Difference between revisions of "Team:ULV-LC-CV/Model"
| Line 1: | Line 1: | ||
| + | |||
| + | |||
| + | <html> | ||
| + | <head> | ||
<html lang="en"> | <html lang="en"> | ||
<head> | <head> | ||
| Line 14: | Line 18: | ||
orange { padding-bottom: 10px} | orange { padding-bottom: 10px} | ||
footer {background-color: orange; padding-top: 10px; padding-bottom: 20px} | footer {background-color: orange; padding-top: 10px; padding-bottom: 20px} | ||
| − | + | article {float:right} | |
</style> | </style> | ||
<style> | <style> | ||
/* Snippet fopr the navigation area */ | /* Snippet fopr the navigation area */ | ||
@import url(https://fonts.googleapis.com/css?family=Raleway:400,500,800); | @import url(https://fonts.googleapis.com/css?family=Raleway:400,500,800); | ||
| − | .snip1226 { | + | .snip1226 { |
| − | + | font-family: 'Raleway', Arial, sans-serif; | |
text-align: left; | text-align: left; | ||
font-size: 1.0em; | font-size: 1.0em; | ||
| Line 61: | Line 65: | ||
background-color: green; | background-color: green; | ||
} | } | ||
| + | |||
.snip1226 a:after { | .snip1226 a:after { | ||
padding: 0.3em 0; | padding: 0.3em 0; | ||
| Line 74: | Line 79: | ||
transform: translateY(100%); | transform: translateY(100%); | ||
} | } | ||
| − | + | ||
| + | .alignleft { | ||
| + | float: left; | ||
| + | } | ||
| + | .alignright { | ||
| + | float: right; | ||
| + | } | ||
| + | |||
| + | h1, h2, p { padding:0 20px; } | ||
| + | #outer:after | ||
| + | clear:both; | ||
| + | display:block; | ||
| + | height:1%; | ||
| + | content:" "; | ||
| + | } | ||
| + | |||
| + | |||
</style> | </style> | ||
</head> | </head> | ||
| + | |||
| + | |||
| + | |||
<!--iGem logo--> | <!--iGem logo--> | ||
<a href="https://2016.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2015/6/6b/IGEM-animated.gif" alt="iGEM Main Page" style="position: absolute; top: 0px; right: 0px; padding-right: 10px" width="200px"></a> | <a href="https://2016.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2015/6/6b/IGEM-animated.gif" alt="iGEM Main Page" style="position: absolute; top: 0px; right: 0px; padding-right: 10px" width="200px"></a> | ||
<!--Top navigation--> | <!--Top navigation--> | ||
| + | |||
<header> | <header> | ||
| − | + | ||
| + | <p><nav> | ||
<ul class="snip1226"> | <ul class="snip1226"> | ||
<li><a href="https://2016.igem.org/Team:ULV-LC-CV/Home" data-hover="HOME">HOME</a></li> | <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Home" data-hover="HOME">HOME</a></li> | ||
| Line 87: | Line 113: | ||
<li><a href="https://2016.igem.org/Team:ULV-LC-CV/Attributions" data-hover="ATTRIBUTIONS">ATTRIBUTIONS</a></li> | <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Attributions" data-hover="ATTRIBUTIONS">ATTRIBUTIONS</a></li> | ||
<li><a href="https://2016.igem.org/Team:ULV-LC-CV/Description" data-hover="PROJECT">PROJECT</a></li> | <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Description" data-hover="PROJECT">PROJECT</a></li> | ||
| − | <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Parts" data-hover="PARTS">PARTS</a></li> | + | <li class="current"><a href="https://2016.igem.org/Team:ULV-LC-CV/Parts" data-hover="PARTS">PARTS</a></li> |
| − | <li | + | <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Model" data-hover="MODEL">MODEL</a></li> |
<li><a href="https://2016.igem.org/Team:ULV-LC-CV/Notebook" data-hover="NOTEBOOK">NOTEBOOK</a></li><br> | <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Notebook" data-hover="NOTEBOOK">NOTEBOOK</a></li><br> | ||
<li><a href="https://2016.igem.org/Team:ULV-LC-CV/Collaborations" data-hover="COLLABORATIONS">COLLABORATIONS</a></li> | <li><a href="https://2016.igem.org/Team:ULV-LC-CV/Collaborations" data-hover="COLLABORATIONS">COLLABORATIONS</a></li> | ||
| Line 99: | Line 125: | ||
| − | <h2 style="float:left" id="tst1" <b> ♦Enzyme Kinetics with Lauric Acid♦</b></h2><br><br> | + | |
| + | |||
| + | |||
| + | |||
| + | </head> | ||
| + | |||
| + | <head> | ||
| + | <style type="text/css"> | ||
| + | #tst1{color: green;} | ||
| + | </head></style> | ||
| + | |||
| + | |||
| + | <h2 style="float:left" id="tst1" <b> ♦Enzyme Kinetics with Lauric Acid♦</b></h2><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
<img style="float:left" class="sponsors" src=" | <img style="float:left" class="sponsors" src=" | ||
https://lh3.googleusercontent.com/vzFrmlnI1dBo-CBW84xZk-cTaXJt2pRQmnhXl28HxiWBSg30ApUrkU8lc6g1QepLFC3Zyb84BYg167s6BpnBNq6EoTvlYh0yU5ojs6dveGZCQ_aD_BSfcRbP_GjDg75K04AmTHWHKS2NgghXw0MBYMf4KWG94VVaARHAI6ookeAiXWQEMxL3nYfYGZSFM-GtgtXKCT9caEgnJKsMFEOHwxJKZiQqsfFApW4Om7a21RRqvpeWSxDmeEGgY0S2oEO14lbR_b9I2KiNYvGlyHhf-0KnSYAm7TPZjjix5tdXvD8N9YNdDKfJKelRja02CnX0W6ObXy7kwLYTogW4MotlPhJuUONFF0MIQhn1G2AI6rDdgwjmvyJCh4GlDKqYoLsbK5-Q5AsUzeQ8HBLjsKFLGMW2jDSta41QKTSIFoioz-XMFEKBWWTUiS4WU0Bm1kgiQGv-Zt0bB8BrnUTb6CWsyglqeDvjZoFJBgkAaDhHlJHXp9I4gaV8LF89tjWLvHFYCmjuWd0XZ-qVP_8uNPnMb8KhpGApP5Ks6cNZe9JxQNX6LRijHIOwExNT5z3LGceLBMRw058X=w1257-h741-k | https://lh3.googleusercontent.com/vzFrmlnI1dBo-CBW84xZk-cTaXJt2pRQmnhXl28HxiWBSg30ApUrkU8lc6g1QepLFC3Zyb84BYg167s6BpnBNq6EoTvlYh0yU5ojs6dveGZCQ_aD_BSfcRbP_GjDg75K04AmTHWHKS2NgghXw0MBYMf4KWG94VVaARHAI6ookeAiXWQEMxL3nYfYGZSFM-GtgtXKCT9caEgnJKsMFEOHwxJKZiQqsfFApW4Om7a21RRqvpeWSxDmeEGgY0S2oEO14lbR_b9I2KiNYvGlyHhf-0KnSYAm7TPZjjix5tdXvD8N9YNdDKfJKelRja02CnX0W6ObXy7kwLYTogW4MotlPhJuUONFF0MIQhn1G2AI6rDdgwjmvyJCh4GlDKqYoLsbK5-Q5AsUzeQ8HBLjsKFLGMW2jDSta41QKTSIFoioz-XMFEKBWWTUiS4WU0Bm1kgiQGv-Zt0bB8BrnUTb6CWsyglqeDvjZoFJBgkAaDhHlJHXp9I4gaV8LF89tjWLvHFYCmjuWd0XZ-qVP_8uNPnMb8KhpGApP5Ks6cNZe9JxQNX6LRijHIOwExNT5z3LGceLBMRw058X=w1257-h741-k | ||
Revision as of 02:41, 20 October 2016
♦Enzyme Kinetics with Lauric Acid♦
Figure was based off of the Michaelis-Menten enzyme reaction: E + S1 + S2ES1+ES2E+P using the values from Sherkanov et. al paper and the following equations: dx(1)=-k1*x(1)*x(2)+k2*x(3)-k5*x(1)*x(4)+k6*x(6)+k9*x(5); dx(2)=-k1*x(1)*x(2)+k2*x(3)+k8*x(5)-k7*x(6)*x(2); dx(3)=-k3*x(3)*x(4)+k4*x(5)-k2*x(3)+k1*x(1)*x(2); dx(4)=-k5*x(1)*x(4)-k6*x(6)+k4*x(5)-k3*x(3)*x(4); dx(5)=k3*x(3)*x(4)+k7*x(6)*x(2)-(k8+k4+k9)*x(5); dx(6)=-k7*x(6)*x(2)+k8*x(5)-k6*x(6)+k5*x(1)*x(4); dx(7)=k9*x(5); Where k1=ka with a value of 7.7min-1; k2=ka2 with a value of 144 min-1; k3=a*k5 with a value is 7.7min-1; k4=a*k6; k5=kb1 with a value is 7.7min-1; k6=kb2 with a value of 0.103min-1; k7=a*k1; k8=a*k2; k9=k7 with a value of 7.7min-1; x(1)=[DmJHAMT] with a value of 3.32μM; x(2)=[SAM] with a value of 0.05μM; x(3)=[DmJHAMT+SAM]; x(4)=[Lauric acid] with a value of 59μM; x(5)=[DmJHAMT+SAM+Lauric acid]; x(6)=[DmJHAMT+Lauric acid]; x(7)=[FAMEs]; ‘a’ is a constant of 1 since it just represents alpha. The starting concentration of lauric acid (59μM) slowly decreases as time progresses because it converts to FAMEs as it gets methylated by s-adenosyl methionine (SAM) with the help of Drosophila melanogaster juvenile hormone adenyl methyltransferase (DmJHAMT). Because of low intracellular concentrations of SAM (0.05μM) and DmJHAMT (3.32μM), FAME production is not seen on this graph.