Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 30th June 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Extraction of the plasmids containing gBlocks Thursday 30th June Lab work Visualization Extraction of the plasmids containing gBlocks By Alice and Lea DNA from bacteria transformed with plasmids containing gBlocks was extracted following the extraction protocol. For each gblock, DNA of 6 different clones was extracted. At the end, plamids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) to send to sequencing.
By Alice and Lea
DNA from bacteria transformed with plasmids containing gBlocks was extracted following the extraction protocol. For each gblock, DNA of 6 different clones was extracted. At the end, plamids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) to send to sequencing.
