Monday 4^th July

Lab work

Visualization

Digestion of plasmids

By Mathilde and Alice

The following plasmids were digested again with EcoR1 and HindIII:

• 1.1 (from the 6 clones that were selected on 29/06/16)
• 2.1 (from the 6 clones that were selected on 29/06/16)
• 3.1 (from the 6 clones that were selected on 29/06/16)
• 3.2 (from the 6 clones that were selected on 29/06/16)

The mix was incubated at 37°C for 1 hour. An electrophoresis was done (0.8% of agarose).

The DNA concentration was still insufficient meaning the extraction step was not efficient.

Bringing DNA closer

Plasmids extraction

By Naiane and Laetitia

Plasmids from the following strains were extracted with the kit "Charge Switch-Pro Plasmid Miniprep":

• DS-NMCas
• DS-SPCasN
• DS-ST1
• DS-TDCasN

Then plasmids were digested with AvrII increasing the DNA quantity (10µL DNA, 1µL AvrII, 2µL Tango buffer, 7µL water). Digestion products were migrated (5µL digestion, 5µL water, 2µL Template:Font color