Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Parts Existing parts used for the project In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators: Name Type Description Designer Length (bp) [http://parts.igem.org/Part:BBa_J23119 BBa_J23119] Basic Constitutive promoter family member iGEM2006_Berkeley 35 [http://parts.igem.org/Part:BBa_B0030 BBa_B0030] Basic RBS.1 (strong) - modified from R. Weiss Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003 15 [http://parts.igem.org/Part:BBa_B0015 BBa_B0015] Basic Double terminator (B0010-B0012) (rrnB T1 - T7Te) Reshma Shetty, 2003 129 Parts designed for the project In order to achieve our project; we designed several basic and composite parts: Name Type Description Length (bp) [http://parts.igem.org/Part:BBa_K2039003 BBa_K2039003] Basic Coding sequences of proteins FKBP and GFP10 528 [http://parts.igem.org/Part:BBa_K2039004 BBa_K2039004] Basic Coding sequences of proteins FRB* and GFP11 498 [http://parts.igem.org/Part:BBa_K2039005 BBa_K2039005] Basic Coding sequences of protein GFP 1.9 633 [http://parts.igem.org/Part:BBa_K2039000 BBa_K2039000] Composite Part expressing the dimerization protein FKBP and GFP10 731 [http://parts.igem.org/Part:BBa_K2039001 BBa_K2039001] Composite Part expressing the dimerization protein FRB* and GFP11 701 [http://parts.igem.org/Part:BBa_K2039006 BBa_K2039006] Composite Part expressing the protein GFP 1.9 836 [http://parts.igem.org/Part:BBa_K2039002 BBa_K2039002] Composite Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11) 1440 [http://parts.igem.org/Part:BBa_K2039007 BBa_K2039007] Composite Dimerization system FRB/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9) 2284
In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:
In order to achieve our project; we designed several basic and composite parts:
