or mobile mosquito trap and a biosilica cellulose composite patch from genetically modified E. coli bacteria. The design of the patch creates a multilayered matrix coated with antibodies able to detect a wide panel of vector-borne pathogens and insecticide-resistant proteins from captured mosquitoes. Additionally, the patch will have 2D barcoded readouts, generating an environmental surveillance database. A precise map of vector hot spots will provide a better assessment and response to vector-borne diseases, assisting local health authorities with anticipating and preparing for an epidemic. Our tool will be user-friendly, safe, and applicable.