Difference between revisions of "Team:Paris Saclay/Notebook/October/18"

(Electro transformation of DH5a cells with K2039002)
(Electro transformation of DH5a cells with K2039002)
 
Line 12: Line 12:
 
* 1 µL of K2039002 preparation, no dilution
 
* 1 µL of K2039002 preparation, no dilution
 
* 5 µL of K2039002 preparation, no dilution
 
* 5 µL of K2039002 preparation, no dilution
 +
* 1 control (without DNA)
  
 
Transformed cells were plated on LB solid media with Chloramphenicol and Ampicilin.
 
Transformed cells were plated on LB solid media with Chloramphenicol and Ampicilin.

Latest revision as of 22:58, 19 October 2016

Tuesday 18th October

Visualization

Electro transformation of DH5a cells with K2039002

By Sylvie

4 samples of DH5a cells were transformed using electroporation protocol using:

  • 1µL of K2039002 preparation, diluted 10 times
  • 1 µL of K2039002 preparation, no dilution
  • 5 µL of K2039002 preparation, no dilution
  • 1 control (without DNA)

Transformed cells were plated on LB solid media with Chloramphenicol and Ampicilin.