Thursday 13^th October

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Plasmids extraction of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October

By Sylvie

The following plasmids were extracted using a "standard Plasmid Miniprep":

• 24 clones containing pPS16_020 (GFP 1.9)
• pPS16_021 (FRB - GFP 11 FKBP - GFP 10)

Digestion of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October

Extracted pPS16_020 and pPS16_021 were digested by restriction enzyme NotI:

• 4 µL of plasmid
• 2 µL of buffer Tango
• 1 µL of restriction enzyme NotI
• 13 µL of water

The mix were incubated for 30 minutes at 37°C. Then, 20 µL of digestion products and 15 µL of DNA ladder were placed in wells and migrated at 100V during 30 min.

Result of the migration