Tuesday 2^st August

Lab work

Visualization

DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015

By Charlène, Mathilde, Laetitia, Caroline and Léa

A Low fidelity PCR was carried out following the usual protocol and using the universal puc19 primers. 6 clones for each transformations were tested.

Each clone was re-plated and put at 37°C. We also did a liquid culture for each clone in 3mL of LB and Ampicilin(37°C and 180 rpm).

Sequencing of the gBlock 4.2

By Caroline

The PCR products obtain with Q5 on pPS16_008 were send to sequencing.

Culture of DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006, DH5alp|pPS16_007 and DH5alp|pPS16_008

Bu Caroline

One colonie from each transformation was put into LB + Amp (50µg/mL) and incubated overnight at 37°C and 180rpm.

Interlab Study

Device 1 transformation

By Caroline

The device 1 was transformed in DH5alp following the usual Heat-shock protocol. The transformations were streaked on Petri dishes LB+Amp(50µg/mL)+IPTG(0.1µL/mL)+XGal(0.25µL/mL) and incubated overnight at 37°C.