Experimental Help to BNDS-China
BNDS-China aims to soften the hard water. To achieve this goal, they decided to synthesize the E. coli backbone with ciaX that enable the bacteria to absorb Ca++. Same with us, they are a first-year high school team. Due to the limitation of their high school lab, we provided them with the access of our lab at Institution of Botany, Chinese Academy of Sciences. We did the PCR amplification of ciaX for them and provided the reagents needed for digestion and joining of DNA, including Phusion DNA polymerase, Taq DNA polymerase, PCR purification kit, gel purification kit, plasmid extraction kit, and restriction enzyme for Xba I, Xho I, EcorI and Pst I. We provided the relevant laboratory apparatus as well. In addition, we guided them through the usage of some of the reagents and instruments and sent them some pBS1c3 parts and pGEX-KG expression vectors for them to later clone the target gene to the expression vector and construct parts.