Team:Missouri Rolla/Attributions

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Attributions and Acknowledgments

Missouri S&T iGEM is entirely student-run as a design team under the Student Design and Experiential Learning Center. All lab work is performed by members trained through our Lab Training Program. Our members chose our project with inspiration from Wagenigen UR 2014 and Toulouse 2014, and our wonderful advisors Dr. Katie Shannon and Dr. David Westenberg are always available for advice.

Our lab received generous financial support from the Biological Sciences, Chemistry, and Chemical Engineering departments at Missouri S&T, and Fred Kielhorn. New England BioLabs and Bio Basic provided us with essential lab supplies, and the free DNA from Integrated DNA Technologies formed an integral part of our project this year.

Barbara and Richard Walberg, Darren and Rhonda Yarbrough, Levi Palmer, Melissa Talbott, and Kapila Shubhender graciously contributed to our crowdfunding campaign.

The Crow Lab at Georgia State University kindly offered to test our project on live P. Destructans.

Kurt Studer created the amazing flying Joe Microbe. Kent Gorday created this wiki, while Kira Buckowing and Kent Gorday trained new lab workers in the Spring and Fall semesters.

Ocimene references:
Beck, Z. Q., Calabria, A. R., Miller, M. C., Vaviline, D. V., Nielsen, A. T. (2013). Increased isoprene production using the archaeal lower mevalonate pathway. US Patent 8361762 B2.
Cornelison, C. T., Keel, M. K., Gabriel, K. T., Barlament, C. K., Tucker, T. A., Pierce, G. E., & Crow, S. A. (2014). A preliminary report on the contact-independent antagonism of Pseudogymnoascus destructans by Rhodococcus rhodochrous strain DAP96253. BMC microbiology, 14(1), 246.
Cornelison, C. T., Gabriel, K. T., Barlament, C., & Crow Jr, S. A. (2014). Inhibition of Pseudogymnoascus destructans growth from conidia and mycelial extension by bacterially produced volatile organic compounds. Mycopathologia, 177(1-2), 1-10.
Hahn, F. M., Hurlburt, A. P., & Poulter, C. D. (1999). Escherichia coli Open Reading Frame 696 Is idi, a Nonessential Gene Encoding Isopentenyl Diphosphate Isomerase. Journal of Bacteriology, 181(15), 4499–4504.
Hoyt, J. R., Cheng, T. L., Langwig, K. E., Hee, M. M., Frick, W. F., & Kilpatrick, A. M. (2015). Bacteria isolated from bats inhibit the growth of Pseudogymnoascus destructans, the causative agent of white-nose syndrome.
Martin, V. J., Pitera, D. J., Withers, S. T., Newman, J. D., & Keasling, J. D. (2003). Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nature biotechnology, 21(7), 796-802.
Matasyoh, L. G., Matasyoh, J. C., Wachira, F. N., Kinyua, M. G., Muigai, A. W. T., & Mukiama, T. K. (2007). Chemical composition and antimicrobial activity of the essential oil of Ocimum gratissimum L. growing in Eastern Kenya. African Journal of Biotechnology, 6(6).
Primak, Y. A., Du, M., Miller, M. C., Wells, D. H., Nielsen, A. T., Weyler, W., & Beck, Z. Q. (2011). Characterization of a feedback-resistant mevalonate kinase from the archaeon Methanosarcina mazei. Applied and environmental microbiology, 77(21), 7772-7778.
Vickers, C. E., Bongers, M., Liu, Q., Delatte, T., & Bouwmeester, H. (2014). Metabolic engineering of volatile isoprenoids in plants and microbes. Plant, cell & environment, 37(8), 1753-1775.
Yoon, S. H., Lee, S. H., Das, A., Ryu, H. K., Jang, H. J., Kim, J. Y., ... & Kim, S. W. (2009). Combinatorial expression of bacterial whole mevalonate pathway for the production of β-carotene in E. coli. Journal of biotechnology, 140(3), 218-226.

Leupeptin references:
Cruz-Morales, P., Martínez-Guerrero, C.E., Morales-Escalante, M.A., Yáñez-Guerra, L.A., Kopp, J.F., Feldmann, J., Ramos-Aboites, H.E. and Barona-Gómez, F., 2015. Recapitulation of the evolution of biosynthetic gene clusters reveals hidden chemical diversity on bacterial genomes. bioRxiv, p.020503.
Hori, M., HEMMI, H., SUZUKAKE, K., HAYASHI, H., UEHARA, Y., TAKEUCHI, T. and UMEZAWA, H., 1978. Biosynthesis of leupeptin. The Journal of antibiotics, 31(1), pp.95-98.
Kim, I.S. and Lee, K.J., 1995. Physiological roles of leupeptin and extracellular proteases in mycelium development of Streptomyces exfoliatus SMF13. Microbiology, 141(4), pp.1017-1025.
O’Donoghue, A.J., Knudsen, G.M., Beekman, C., Perry, J.A., Johnson, A.D., DeRisi, J.L., Craik, C.S. and Bennett, R.J., 2015. Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. Proceedings of the National Academy of Sciences, 112(24), pp.7478-7483.
Simmonds, S., Tatum, E.L. and Fruton, J.S., 1947. The utilization of leucine derivatives by a mutant strain of Escherichia coli. Journal of Biological Chemistry, 170, pp.483-489.
Suzukake, K., Hori, M., Tamemasa, O. and Umezawa, H., 1981. Purification and properties of an enzyme reducing leupeptin acid to leupeptin. Biochimica et Biophysica Acta (BBA)-Enzymology, 661(2), pp.175-181.
Suzukake, K., FUJIYAMA, T., HAYASHI, H., HORI, M. and UMEZAWA, H., 1979. Biosynthesis of leupeptin. II. Purification and properties of leupeptin acid synthetase. The Journal of antibiotics, 32(5), pp.523-530.
Suzukake, K., Hayashi, H., Hori, M. and UMEZAWA, H., 1980. Biosynthesis of leupeptin. III. Isolation and properties of an enzyme synthesizing acetyl-L-leucine. The Journal of antibiotics, 33(8), pp.857-862.
Umezawa, H., Okami, Y. and Hotta, K., 1978. Transfer of the leupeptin-producing ability of the strain, Streptomyces roseus MA839-A1, by conjugation. The Journal of antibiotics, 31(1), pp.99-102.