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Bridge:
We created the bridge to make our gene RFC compatible and eligible for the Biobrick standards.
5’ CTAGA AAA AAA AAA AAA AAA CA 3’ 3’ T TTT TTT TTT TTT TTT GTAT 5’ 5’ AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCTAGAAAAAAAAAAAAAAAACATATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3’ |
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NADPH P450 REDUCTASE
AGAGAGCAGCCGGGCTGCCAGCGGTGAGTGCTATCTTTCGCGGCGACGGCGGGGTGGGTG
AGGTCGGGCCCCAAGACTCGGGGTTTGCCGGGCGCCTCAGTTCACCGCGGCCGCCTGGCT CTCTGCGTCGGGGTGGGCCCTCGGGCCTGGGCACTGCGCGTCTGGCCCGACGCTCGGGCG
GAAGGCATGGCTCCCACCCCGGCCCTCCTCCGCGTCGCCCTTCCCCTAGAGCTGATCTCC CGCGGCGGCCTGAGGGTCGACTTTTCCACAGCTTGGGCCGAGAGAGCCCGGCAGGCCCTC
GGCTGAGGGTTAGGGCTCGGTATCTCAGGGCTCGGGGTCCAGTCCCCGCGCCGCCGCCCC CCACGGGCTAAGCCACTTTAACTCTCCGAGCCTCAGTTTCCCCATCTGTACGATGAATGG
GCCTCGGGAGGCAGCGAATTCTGGGGGTACCTAACGTGAGAGGCGCTCGATGCATGCTCA TGGGACCCGAATCTAACCCTGCAGCATCAGTTTCATGATCAACATGGGAGACTCCCACGT
GGACACCAGCTCCACCGTGTCCGAGGCGGTGGCCGAAGAAGTATCTCTTTTCAGCATGAC
GGACATGATTCTGTTTTCGCTCATCGTGGGT
The NADPH P40 Reductase is is needed for the NADPH for the electron transfer in a POR system (that is the c-terminal for the protein sequence) for the expression of P450 cytochrome. The system of electron transfer is NADPH → FAD → FMN → P450 → O2
ATGGCTGACTCCCACGTGGACACCAGCTCCACCGTGTCCGAGGCGGTGGCCGAAGAAGTATCTCTTTTCA
GCATGACGGACATGATTCTGTTTTCGCTCATCGTGGGTCTCCTAACCTACTGGTTCCTCTTCAGAAAGAA
AAAAGAAGAAGTCCCCGAGTTCACCAAAATTCAGACATTGACCTCCTCTGTCAGAGAGAGCAGCTTTGTG
GAAAAGATGAAGAAAACGGGGAGGAACATCATCGTGTTCTACGGCTCCCAGACGGGGAAGGAGT
TTGCCAACCGCCTGTCCAAGGACGCCCACCGCTACGGGATGCGAGGCATGTCAGCGGACCCTGAGGAGTA
TGACCTGGCCGACCTGAGCAGCCTGCCAGAGATCGACAACGCCCTGGTGGTTTTCTGCATGGCCACCTAC
GGTGAGGGAGACCCCACCGACAATGCCCAGGACTTCTACGACTGGGAGACAGACGTGGATCTCT
CTGGGGTCAAGTTCGCGGTGTTTGGTCTTGGGAACAAGACCTACGAGCACTTCAATGCCATGGGCAAGTA
CGTGGACAAGCGGCTGGAGCAGCTCGGCGCCCAGCGCATCTTTGAGCTGGGGTTGGGCGACGACGATGGG
AACTTGGAGGAGGACTTCATCACCTGGCGAGAGCAGTTCTGGCCGGCCGTGTGTGAACACTTTGGGGTGG
AAGCCACTGGCGAGGAGTCCAGCATTCGCCAGTACGAGCTTGTGGTCCACACCGACATAGATGCGGCCAA
GGTGTACATGGGGGAGATGGGCCGGCTGAAGAGCTACGAGAACCAGAAGCCCCCCTTTGATGCCAAGAAT
CCGTTCCTGGTCACCACCAACCGGAAGCTGAACCAGGGAACCGAGCGCCACCTCATGCACCTGG
AATTGGACATCTCGGACTCCAAAATCAGGTATGAATCTGGGGACCACGTGGCTGTGTACCCAGCCAACGA
CTCTGCTCTCGTCAACCAGCTGGGCAAAATCCTGGGTGCCGACCTGGACGTCGTCATGTCCCTGAACAAC
CTGGATGAGGAGTCCAACAAGAAGCACCCATTCCCGTGCCCTACGTCCTACCGCACGGCCCTCACCTACT
ACCTGGACATCACCAACCCGCCGCGTACCAACGTGCTGTACGAGCTGGCGCAGTACGCCTCGGAGCCCTC
GGAGCAGGAGCTGCTGCGCAAGATGGCCTCCTCCTCCGGCGAGGGCAAGGAGCTGTACCTGAGCTGGGTG
GTGGAGGCCCGGAGGCACATCCTGGCCATCGACTGCCCGTCCCTGCGGCCCCCCATCGACCACC
TGTGTGAGCTGCTGCCGCGCGCCCGCTACTACTCCATCGCCTCATCCTCCAAGGTCCACCCCAA
CTCTGTGCACATCTGTGCGGTGGTTGTGGAGTACGAGACCAAGGCCGGCCGCATCAACAAGGGCGTGGCC
ACCAACTGGCTGCGGGCCAAGGAGCCTGCCGGGGAGAACGGCGGCCGTGCGCTGGTGCCCATGTTCGTGC
GCAAGTCCCAGTTCCGCCTGCCCTTCAAGGCCACCACGCCTGTCATCATGGTGGGCCCCGGCACCGGGGT
GGCACCCTTCATAGGCTTCATCCAGGAGCGGGCCTGGCTGCGACAGCAGGGCAAGGAGGTGGGGGAGACG
CTGCTGTACTACGGCTGCCGCCGCTCGGATGAGGACTACCTGTACCGGGAGGAGCTGGCGCAGTTCCACA
GGGACGGTGCGCTCACCCAGCTCAACGTGGCCTTCTCCCGGGAGCAGTCCCACAAGGTCTACGTCCAGCA
CCTGCTAAAGCAAGACCGAGAGCACCTGTGGAAGTTGATCGAAGGCGGTGCCCACATCTACGTCTGTGGG
GATGCACGGAACATGGCCAGGGATGTGCAGAACACCTTCTACGACATCGTGGCTGAGCTCGGGGCCATGG
AGCACGCGCAGGCGGTGGACTACATCAAGAAACTGATGACCAAGGGCCGCTACTCCCTGGACGTGTGGAG
CTAGGGGCCTGCCTGCCCCACCCACCCCACAGACTCCGGCCTGTAATCAGCTCTCCTGGCTCCCTCCCGT
AGTCTCCTGGGTGTGTTTGGCTTGGCCTTGGCATGGGCGCAGGCCCAGTGACAAAGACTCCTCTGGGCCT
GGGGTGCATCCTCCTCAGCCCCCAGGCCAGGTGAGGTCCACCGGCCCCTGGCAGCACAGCCCAGGGCCTG
CATGGGGGCACCGGGCTCCATGCCTCTGGAGCCTCTGGCCCTCGGTGGCTGCACAGAAGGGCTCTTTCTC
TCTGCTGAGCTGGCCCAGCCCCTCCACGTGATTTCCAGTGAGTGTAAATAATTTTAAATAACCTCTGGCC
CTTGGAATAAAGTTCTGTTTTCTGTAAGCTT AAA AAA AAA AAA AAA A
AGAGAGCAGCCGGGCTGCCAGCGGTGAGTGCTATCTTTCGCGGCGACGGCGGGGTGGGTG
AGGTCGGGCCCCAAGACTCGGGGTTTGCCGGGCGCCTCAGTTCACCGCGGCCGCCTGGCT CTCTGCGTCGGGGTGGGCCCTCGGGCCTGGGCACTGCGCGTCTGGCCCGACGCTCGGGCG
GAAGGCATGGCTCCCACCCCGGCCCTCCTCCGCGTCGCCCTTCCCCTAGAGCTGATCTCC CGCGGCGGCCTGAGGGTCGACTTTTCCACAGCTTGGGCCGAGAGAGCCCGGCAGGCCCTC
GGCTGAGGGTTAGGGCTCGGTATCTCAGGGCTCGGGGTCCAGTCCCCGCGCCGCCGCCCC CCACGGGCTAAGCCACTTTAACTCTCCGAGCCTCAGTTTCCCCATCTGTACGATGAATGG
GCCTCGGGAGGCAGCGAATTCTGGGGGTACCTAACGTGAGAGGCGCTCGATGCATGCTCA TGGGACCCGAATCTAACCCTGCAGCATCAGTTTCATGATCAACATGGGAGACTCCCACGT
GGACACCAGCTCCACCGTGTCCGAGGCGGTGGCCGAAGAAGTATCTCTTTTCAGCATGAC
GGACATGATTCTGTTTTCGCTCATCGTGGGT
The NADPH P40 Reductase is is needed for the NADPH for the electron transfer in a POR system (that is the c-terminal for the protein sequence) for the expression of P450 cytochrome. The system of electron transfer is NADPH → FAD → FMN → P450 → O2
ATGGCTGACTCCCACGTGGACACCAGCTCCACCGTGTCCGAGGCGGTGGCCGAAGAAGTATCTCTTTTCA
GCATGACGGACATGATTCTGTTTTCGCTCATCGTGGGTCTCCTAACCTACTGGTTCCTCTTCAGAAAGAA
AAAAGAAGAAGTCCCCGAGTTCACCAAAATTCAGACATTGACCTCCTCTGTCAGAGAGAGCAGCTTTGTG
GAAAAGATGAAGAAAACGGGGAGGAACATCATCGTGTTCTACGGCTCCCAGACGGGGAAGGAGT
TTGCCAACCGCCTGTCCAAGGACGCCCACCGCTACGGGATGCGAGGCATGTCAGCGGACCCTGAGGAGTA
TGACCTGGCCGACCTGAGCAGCCTGCCAGAGATCGACAACGCCCTGGTGGTTTTCTGCATGGCCACCTAC
GGTGAGGGAGACCCCACCGACAATGCCCAGGACTTCTACGACTGGGAGACAGACGTGGATCTCT
CTGGGGTCAAGTTCGCGGTGTTTGGTCTTGGGAACAAGACCTACGAGCACTTCAATGCCATGGGCAAGTA
CGTGGACAAGCGGCTGGAGCAGCTCGGCGCCCAGCGCATCTTTGAGCTGGGGTTGGGCGACGACGATGGG
AACTTGGAGGAGGACTTCATCACCTGGCGAGAGCAGTTCTGGCCGGCCGTGTGTGAACACTTTGGGGTGG
AAGCCACTGGCGAGGAGTCCAGCATTCGCCAGTACGAGCTTGTGGTCCACACCGACATAGATGCGGCCAA
GGTGTACATGGGGGAGATGGGCCGGCTGAAGAGCTACGAGAACCAGAAGCCCCCCTTTGATGCCAAGAAT
CCGTTCCTGGTCACCACCAACCGGAAGCTGAACCAGGGAACCGAGCGCCACCTCATGCACCTGG
AATTGGACATCTCGGACTCCAAAATCAGGTATGAATCTGGGGACCACGTGGCTGTGTACCCAGCCAACGA
CTCTGCTCTCGTCAACCAGCTGGGCAAAATCCTGGGTGCCGACCTGGACGTCGTCATGTCCCTGAACAAC
CTGGATGAGGAGTCCAACAAGAAGCACCCATTCCCGTGCCCTACGTCCTACCGCACGGCCCTCACCTACT
ACCTGGACATCACCAACCCGCCGCGTACCAACGTGCTGTACGAGCTGGCGCAGTACGCCTCGGAGCCCTC
GGAGCAGGAGCTGCTGCGCAAGATGGCCTCCTCCTCCGGCGAGGGCAAGGAGCTGTACCTGAGCTGGGTG
GTGGAGGCCCGGAGGCACATCCTGGCCATCGACTGCCCGTCCCTGCGGCCCCCCATCGACCACC
TGTGTGAGCTGCTGCCGCGCGCCCGCTACTACTCCATCGCCTCATCCTCCAAGGTCCACCCCAA
CTCTGTGCACATCTGTGCGGTGGTTGTGGAGTACGAGACCAAGGCCGGCCGCATCAACAAGGGCGTGGCC
ACCAACTGGCTGCGGGCCAAGGAGCCTGCCGGGGAGAACGGCGGCCGTGCGCTGGTGCCCATGTTCGTGC
GCAAGTCCCAGTTCCGCCTGCCCTTCAAGGCCACCACGCCTGTCATCATGGTGGGCCCCGGCACCGGGGT
GGCACCCTTCATAGGCTTCATCCAGGAGCGGGCCTGGCTGCGACAGCAGGGCAAGGAGGTGGGGGAGACG
CTGCTGTACTACGGCTGCCGCCGCTCGGATGAGGACTACCTGTACCGGGAGGAGCTGGCGCAGTTCCACA
GGGACGGTGCGCTCACCCAGCTCAACGTGGCCTTCTCCCGGGAGCAGTCCCACAAGGTCTACGTCCAGCA
CCTGCTAAAGCAAGACCGAGAGCACCTGTGGAAGTTGATCGAAGGCGGTGCCCACATCTACGTCTGTGGG
GATGCACGGAACATGGCCAGGGATGTGCAGAACACCTTCTACGACATCGTGGCTGAGCTCGGGGCCATGG
AGCACGCGCAGGCGGTGGACTACATCAAGAAACTGATGACCAAGGGCCGCTACTCCCTGGACGTGTGGAG
CTAGGGGCCTGCCTGCCCCACCCACCCCACAGACTCCGGCCTGTAATCAGCTCTCCTGGCTCCCTCCCGT
AGTCTCCTGGGTGTGTTTGGCTTGGCCTTGGCATGGGCGCAGGCCCAGTGACAAAGACTCCTCTGGGCCT
GGGGTGCATCCTCCTCAGCCCCCAGGCCAGGTGAGGTCCACCGGCCCCTGGCAGCACAGCCCAGGGCCTG
CATGGGGGCACCGGGCTCCATGCCTCTGGAGCCTCTGGCCCTCGGTGGCTGCACAGAAGGGCTCTTTCTC
TCTGCTGAGCTGGCCCAGCCCCTCCACGTGATTTCCAGTGAGTGTAAATAATTTTAAATAACCTCTGGCC
CTTGGAATAAAGTTCTGTTTTCTGTAAGCTT AAA AAA AAA AAA AAA A
