Team:Chalmers Gothenburg/Experiments/Protocols
NOTEBOOK
Protocols
General protocols
B. subtilis protocols
E. coli protocols
B. licheniformis protocols
S. cerevisiae protocols
- Assembly of promoter study
- Assembly pMel-10 + gRNA for pAQR1 (promoter exchange)
- Assembly pMel-10 + gRNA for pGLN1 (promoter exchange)
- DNA isolation yeast
- Yeast electroporation
- Quick genomic extraction
Synechocystis protocols
- A guide on how to grow Synechocystis
- Assembly of all constructs
- Cultivation and transformation of Synechocystis
- Example of transformation of Synechocystis
- Rapid genomic extraction
Y. lipolytica protocols
- Colony PCR Yarrowia
- Example of Yarrowia construct assembly
- Yarrowia DNA extraction
- Yarrowia DNA extraction 2
