|
|
| (40 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| − | {{Newcastle}} | + | {{Newcastle |
| − | <br><br>
| + | }} |
| − | <br><br>
| + | |
| − | | + | |
| | <html> | | <html> |
| − | <head>
| |
| − | <link href='https://fonts.googleapis.com/css?family=Kaushan+Script' rel='stylesheet' type='text/css'>
| |
| − | <head>
| |
| − | <script src="https://2016.igem.org/Team:Newcastle/turnjs?action=raw&ctype=text/javascript" type="text/javascript"></script>
| |
| | <style> | | <style> |
| − | body {
| + | #HQ_page th |
| − | background: #F1594B;
| + | |
| − | }
| + | |
| − | #bodyContent{
| + | |
| − | background: #F1594B;
| + | |
| − | }
| + | |
| − | .page
| + | |
| | { | | { |
| − | background-color: #EFE6C5;
| + | background-color: #e35c5c; |
| − | box-shadow: -1px 1px #77aaff,
| + | padding: 10px; |
| − | -2px 2px #77aaff,
| + | color: #292000; |
| − | -3px 3px #77aaff,
| + | border: none; |
| − | -4px 4px #77aaff,
| + | font-size: 2rem; |
| − | -5px 5px #77aaff;
| + | |
| − | border-left: 1px solid #F49D5A;
| + | |
| − | | + | |
| | } | | } |
| − | | + | #HQ_page td |
| − | .page-inner
| + | |
| | { | | { |
| − | padding: 10px, 10px, 10px 10px;
| + | color: #292000; |
| − | margin-left: 12.5px;
| + | border: none; |
| − | margin-right: 12.5px;
| + | font-family: 'Didact Gothic'; |
| − | margin-bottom:20px;
| + | font-size: 2rem; |
| − | margin-top:12.5px;
| + | |
| − | height: 100%;
| + | |
| | } | | } |
| | | | |
| − | .jumbotron
| + | #HQ_page table |
| | { | | { |
| − | padding-top: 80px;
| + | border: none; |
| − | padding-bottom: 0;
| + | |
| | } | | } |
| | | | |
| − | #flipbook
| + | figcaption |
| | { | | { |
| − | width: 980px;
| + | text-align: center; |
| − | height: 600px;
| + | /* font-weight: bold; */ |
| | + | font-size: 1.5rem; |
| | + | color: #292000; |
| | } | | } |
| | | | |
| − | | + | figure img |
| − | #flipbook h1
| + | |
| | { | | { |
| − | font-family: 'Kaushan Script', cursive;
| + | max-width: 100%; |
| − | color: #F1594B;
| + | transform: scale(0.9, 0.9); |
| − | font-size: 2em;
| + | display: block; |
| − | text-align: center;
| + | margin-left: auto; |
| | + | margin-right: auto; |
| | } | | } |
| | | | |
| − | #flipbook h2
| + | a, a:focus, a:hover, li > a |
| | { | | { |
| − | font-family: 'Kaushan Script', cursive;
| + | color: #e35c5c; |
| − | color: #F1594B;
| + | |
| − | | + | |
| − | font-size: 1.25em;
| + | |
| | } | | } |
| − | | + | .col-md-4 a{ |
| − | #flipbook p, #flipbook ul li | + | color: #e35c5c; |
| | + | } |
| | + | .nav > li > a:focus, .nav > li > a:hover, .nav-pills > li.active > a |
| | { | | { |
| − | color: #F1594B;
| + | background-color: #e35c5c; |
| − | font-size: 1em;
| + | |
| | } | | } |
| | | | |
| − | .middle
| + | #hq_page p |
| | { | | { |
| − | margin-top:50%;
| + | font-size: 2rem; |
| | } | | } |
| − | | + | div p, ul li, ol li |
| − | | + | |
| − | .book-wrapper
| + | |
| | { | | { |
| − |
| + | color: #292000; |
| − | padding: 10px 10px 10px 10px;
| + | |
| − | background-color: #F49D5A;
| + | |
| | } | | } |
| | | | |
| − | #scroll-indicator
| + | ul.checked, ul.question, ul.dot |
| | { | | { |
| − | text-align: center;
| + | list-style: none; |
| − | font-size: 4em;
| + | |
| | } | | } |
| | | | |
| − | .navbar-brand | + | ul.checked li, ul.question li, ul.dot li |
| | { | | { |
| − | color: #FFF !important;
| + | font-family: 'Didact Gothic'; |
| − | }
| + | font-size: 16px; |
| − | .logo{
| + | |
| − | margin-top:60px;
| + | |
| − | margin-left:82.5px;
| + | |
| | } | | } |
| | | | |
| − | //----
| + | ul.checked li:before { |
| | + | position: absolute; |
| | + | margin-left: -1.3em; |
| | + | font-weight: bold; |
| | | | |
| − | </style>
| + | content: "\2713"; |
| − | <div class="book-wrapper">
| + | color: darkgreen; |
| − | <div id="flipbook">
| + | } |
| − | <div class="page"><div class="page-inner" style="
| + | |
| − | margin-top: 0px;
| + | |
| − | "><div class="logo"><img alt="Culture Shock"src="https://static.igem.org/mediawiki/2016/6/67/NCLlogo.png" width="300" height="300"></div> | + | |
| − | <h1 style="
| + | |
| − | margin-top: -30px;
| + | |
| − | ">Culture Shock Diary </h1>
| + | |
| − | <p style="text-align:center;">Click on the corner of the page to turn over.</p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1 class="middle"> iGEM Week 1 </h1>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> June 20th, 2016 </h1>
| + | |
| − | <h2> 09:00 - 17:00 </h2>
| + | |
| − | <p> Josh had his training today and was introduced to lab techniques that the rest of the team had worked on during the previous week. These techniques included fire drills, safety techniques as well as use of the sterile hood. </p>
| + | |
| − | <p> The team got split into two groups
| + | |
| − | <ul> <li> Group 1 (Kristina, Ollie and Josh): Made streak plates of all the colonies needed for the Interlab study. These streak plates were left to incubate over the weekend. Alongside colony cultures, liquid cultures were also made. </li> </ul>
| + | |
| − | <ul> <li> Group 1 (Jake, Emilija, Ollie, Kristina, Kerry, Rupert and Lauren): Looked at research papers in order to obtain </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <p> more scientific data for the experiment. We wrote up the agenda as well as the minutes from the meeting we had. We also planned out the rest of the week and began a plan for the Wiki. </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>June 21<sup>st<sup></h1>
| + | |
| − | <p> The morning was spent brainstorming ideas for wiki themes and layout. It was decided to make a baseball themed cards for the team members and old Coca Cola advert was used for a colour scheme and theme. Then we had a lecture about COMSOL and MATLAB by one of our supervisors Dana Ofiteru. In the afernoon we checked the strak plates and then split into sub-groups to research Beyond the bench & Light Bulb component of the breadboard. At the end of the day we skyped with Edinburgh to discuss possible collaboration.
| + | |
| − | </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>June 22<sup>nd<sup></h1>
| + | |
| − | <p> In the morning Jake and Kristina analysed the first Interlab Results while Ollie and Josh researched papers on fuel cells and components for the light bulb. At the same time Emilija and Lauren researched components for the light bulb and tried to construct some BioBricks on Benchling. Rupert designed the Navigation bar for the wiki and Kerry worked on the project description for the Wiki which deadline is on the 1st of July.</p>
| + | |
| | | | |
| − | <p> In the afternoon all of us met with Professor Ian Head, Dr. Ed Milner and Paniz Izadi from the School of Civil Engineering and Geosciences to discuss microbial fuel cells. After that light bulb sub-team met with supervisors to discuss our light bulb biobrick and then we went to a weekly meeting with our supervisors (see Agenda and Minutes for the 22nd June).</p>
| + | ul.question li:before { |
| | + | position: absolute; |
| | + | margin-left: -1.3em; |
| | + | font-weight: bold; |
| | | | |
| − | </div></div>
| + | content: "?"; |
| − | <div class="page"><div class="page-inner"><h1> June 23rd, 2016 </h1>
| + | color: #3c0e09; |
| − | <h2> 09:00 - 11:00 </h2>
| + | } |
| − | <p> <ul> <li> We finalized the Killer BioBrick construct.</li> </ul> </p>
| + | |
| − | <h2> 11:00 - 12:00 </h2>
| + | |
| − | <p> <ul> <li> We had a lecture on modelling by Anil Wipat.</li> </ul> </p>
| + | |
| − | <h2> 12:00 - 12:30 </h2>
| + | |
| − | <p> <ul> <li> Lunch break time!</li> </ul> </p>
| + | |
| − | <h2> 12:30 - 14:00 </h2>
| + | |
| − | <p> <ul> <li> Continued our training in modelling BioBricks after having lunch. We were also introduced to RuleBender. </li> </ul> </p>
| + | |
| − | <h2> 14:00 - 17:00 </h2>
| + | |
| − | <p> <ul> <li> Attempted to model our Killer BioBrick using RuleBender. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> June 24th, 2016 </h1>
| + | |
| − | <h2> 09:30 - 10:30 </h2>
| + | |
| − | <p> <ul> <li> We had a late start due to the outcome of the EU Referendum. As a team bonding activity we all decided to go out for breakfast together before beginning work. </li> </ul> </p>
| + | |
| − | <h2> 10:30 - 11:00 </h2>
| + | |
| − | <p> <ul> <li> We all went to the library and double-checked our BioBrick design that we created using Benchling. Once we had agreed on the design we met up with Jem (one of our advisors) in order to verify that everything was ready to be sent off. </li> </ul> </p>
| + | |
| − | <h2> 11:00 - 12:00 </h2>
| + | |
| − | <p> <ul> <li> Before lunch we went through all of the Action Points. We categorized them into groups based on what was completed and what still needed to be done. The tasks that needed to be done were assigned amongst the team. </li> </ul> </p>
| + | |
| − | <h2> 12:00 - 17:00 </h2>
| + | |
| − | <p> <ul> <li> All the previously assigned tasks were carried out. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1 class="middle"> iGEM Week 2 </h1>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> June 27th, 2016 </h1>
| + | |
| − | <h2> 09:00 - 13:00 </h2>
| + | |
| − | <p> <ul> <li> We visited FabLab in Sunderland. They offer 3D printing, laser printing as well as much more. We were able to tour the lab and get a better idea of what that company could offer us. We decided that the two areas which interested us were the 3D printing for our breadboard but also the formation of electrical circuits. </li> </ul> </p>
| + | |
| − | <h2> 13:00 - 14:00 </h2>
| + | |
| − | <p> <ul> <li> Lunch break time! </li> </ul> </p>
| + | |
| − | <h2> 14:00 - 15:00 </h2>
| + | |
| − | <p> <ul> <li> Meeting with Andrew Filby to be introduced and become accustomed to the concept of flow cytometry. </li> </ul> </p>
| + | |
| − | <h2> 15:00 - 16:00 </h2>
| + | |
| − | <p> <ul> <li> Obtained components for the fuel cell. Components included methylene blue, carbon electrode, cationic exchange membrane alongside other chemicals. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h2> 14:00 - 17:00 </h2>
| + | |
| − | <p> <ul> <li> Went through action points and planned what needed to be completed. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> June 28th, 2016 </h1>
| + | |
| − | <h2> 09:00 - 10:00 </h2>
| + | |
| − | <p> <ul> <li> Made microbial fuel cell. See below for the microbial fuel cell protocol. </li> </ul> </p>
| + | |
| − | <h2> 10:00 - 12:00 </h2>
| + | |
| − | <p> <ul> <li> Liquid cultures of our samples were made and then left in the fridge overnight to be ready for incubation tomorrow. </li> </ul> </p>
| + | |
| − | <h2> 12:00 - 13:00 </h2>
| + | |
| − | <p> <ul> <li> Lunch break time! </li> </ul> </p>
| + | |
| − | <h2> 13:00 - 17:00 </h2>
| + | |
| − | <p> We went to the library where we carried out a variety of tasks </p>
| + | |
| − | <p> <ul> <li> The breadboard design was made using TinkerCad software. We designed an approximation of the sizes of different components as well as how they would fit into the breadboard. </li> </ul> </p>
| + | |
| − | <p> <ul> <li> In the meantime the light-bulb construct was redesigned.</li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <p> <ul> <li> The Team Wiki was looked at and altered. We tried to fix the Navigation Bar and get some information up</li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> June 30th, 2016 </h1>
| + | |
| − | <h2> 09:00 - 11:00 </h2>
| + | |
| − | <p> <ul> <li> The InterLab study was discussed with Tom. We discussed the errors that were made (systematic and experimental). We ran through the steps of the protocol with him and explained what was done at each step. Then we discussed limitations as well as modifications for the experiment. </li> </ul> </p>
| + | |
| − | <h2> 11:00 - 12:00 </h2>
| + | |
| − | <p> <ul> <li> The light-bulb design construct was run through with Jem. </li> </ul> </p>
| + | |
| − | <h2> 12:00 - 13:00 </h2>
| + | |
| − | <p> <ul> <li> Lunch break time! </li> </ul> </p>
| + | |
| − | <p> <ul> <li> The team members who were going to Paris also went into town to buy essentials and exchange Euros. </li> </ul> </p>
| + | |
| − | <h2> 13:00 - 15:00 </h2>
| + | |
| − | <p> <ul> <li> More cultures were grown up for another run of the InterLab study. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <p> <ul> <li> IWBDA was booked and the Paris tickets were confirmed and picked up. </li> </ul> </p>
| + | |
| − | <h2> 15:00 - 17:00 </h2>
| + | |
| − | <p> <ul> <li> Started writing up the final versions of various protocols we were using (for the InterLab, for the microbial fuel cell,etc). </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> July 1st, 2016 </h1>
| + | |
| − | <h2> 12:00 - 21:00 </h2>
| + | |
| − | <p> <ul> <li> Half of the team travelled to Paris while the other half stayed back in Newcastle. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> July 2nd, 2016 </h1>
| + | |
| − | <h2> 09:00 - 12:00 </h2>
| + | |
| − | <p> <ul> <li> Exhibition Stands in Paris. </li> </ul> </p>
| + | |
| − | <h2> 12:00 - 14:00 </h2>
| + | |
| − | <p> <ul> <li> Lunch Break </li> </ul> </p>
| + | |
| − | <h2> 14:00 - 15:30 </h2>
| + | |
| − | <p> <ul> <li> Roundtable 1 - Synthetic Biology: Challenges and Risks
| + | |
| − | <ul><li> François Képès - Research Director at CNRS, founding director of the Epigenomics Project an iSSB </ul></li> <ul><li> Héloïse Muller - PhD at Institute Pasteur </ul></li> <ul><li> Andrew Tolonen - Leader of Tolonen group at the Genoscope-CEA </ul></li> <ul><li> Christophe Genisset - Security Officer at SGDSN (French Secretariat for Defence and National Security) </ul></li> <ul><li> Alexei Grinbaum - Researcher at LARSIM, the Philosophy of Science Group at CEA-Saclay </ul></li></li></ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h2> 15:30 - 16:00 </h2>
| + | |
| − | <p> <ul> <li> Coffee Break </li> </ul> </p>
| + | |
| − | <h2> 16:00 - 17:30 </h2>
| + | |
| − | <p> <ul> <li> Roundtable 2 - Synthetic Biology: A New Economic World <ul><li> Philippe Jais - Chief Executive and Scientific Officer at Eukarys </ul></li> <ul><li> Cyrille Pauthenier -President of Abolis Biotechnologies </ul></li> <ul><li> Samuel Juillot - CSO at Glowee (In absentia - sent a PhD student in his place) </ul></li> <ul><li> Michael Krel - CEO at Enobraq </ul></li> </li> </ul> </p>
| + | |
| − | <h2> 17:30 - 18:00 </h2>
| + | |
| − | <p> <ul> <li> Talk from Randy Rettberg about iGEM and its importance in the world </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> July 3rd, 2016 </h1>
| + | |
| − | <h2> 09:00 - 18:00 </h2>
| + | |
| − | <p> <ul> <li> Site-seeing around Paris </li> </ul> </p>
| + | |
| − | <h2> 18:00 - 02:10 </h2>
| + | |
| − | <p> <ul> <li> Travel back to Newcastle </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1 class="middle"> iGEM Week 3 </h1>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> July 4th, 2016 </h1>
| + | |
| − | <h2> 09:00 - 17:00 </h2>
| + | |
| − | <p> <ul> <li> COMSOL Training Day. The Multiphysics libraries were looked at and the sample projects were completed. Then using the sample as a guideline, we started trying to make a model for our experiment. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> July 5th, 2016 </h1>
| + | |
| − | <h2> 09:00 - 13:00 </h2>
| + | |
| − | <p> <ul> <li> The InterLab study was run again. See below for the protocol. </li> </ul> </p>
| + | |
| − | <h2> 13:00 - 14:00 </h2>
| + | |
| − | <p> <ul> <li> Lunch break time! </li> </ul> </p>
| + | |
| − | <h2> 14:00 - 16:00 </h2>
| + | |
| − | <p> <ul> <li> COMSOL modelling continued. Errors came up with regards to Temperature Coupling so we were working on trying to solve the error. </li> </ul> </p>
| + | |
| − | <p> <ul> <li> In the meantime, the rest of the team was working on the Action Points and trying to complete what hadn't been done yet. </li> </ul> </p>
| + | |
| − | <h2> 16:00 - 17:00 </h2>
| + | |
| − | <p> <ul> <li> Attended a seminar entitled: "Synthetic Biology: Perspectives from artificial life" which was conducted by Professor Martin Hanczyc. His area of expertise revolved around microbial fuel cells and therefore his seminar was of great use. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <p> <ul> <li> He seemed interested in our iGEM project which led to us Skyping him at a further date described later on. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> July 6th, 2016 </h1>
| + | |
| − | <h2> 09:00 - 09:30 </h2>
| + | |
| − | <p> <ul> <li> Checked the plate reader in order to obtain the results from the InterLab. </li> </ul> </p>
| + | |
| − | <h2> 09:30 - 12:00 </h2>
| + | |
| − | <p> <ul> <li> Results from the InterLab were processed and put into an Excel document. In the meantime, other members of the team were completing more of the Action Points which had not been done yet. </li> </ul> </p>
| + | |
| − | <h2> 12:00 - 13:00 </h2>
| + | |
| − | <p> <ul> <li> Lunch break time! </li> </ul> </p>
| + | |
| − | <h2> 13:00 - 14:00 </h2>
| + | |
| − | <p> <ul> <li> Skyped Chennai. We were able to exchange ideas and propose collaboration seen as they needed us to carry out some surveys in order for them to see how their project idea would be perceived in the UK and whether or not it would be accepted. </li> </ul> </p>
| + | |
| − | <h2> 14:00 - 15:00 </h2>
| + | |
| − | <p> <ul> <li> Planned and prepared for the afternoon meeting with our supervisors.</li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <p> <ul> <li> We went over the agenda and made sure that we were fully ready for any questions which were going to be asked. </li> </ul> </p>
| + | |
| − | <h2> 15:00 - 17:00 </h2>
| + | |
| − | <p> <ul> <li> Had meeting with our supervisors. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> July 7th, 2016 </h1>
| + | |
| − | <h2> 09:00 - 12:00 </h2>
| + | |
| − | <p> <ul> <li> Started preparation for plasmids to be sent to DNA 2.0 </li> </ul> </p>
| + | |
| − | <h2> 12:00 - 13:00 </h2>
| + | |
| − | <p> <ul> <li> Lunch break time! </li> </ul> </p>
| + | |
| − | <h2> 13:00 - 14:30 </h2>
| + | |
| − | <p> <ul> <li> Continued preparing cultures for freezing and for the plasmids to be sent to DNA 2.0. </li> </ul> </p>
| + | |
| − | <h2> 14:30 - 15:30 </h2>
| + | |
| − | <p> <ul> <li> Ollie and Lauren filmed for the School of Biology </li> </ul> </p>
| + | |
| − | <h2> 15:30 - 17:00 </h2>
| + | |
| − | <p> <ul> <li> Went over and completed Action Points. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1> July 8th, 2016 </h1>
| + | |
| − | <h2> 09:00 - 12:00 </h2>
| + | |
| − | <p> <ul> <li> Mini-prepped plasmids to be sent to DNA 2.0 </li> </ul> </p>
| + | |
| − | <h2> 12:00 - 13:00 </h2>
| + | |
| − | <p> <ul> <li> Lunch break time! </li> </ul> </p>
| + | |
| − | <h2> 13:00 - 14:00 </h2>
| + | |
| − | <p> <ul> <li> Using spectrophotometry, we measured the absorbance values of the plasmids as well as their concentration and packaged up the sample chosen to be sent to DNA 2.0. </li> </ul> </p>
| + | |
| − | <h2> 14:00 - 16:00 </h2>
| + | |
| − | <p> <ul> <li> Went over and worked on Action Points </li> </ul> </p>
| + | |
| − | <h2> 16:00 - 17:00 </h2>
| + | |
| − | <p> <ul> <li> Meeting with Patrick Degenaar to discuss our experiment and potential limitations which it may have. </li> </ul> </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>July 11<sup>th<sup></h1>
| + | |
| − | <p>Today we designed the cloning strategy for the light bulb construct which is expected to arrive at the end of next week. We also arranged for our light bulb construct to be sent off to DNA 2.0 for synthesis. Once this had been done we worked on developing our bug survival protocol ready for conducting the experiment in the following week. Later, we prepared for this week's meetings by producing detailed plans for each of the sub-teams in the form of gantt charts. To finish off the day we Skyped with experiment.com representative Chistina Tran to discuss crowdfunding for our iGEM project and iGEM challenge.</p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>July 12<sup>th<sup></h1>
| + | |
| − | <p>Today we prepared to compete in the experiment.com iGEM challege, writing up descriptions of our work with the aim of attracting crowdfunding. In tandem with this we polished up our wiki pages, in particular we wrote up biographies for the people who have supported us.</p><p>
| + | |
| − | We also registered, and arranged travel for, the Westminster iGEM meetup in August. Finally, we met with our supervisors to discuss cloning strategy for when we get our construct back from DNA 2.0.</p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>July 13<sup>th<sup></h1>
| + | |
| − | <p>We spent the morning looking for funding opportunities and sent out emails to organisations seeking funding. And prepared for our weekly meeting with our supervisors. We also prepared our constructs containing bicistronic RBS and rpoH gene to be sent to IDT to be synthesised. </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>July 14<sup>th<sup></h1>
| + | |
| − | <p>Today was a day of meetings. First up we skyped with the Edinburgh team to discuss possible collaborations and side projects as well as our experiences on the iGEM project thus far. Following this we skyped with Dr Martin Hanczyc from the University of Trento who had recently given a seminar at the university on improving the output of microbial fuel cells to see if he could provide any advice on improving our fuel cell.</p><p>
| + | |
| − | After a brief lunch break during which we finalized our Boston travel details and sent them off to be booked we met with Prof. Gatehouse. There we discussed ethical issues related with GMOs and our project.</p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>July 15<sup>th<sup></h1>
| + | |
| − | <p>Today was spent running bomb calorimetry experiments in order to determine the specific heat capacity of various media in order to inform our COMSOL modelling.</p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner"><h1 class="middle"> iGEM Week 4 </h1>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>July 20<sup>th<sup></h1>
| + | |
| − | <p>Kristina, Ollie and Josh made streak plates of all the colonies for the interlab, which were left to incubate over the weekend and made liquid cultures for use in the lab.
| + | |
| | | | |
| − | Jake, Emilija, Ollie, Kristina, Kerry, Rupert and Lauren read relevant literature, planned the upcoming week, wrote the agenda plan and typed up the minutes from the previous meeting, as well as starting to plan the Wiki.
| + | ul.dot li:before { |
| | + | position: absolute; |
| | + | margin-left: -1.3em; |
| | + | font-weight: bold; |
| | | | |
| − | Josh was introduced to the training and lab techniques the rest of the group had already received, such as sterile microbe handling in the fume hoods, lab safety and fire drills.
| + | content: "\25A0"; |
| − | </p>
| + | color: #292000; |
| − | </div></div>
| + | } |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>July 21<sup>st<sup></h1>
| + | |
| − | <p>Contact made to secure waste water for the microbial fuel cell from the Cassie Building, responding to Open Lab to confirm our requirements and begin setup procedure for building prototypes of the board components. We also got in touch with the PEALS group (ethics committee) to begin booking a training day for our team. Pouring agar plates in advance of bug survival protocol for Friday 22nd. Delivered another sixth form open day, engagement was very strong on this day in terms of ethical debate.
| + | |
| − | </p>
| + | |
| − | </div></div>
| + | |
| − | <div class="page"><div class="page-inner">
| + | |
| − | <h1>July 25<sup>th<sup></h1>
| + | |
| − | <p>
| + | |
| − | This morning we have spent writing bug survival protocol and preparing TBA, M9 and LB media. In the afternoon, half of the team have met with Natalio Krasnogor to pitch our project to him and the other half received FabLab induction and learned how to laser-cut components.
| + | |
| − | </p>
| + | |
| − | </div></div>
| + | |
| | | | |
| − | <div class="page"><div class="page-inner">
| + | #right-menu |
| − | <h1>July 26<sup>th<sup></h1>
| + | { |
| − | <p>We began the day with half of our team (Josh, Kerry, Kristina and Emilija) going into the lab to carry out a trial run of out E.coli survival after heat shock experiment and hit a few snags. The space required to carry out this experiment is quite large and one sterile hood was not enough. We ran the experiment for only half an hour due to these issues. We began to make improvement for the protocol to ensure the full experiment runs much more smoothly next week. </p>
| + | position: fixed; |
| | + | } |
| | | | |
| − | <p>Other members of the team (Ollie, Jake and Rupert) went to work on profiling the voltage-current response of various media, TAE, TBE, LB and 1M NaCl solution. This required the team to build a testing rig and adapt containers to allow the experiments to take place. After profiling the voltage-current response of the media we investigated the temperature change caused by the Joule heating effect on the material. </p>
| + | </style> |
| − | | + | <div class="column"> |
| − | </div></div>
| + | |
| − | <div class=" page"><div class=" page- inner"> | + | |
| − | <p> | + | <br> |
| − | We discovered that we needed a larger voltage than previously anticipated to produce a localised heating effect. This was because the resistivity of our media were found to be higher than originally anticipated. To counter this we started to investigate ways of increasing the current throughput, e.g. by increasing the conductivity. We also learnt how to make microfluidic devices as these will be easier to induce a heating effect in.</p>
| + | <h2>Important Dates</h2> |
| − |
| + | <br><br> |
| − | <p>The team worked in the computer cluster carrying out a range of tasks such as writing up improvements for the E.coli survival protocol, writing our bios for the experiment website and working on our presentation for the Scottish meetup on Thursday 29th at Edinburgh university. Our presentation is set to be an altered version of that given at the sixth form day on Wednesday and Thursday of last week. The presentation will contain information about our project and where we can see our research and ideas in the coming years. We are also posing some important ethical issues that we have and will be encountering throughout our project. </p> | + | <p>01/05/2016 Team Registration for iGEM deadline</p> |
| − | | + | <p>30/06/2016 InterLab Sign-up Deadline</p> |
| − | </div></div>
| + | <p>01/07/2016 Project Description Deadline</p> |
| − | <div class="page"><div class="page-inner"> | + | <p>02/07/2016 <a href="https://2016.igem.org/Team:Newcastle/Collaborations">European Teams Meet Up in Paris</a></p> |
| − | <h1>July 27<sup>th<sup></h1>
| + | <p>15/07/2016 About our Lab Form Deadline</p> |
| − | | + | <p>15/07/2016 About our Project Form Deadline</p> |
| − | The morning we have spent preparing presentation for the Edinburgh meet up on Thursday 28th of July and preparing for launching our experiment.com fundraiser page. In the afternoon we prepared agenda for the meeting with our supervisors and attended the meeting afterwards (see 27th July meeting agenda and minutes).
| + | <p>28/07/2016 <a href="https://2016.igem.org/Team:Newcastle/Collaborations">Scottish Teams Meet Up in Edinburgh</a></p> |
| − | </p> | + | <p>12/08/2016 Track Selection Deadline – Foundational Advance</p> |
| − | </div></div>
| + | <p>12/08/2016 <a href="https://igem.org/Team.cgi">Title and Abstract Deadline</a></p> |
| − | <div class="page"><div class="page-inner"> | + | <p>17/08/2016 – 18/08/2016 <a href="https://2016.igem.org/Team:Newcastle/Collaborations">UK Teams Meet Up in Westminster, London</a></p> |
| − | <h1>July 28<sup>th<sup></h1>
| + | <p>25/08/2016 <a href="https://2016.igem.org/Team:Newcastle/Collaborations">Edinburgh Undergraduate Team Visits Newcastle</a></p> |
| − | | + | <p>31/08/2016 Giant Jamboree Registration Deadline</p> |
| − | Today we attended iGEM 2016 teams Scottish meet up in Edinburgh where we have spent the day exploring the city, listening to other teams iGEM presentation and sharing ideas with like-minded people (see more in Collabotions:Edinburgh).
| + | <p>02/09/2016 <a href="https://2016.igem.org/Team:Newcastle/InterLab">InterLab Data Deadline</a></p> |
| − | </p> | + | <p>09/09/2016 Team Rosters Deadline</p> |
| − | </div></div>
| + | <p>16/09/2016 <a href="https://2016.igem.org/Team:Newcastle/Notebook/Safety">Final Safety Form Deadline</a></p> |
| − | | + | <p>07/10/2016 Team Banners Deadline</p> |
| − | | + | <p>14/10/2016 <a href="https://igem.org/2016_Judging_Form?id=1895">Judging Form Deadline</a></p> |
| − | </div>
| + | <p>19/10/2016 Wiki Freeze</p> |
| − | </div>
| + | <p>21/10/2016 <a href="http://parts.igem.org/cgi/dna_transfer/batch_list.cgi?group_id=2361">Part Submission Deadline</a></p> |
| − | </div>
| + | <p>27/10/2016 – 31/10/2016 Giant Jamboree 2016 @ Hynes Convention Center, Boston, MA</p> |
| − | </div>
| + | |
| − | <script type="text/javascript"> | + | |
| − | $($("#flipbook").turn());
| + | |
| − | </script> | + | |
| | | | |
| | </body> | | </body> |
| | </html> | | </html> |
