Difference between revisions of "Team:ULV-LC-CV"

 
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<title>ULV iGEM 2016</title>
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<link rel="icon" href="https://static.igem.org/mediawiki/igem.org/9/9c/IGEM_logo_2500x2500.png" type="image/x-icon">
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<h2> Welcome to the University of La Verne's iGEM 2016 wiki! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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display:block;
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
 
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
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    <a href="https://2016.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2015/6/6b/IGEM-animated.gif" alt="iGEM Main Page" style="position: absolute; top: 0px; right: 0px; padding-right: 10px" width="200px"></a>
  
  
  
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<h5> Editing your wiki </h5>
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<header>
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
 
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
<ul>
 
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
<li>Be clear about what you are doing and how you plan to do this.</li>
 
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
 
<li>Have lots of fun! </li>
 
</ul>
 
</div>
 
  
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  <ul class="snip1226">
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      <li class="current"><a href="https://2016.igem.org/Team:ULV-LC-CV/Home" data-hover="HOME">HOME</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Team" data-hover="TEAM">TEAM</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Attributions" data-hover="ATTRIBUTIONS">ATTRIBUTIONS</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Description" data-hover="PROJECT">PROJECT</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Parts" data-hover="PARTS">PARTS</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Model" data-hover="MODEL">MODEL</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Notebook" data-hover="NOTEBOOK">NOTEBOOK</a></li><br>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Collaborations" data-hover="COLLABORATIONS">COLLABORATIONS</a></li>
 +
    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Human_Practices" data-hover="INTEGRATED PRACTICES">INTEGRATED PRACTICES</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/HP/Silver" data-hover="SILVER">SILVER</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Entrepreneurship" data-hover="AWARDS">AWARDS</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Safety" data-hover="SAFETY">SAFETY</a></li>   
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    </ul>
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  </nav></p>
  
<div class="column half_size" >
 
<h5>Inspiration</h5>
 
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
<ul>
 
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
</ul>
 
</div>
 
  
<div class="column half_size" >
 
<h5> Uploading pictures and files </h5>
 
<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
  
<div class="button_click"  onClick=" parent.location= 'https://2016.igem.org/Special:Upload '">   
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UPLOAD FILES
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<h2 id="tst1" style=" float:right; border:3px; border-style:solid; border-color:green; padding: 10px; width:850px;margin:30px;"><b> In vivo production of fatty acid methyl esters in cyanobacteria utilizing the insect methyltransferase, <em>Dm</em>JHAMT </b></h2><br><br><br><br><br><br>
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<img style="float:right" class="sponsors" src="https://scontent-sjc2-1.xx.fbcdn.net/v/t1.0-9/13876427_1073675356013224_7885778739641476181_n.jpg?oh=9a114c6766dba8870d77cfb083403aab&oe=5853E869"width="400px" hspace="30"><br><br><br>
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Biodiesel is mainly composed of fatty acid methyl esters (FAMEs) and is a renewable energy source. Currently FAMEs are synthesized through transesterification of free fatty acids (FFAs) using a methyl donor, such as methanol, along with an alkaline catalyst to speed up the reaction. Both the extraction of FFAs and the chemicals used in this process are expensive. We intend to reduce the production cost of biodiesel by producing FAMEs in vivo using an insect methyltransferase called Drosophila melanogaster Juvenile hormone acid O-methyltransferase (<em>Dm</em>JHAMT) within <em>Synechococcus elongates</em> PCC 7942. <em>Dm</em>JHAMT transfers the methyl groups from endogenous S-adenosyl-L-methionine (SAM) to FFAs therefore synthesizing FAMEs. To minimize extraction costs, we aim to induce self-lysis in <em>Synechococcus</em> at maximum optical densities using quorum sensing. By regulating the production of autoinducers with promoters of various strengths, we plan to tune the optical density at which gene expression is activated.
 +
</p></div><br><br><br><br><br>
  
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<h2  id="tst1" style=" float:left; border:3px; border-style:solid; border-color:green; padding: 10px;width:200px; margin:30px;"><b>Accomplishments</b></h2></b></h2><br><br><br><br><br><br>
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<img style="float:left" class="sponsors" src="https://scontent-sjc2-1.xx.fbcdn.net/v/t1.0-9/13921028_1073675329346560_4308061049291383496_n.jpg?oh=9a2b74d8d3667ff57fb1ecc364d3021a&oe=5851B277"width="400px" hspace="30">
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<p class="align right" style="border:3px; border-style:dashed; border-color:green; padding: 1em; margin:100px;" >University of La Verne iGEM 2015 Team took bronze in first showing at iGEM Competition <img src="https://static.igem.org/mediawiki/2015/6/66/IISER_PUNE_bronze_medal.png"width="70px"<br><br>Visit iGEM2015 wiki here:<a target="_blank" href="https://2015.igem.org/Team:LaVerne-Leos"><img src="https://scontent-sjc2-1.xx.fbcdn.net/v/t1.0-9/13912504_1073675419346551_1832857417709391936_n.jpg?oh=e2d7d9ee01ba62d5b68cb151f7e91a9d&oe=585BFC31"width="30px"></p></div><br>
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<h1 style="border:3px; border-style:double; border-color:black; padding: 10px;width:200px;">ABOUT US</h1></center>
 +
 +
<p>We are an iGEM team from the University of La Verne and La Canada High School. We are an interdisciplinary team with members from biology, math, computer science, art, and library science. We are a community-engaged team that hopes to partner with other high schools, non-profits, and local businesses to create innovative solutions to global issues!</p>
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            <em><strong><h2>Follow Us on Facebook!</h2></strong></em>&nbsp;<a href="https://www.facebook.com/profile.php?id=100009310198035">
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            <em><strong><h2>Contact Information:</h2></strong></em>
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<em><strong>Email Us @ </strong></em><a href="ulvigemteam@gmail.com">ulvigemteam@gmail.com    </a> <br>
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<a target="_blank" href="http://sites.laverne.edu/natural-science/"><br>
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            <em><h2>Natural Science Division Office and Biology Department<br>1950 3rd St.
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            La Verne, CA 91750</h2></em >
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        <img class="sponsors" src="http://www.mathworks.com/images/responsive/global/pic-header-mathworks-logo.png">
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<!--iGem logo-->
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    <a href="https://2016.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2015/6/6b/IGEM-animated.gif" alt="iGEM Main Page" style="position: absolute; top: 0px; right: 0px; padding-right: 10px" width="200px"></a>
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<!--Top navigation-->
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<header>
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  <p><nav>
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  <ul class="snip1226">
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      <li class="current"><a href="https://2016.igem.org/Team:ULV-LC-CV/Home" data-hover="HOME">HOME</a></li>
 +
    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Team" data-hover="TEAM">TEAM</a></li>
 +
    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Attributions" data-hover="ATTRIBUTIONS">ATTRIBUTIONS</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Description" data-hover="PROJECT">PROJECT</a></li>
 +
    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Parts" data-hover="PARTS">PARTS</a></li>
 +
    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Model" data-hover="MODEL">MODEL</a></li>
 +
    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Notebook" data-hover="NOTEBOOK">NOTEBOOK</a></li><br>
 +
    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Collaborations" data-hover="COLLABORATIONS">COLLABORATIONS</a></li>
 +
    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Human_Practices" data-hover="INTEGRATED PRACTICES">INTEGRATED PRACTICES</a></li>
 +
    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/HP/Silver" data-hover="SILVER">SILVER</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Entrepreneurship" data-hover="AWARDS">AWARDS</a></li>
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    <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Safety" data-hover="SAFETY">SAFETY</a></li>   
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    </ul>
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  </nav></p>
 
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Latest revision as of 23:27, 19 October 2016

ULV iGEM 2016 iGEM Main Page

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In vivo production of fatty acid methyl esters in cyanobacteria utilizing the insect methyltransferase, DmJHAMT










Biodiesel is mainly composed of fatty acid methyl esters (FAMEs) and is a renewable energy source. Currently FAMEs are synthesized through transesterification of free fatty acids (FFAs) using a methyl donor, such as methanol, along with an alkaline catalyst to speed up the reaction. Both the extraction of FFAs and the chemicals used in this process are expensive. We intend to reduce the production cost of biodiesel by producing FAMEs in vivo using an insect methyltransferase called Drosophila melanogaster Juvenile hormone acid O-methyltransferase (DmJHAMT) within Synechococcus elongates PCC 7942. DmJHAMT transfers the methyl groups from endogenous S-adenosyl-L-methionine (SAM) to FFAs therefore synthesizing FAMEs. To minimize extraction costs, we aim to induce self-lysis in Synechococcus at maximum optical densities using quorum sensing. By regulating the production of autoinducers with promoters of various strengths, we plan to tune the optical density at which gene expression is activated.






Accomplishments







University of La Verne iGEM 2015 Team took bronze in first showing at iGEM Competition
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