(→Gel of digested products) |
(→Lab work) |
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= Monday 3<sup>rd</sup> October= | = Monday 3<sup>rd</sup> October= | ||
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===Visualization=== | ===Visualization=== | ||
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− | + | [[File:T--Paris Saclay--Gel11111116.png|400px|thumb|center|Result of the migration]] | |
− | + | [[File:T--Paris Saclay--Gel11111117.png|400px|thumb|center|Result of the migration]] | |
====Digestion of GFP 1.9 in pUC19 (pPS16_009) clone 1==== | ====Digestion of GFP 1.9 in pUC19 (pPS16_009) clone 1==== | ||
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''By Maxence & Caroline'' | ''By Maxence & Caroline'' | ||
− | Extracted pPS16_019 and pPS16_018 from the 2nd October were digested by restriction enzymes EcoRI in order to verify the concentration | + | Extracted pPS16_019 and pPS16_018 from the 2nd October were digested by restriction enzymes EcoRI in order to verify the concentration as following: |
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* 5 µL of plasmid | * 5 µL of plasmid | ||
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* 11 µL of water | * 11 µL of water | ||
− | The mix were incubated for 15 minutes at 37°C. | + | The mix were incubated for 15 minutes at 37°C. |
====Gel of digested products==== | ====Gel of digested products==== | ||
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After digestion, 20 µL of digestion products and 5 µL of DNA ladder were placed in wells and migrated at 100V during 30 min. | After digestion, 20 µL of digestion products and 5 µL of DNA ladder were placed in wells and migrated at 100V during 30 min. | ||
− | + | [[File:T--Paris Saclay--Gel11111118.png|400px|thumb|center|Result of the migration]] | |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} |
Latest revision as of 15:53, 14 October 2016