Revision as of 15:09, 28 July 2016

Thursday 28^th July

By Mathilde and Laetitia

PCR was performed on 6 clones for each plasmid.

Thus, the PCR mix was done for 24 tubes following the usual protocol.

Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG.

PCR was done with a Tm at 57°C.

Each PCR Product was put to migrate on an agarose gel. 10µL of blue ladder was used, and the wells were filled with 1µL of PCR preparations.

PCR products expected were :

Plasmid	pPS16_001	pPS16_002
Band Size (bp)	1007	1007

By Charlène

1 mL of each clone cultured yesterday were put in 500µL of glycerol 60%. They were conserved at -30°C.

@@ Line 15: / Line 15: @@
 Each PCR Product was put to migrate on an agarose gel.
+µL of blue ladder was used, and the wells were filled with 1µL of PCR preparations.
+PCR products expected were :
+{| class="wikitable"
+|-
+!'''Plasmid'''
+!pPS16_001
+!pPS16_002
+|-
+!'''Band Size (bp)'''
+|1007
+|1007
+|}
 ===Biobrick Characterization===