Team:Chalmers Gothenburg/Parts
pAQR1 – BBa_K2030000
The promoter to the AQR1 gene. AQR1 codes for a transporter that enhances secretion of excess amino acids. The transcription of AQR1 is induced by amino acids [1].
The part was taken from the S. cerevisiae genome.
pPCK1 – BBa_K2030001
The promoter to the PCK1 gene. PCK1 codes for phosphoenolpyruvate carboxykinase, an enzyme involved in converting oxaloacetate into phosphoenolpyruvate and carbon dioxide [2]. The promoter pPCK1 has an activity which is induced by acetate, glycerol or ethanol [3].
The part was taken from the S. cerevisiae genome.
Acetate kinase (AckA) – BBa_K2030002
The coding region of the AckA gene. The gene codes for acetate kinase, an enzyme which is part of the Phosphate acetyltransferase-acetate kinase pathway and is responsible for catalyzing the conversion of acetyl-CoA into acetate [4].
The part was taken from the Synechocystis genome.
pPYK2 – BBa_K2030003
The promoter to the PYK2 gene. PYK2 codes for pyruvate kinase, an essential enzyme in the glycolysis that catalyzes the conversion of phosphoenolpyruvate and ADP into pyruvate and ATP [5]. Transcription of PYK2 is repressed by glucose [6].
The part was taken from the S. cerevisiae genome.
pGLN1 – Bba_K2030004
The promoter to the GLN1 gene. GLN1 codes for glutamine synthetase, catalyzing the conversion of glutamate and ammonia into glutamine [7]. This part was not sent into the part registry due to it having illegal restriction sites for biobricks.
The part was taken from S. cerevisiae genome.
References
- [1] Velasco I, Tenreiro S, Calderon IL, André B. Saccharomyces cerevisiae Aqr1 Is an Internal-Membrane Transporter Involved in Excretion of Amino Acids. Eukaryotic Cell. 2004;3(6):1492-503.
- [2] Valdés-Hevia MD, de la Guerra R, Gancedo C. Isolation and characterization of the gene encoding phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae. FEBS Letters. 1989;258(2):313-6.
- [3] Weinhandl K, Winkler M, Glieder A, Camattari A. Carbon source dependent promoters in yeasts. MICROBIAL CELL FACTORIES. 2014;13(1):5.
- [4] Anfelt J, Kaczmarzyk D, Shabestary K, Renberg B, Rockberg J, Nielsen J, et al. Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production. MICROBIAL CELL FACTORIES. 2015;14(1):167.
- [5] Burke RL, Tekamp-Olson P, Najarian R. The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae. Journal of Biological Chemistry. 1983;258(4):2193-201.
- [6] Boles E, Schulte F, Miosga T, Freidel K, Schlüter E, Zimmermann FK, et al. Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate. Journal of Bacteriology. 1997;179(9):2987-93.
- [7] Petrovska I, Nuske E, Munder M, Kulasegaran G, Malinovska L, Kroschwald S, et al. Filament formation by metabolic enzymes is a specific adaptation to an advanced state of cellular starvation. ELIFE. 2014;3(3):e02409-.
