Team:METU HS Ankara/Achievements

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Achievements

Bronze

• Team Wiki: Here’s our wiki page !
• Poster: An informative poster about our project is created for the Giant Jamboree poster session.
• Presentation: Our presentation for the Giant Jamboree presentation session is ready.
• Project Attribution: All of the work done for our project is attributed correctly by team members.
• Registry Part Pages: Part pages on the Registry are created for the parts we’ve made.
• Sample Submission: DNA samples of our new parts are submitted to the Registry.
• Safety Forms: Safety Forms are successfully submitted.
• Judging Form: Judging Form is completed and submitted.
• Required information about anyone who helped us through our project can be found in the Attributions page.
• New BioBrick parts are documented and submitted to the iGEM Registry.

Silver

• We’ve experimentally validated and documented the characterization of BBa_K2052014 in the iGEM Parts Registry.
• We’ve collaborated with over 10 registered iGEM team and helped each other in significant ways. More information can be found in our Collaborations page.
• We’ve identified , investigated and addressed important issues beyond the lab bench by numerous Human Practices we’ve made. More information can be found in our Human Practice page.

Gold

• We’ve improved and expanded on our Human Practice activities. More information can be found in our Integrated Human Practices page.
• *CAD1 (cinnamyl alcohol dehydrogenase) enzyme that we were interested previous year was characterized with Fehling colorimetric assay. This year we wanted to show construct work with pSB1C3 vector on protein gel and we were successful. More information can be found at the part registry, Part K1658000, Cinnamyl alcohol dehydrogenase CAD1
• We’ve demonstrated a functional proof of concept of our project. More information can be found in our Proof of Concept page.
• We’ve created a prototype that shows our project working under real-life conditions.The prototype we designed represents the stages of colon cancer and how our project works during those stages.

PROJECT ACHIEVEMENTS

• We cloned FimH, double terminator and arabinose induced promoter to pSB1C3 backbone and shared the gel images for them. After designing primers, we did PCR to confirm this part.
• We cloned RBS and ButCoAT enzyme (which we obtained from Roseburia intestinalis by doing codon bias to our E. coli) to pSB1C3 backbone and shared the gel images. After designing primers, we did PCR to confirm this part.
• We tried our experiments with expression vector, pet28a, however, after hundreds of trials, we failed to get a successful result. So we decided to change our method and used our parts with pSB1C3 backbone to conduct the experiments. By adding GFP to FimH part and RFP to ButCoAT part we designed two new parts which included GFP and RFP. After completing this constructs, we observed a radiation in green color for FimH and a radiation in red color for ButCoAT. We proved them with our gel images.
• In final stage we did co-culture with cancer cells in which we observed green colored cells around them that indicated binding of our E. coli, then red colors that indicated the production of butyrate.
• CAD1 (cinnamyl alcohol dehydrogenase) enzyme that we were interested previous year was characterized with Fehling colorimetric assay. This year we wanted to show construct work with pSB1C3 vector on protein gel and we were successful.