Difference between revisions of "Team:Virginia"

(Undo revision 64542 by Cli2016 (talk))
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{{Virginia/stylesheet}}
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{{Virginia/body}}
 
<html>
 
<html>
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<style type="text/css">
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<style>
  
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/********************************* DEFAULT WIKI SETTINGS  ********************************/
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/*Title of Project*/
 +
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 +
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 +
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 +
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border-radius:5px 5px 0 0;
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#sideMenu, #top_title {display:none;}
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body {background-color:white; }
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text-align:center;
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+
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−
 
+
−
/********************************* MENU ********************************/
+
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+
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/*Navigation menu background*/
+
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#menucontainer {
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−
position:fixed;
+
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z-index:50;
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height:47px;
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    text-align:center;
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−
.sidelogos {
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/*Divs for content blocks*/
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+
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−
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−
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+
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−
    width:auto;
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−
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+
color:#66b3ff;
 +
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−
#igemlogospace {
+
/*The bottom div*/
−
margin-left:10px;
+
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−
background-image:url("https://static.igem.org/mediawiki/2016/8/8a/T--Virginia--igemlogocolorfix.jpeg");
+
position:relative;
−
background-size:40px 40px;
+
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 +
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 +
border-radius:5px;
 +
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−
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#stripe2 {
 
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height:100%;
+
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−
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−
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−
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+
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−
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+
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 +
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−
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+
 
−
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/*Background images for bottom div*/
 +
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 +
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 +
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 +
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 +
background-repeat:no-repeat;
 +
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 +
 
 
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}
  
−
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+
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−
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+
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 +
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}
  
−
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+
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−
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−
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−
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+
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}
  
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}
  
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+
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 +
border-radius:0 5px 5px 0;
 
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}
−
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+
 
−
  width:38px;
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/*Fade in divs*/
−
  margin-right:auto;
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#homepagefadein1 {
 +
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 +
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 +
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 +
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transition:opacity 200ms;
 +
background-color:rgba(0,0,0,0.8);
 
}
 
}
−
#igemlogo{
+
 
−
  width:55px;
+
#fadeintext {
 +
margin:20px;
 +
height:328px;
 +
width:550px;
 +
color:white;
 
}
 
}
−
ul#nav {
+
 
−
    display:block;
+
#homepagefadein1 a, #homepagefadein2 a  {
−
    float:left;
+
color:#66b3ff;
−
    padding:0;
+
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−
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−
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+
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−
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+
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−
    width:890px;
+
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−
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+
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−
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+
−
    font-family: Lato, sans-serif;
+
 
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−
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+
 
−
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+
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−
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−
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−
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+
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+
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−
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+
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 +
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 +
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 +
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 +
background-color:rgba(0,0,0,0.8);
 
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−
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+
 
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−
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−
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−
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+
−
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+
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+
 
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−
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+
 
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 +
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−
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+
 
−
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+
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−
    margin:0px;
+
</head>
−
    width:100%;
+
<body>
−
    height:100%;
+
<div class="maindiv">
−
    text-decoration:none;
+
 
−
    background:transparent;
+
<!-----------------------------------Space reserved for menu------------------------------------>
−
    color:white;
+
<div id="menuspace"></div>
 +
 
 +
<!-----------------------------------Container div---------------------------------------------->
 +
<div class="container">
 +
 
 +
<!---------------------------Top image on page------------------------------------------>
 +
<div class="homeimagearea" style="background-color:#ffa366">
 +
<h1 style="line-height:100px">LEU-LOCK</h1>
 +
</div>
 +
 
 +
 
 +
<!---------------------------Page title area-------------------------------------------->
 +
<!--<div class="pagetitle">
 +
<h1 style="color:#66b3ff">Project Overview</h1>
 +
</div>-->
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<!---------------------------Main content section of page------------------------------->
 +
<div class="content1">
 +
<div id="introvideo">
 +
</div>
 +
 
 +
<div id="homediv2">
 +
<div id="stripe">
 +
<div><h1>WHO ARE WE?</h1></div>
 +
</div>
 +
<div id="homepagefadein1">
 +
<div id="fadeintext">
 +
<p><span class="p">We are a team of undergraduate students from the University of Virginia with a shared interest in synthetic biology.
 +
We hail from five different states, and our majors include biology, biochemistry, chemical engineering, biomedical engineering,
 +
neuroscience, statistics, and nanomedicine engineering.<br>
 +
</span></p>
 +
</div>
 +
<a href="https://2016.igem.org/Team:Virginia/Team" target="_blank">More about the team &rarr;</a>
 +
</div>
 +
</div>
 +
 
 +
<div id="proj">
 +
<div id="back1"></div><div id="back2"></div><div id="back3"></div><div id="back4"></div><div id="back5"></div>
 +
<div id="stripe2">
 +
<div><h1>THE PROJECT</h1></div>
 +
</div>
 +
<div id="homepagefadein2">
 +
<div id="fadeintext2">
 +
<h1>Leu-Lock</h1>
 +
<p><span class="p">We set out to design a novel biocontainment mechanism based upon the modification of the leucyl tRNA synthetase enzyme.
 +
Our system causes the biocontained organism to be dependent upon a synthetic form of leucine for survival -
 +
hence the title of our project. To implement the system in <i>E. coli</i>, CRISPR Cas9 was used to knockout the native
 +
<i>E. coli</i> tRNA synthetase gene, and the modified synthetase gene was inserted into the bacterial cells.
 +
</span></p>
 +
</div>
 +
<a href="https://2016.igem.org/Team:Virginia/Description">Project Overview &rarr;<a/>
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
</div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<script>
 +
 
 +
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 +
document.getElementById("homepagefadein1").style.opacity = "1";
 
}
 
}
−
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+
 
−
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+
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 +
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}
 
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−
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+
 
−
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+
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−
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+
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−
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+
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+
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+
−
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+
−
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+
−
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+
−
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+
 
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−
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+
 
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+
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+
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+
−
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+
−
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+
 
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−
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−
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−
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−
}
 
−
</style>
 
  
−
</head>
+
 
−
<body>
+
 
−
<div style="clear: both;"></div>
+
</script>
−
<div id="menucontainer">
+
</body>
−
<div class="navmenubkg">
+
</html>
−
  <div id="navmenubox">
+
−
    <div id="pblogo" class="sidelogos">
+
−
      <div class="logospace" id="homelogospace">
+
−
      <a href="https://2016.igem.org/Team:Virginia" title="Home Page">
+
−
<span class="imgspan"></span>
+
−
      </a>
+
−
      </div>
+
−
    </div>
+
−
    <ul id="nav">
+
−
      <li> <a href="https://2016.igem.org/Team:Virginia/Description" title="Project Overview">PROJECT</a>
+
−
        <div class="navmenubkg2">    
+
−
          <ul>
+
−
            <li  style="margin-left:3px"> <a href="https://2016.igem.org/Team:Virginia/Description" title="Project Overview">PROJECT OVERVIEW</a></li>
+
−
    <li> <a href="https://2016.igem.org/Team:Virginia/Design" title="Design">DESIGN</a> </li>
+
−
    <li> <a href="https://2016.igem.org/Team:Virginia/Modeling" title="Modeling">MODELING</a> </li>
+
−
            <li> <a href="https://2016.igem.org/Team:Virginia/Experiments" title="Experiments">EXPERIMENTS</a> </li>
+
−
            <li> <a href="https://2016.igem.org/Team:Virginia/Proof" title="Proof of Concept">PROOF OF CONCEPT</a> </li>
+
−
    <li> <a href="https://2016.igem.org/Team:Virginia/Results" title="Results">RESULTS</a> </li>
+
−
  </ul>
+
−
        </div>
+
−
      </li>
+
−
      <li> <a href="https://2016.igem.org/Team:Virginia/Parts" title="Parts">PARTS</a>
+
−
        <div class="navmenubkg2">
+
−
          <ul>
+
−
            <li style="margin-left:86px;"> <a href="https://2016.igem.org/Team:Virginia/Parts" title="Parts Overview">PARTS OVERVIEW</a> </li>
+
−
            <li> <a href="https://2016.igem.org/Team:Virginia/Basic_Part" title="Basic Parts">BASIC PARTS</a></li>
+
−
            <li style="width:168px;"> <a href="https://2016.igem.org/Team:Virginia/Composite_Part" title="Composite Parts">COMPOSITE PARTS</a></li>
+
−
            <li> <a href="https://2016.igem.org/Team:Virginia/Part_Collection" title="Parts Collection">PARTS COLLECTION</a></li>
+
−
  </ul>
+
−
        </div>
+
−
      </li>
+
−
      <li> <a href="https://2016.igem.org/Team:Virginia/Human_Practices" title="Human Practices">PRACTICES</a>
+
−
        <div class="navmenubkg2">
+
−
          <ul>
+
−
            <li style="margin-left:180px"> <a href="https://2016.igem.org/Team:Virginia/Human_Practices" title="Human Practices">HUMAN PRACTICES</a></li>
+
−
    <li> <a href="https://2016.igem.org/Team:Virginia/Integrated_Practices" title="Integrated Practices">INTEGRATED PRACTICES</a></li>
+
−
            <li> <a href="https://2016.igem.org/Team:Virginia/HP/Silver" title="Silver">SILVER</a></li>
+
−
            <li> <a href="https://2016.igem.org/Team:Virginia/HP/Gold" title="Gold">GOLD</a></li>
+
−
            <li> <a href="https://2016.igem.org/Team:Virginia/Engagement" title="Engagement">ENGAGEMENT</a></li>
+
−
  </ul>
+
−
        </div>
+
−
      </li>
+
−
      <li> <a href="https://2016.igem.org/Team:Virginia/Entrepreneurship" title="Awards">AWARDS</a>
+
−
        <div class="navmenubkg2">
+
−
  <ul>
+
−
            <li style="margin-left:150px"> <a href="https://2016.igem.org/Team:Virginia/Awards_Overview" title="Awards Overview">AWARDS OVERVIEW</a> </li>
+
−
            <li> <a href="https://2016.igem.org/Team:Virginia/Entrepreneurship" title="Entrepreneurship">ENTREPRENEURSHIP</a> </li>
+
−
    <li> <a href="https://2016.igem.org/Team:Virginia/Hardware" title="Hardware">HARDWARE</a> </li>
+
−
    <li> <a href="https://2016.igem.org/Team:Virginia/Software" title="Software">SOFTWARE</a> </li>
+
−
    <li> <a href="https://2016.igem.org/Team:Virginia/Measurement" title="Measurement">MEASUREMENT</a> </li>
+
−
    <li> <a href="https://2016.igem.org/Team:Virginia/Model" title="Model">MODEL</a> </li>
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Revision as of 15:02, 5 August 2016

LEU-LOCK

WHO ARE WE?

We are a team of undergraduate students from the University of Virginia with a shared interest in synthetic biology. We hail from five different states, and our majors include biology, biochemistry, chemical engineering, biomedical engineering, neuroscience, statistics, and nanomedicine engineering.

More about the team →

THE PROJECT

Leu-Lock

We set out to design a novel biocontainment mechanism based upon the modification of the leucyl tRNA synthetase enzyme. Our system causes the biocontained organism to be dependent upon a synthetic form of leucine for survival - hence the title of our project. To implement the system in E. coli, CRISPR Cas9 was used to knockout the native E. coli tRNA synthetase gene, and the modified synthetase gene was inserted into the bacterial cells.

Project Overview →