Team:Newcastle/Notebook/Lab/Lightbulb/Week 4

July 11th 2016

We designed the cloning strategy for the light bulb construct which expected to arrive at the end of the week. We also arranged for our light bulb construct to be sent off to DAN2.0 for synthesis. Once this had been done we worked on developing our bug survival protocol ready for conducting the experiment once our DNA had arrived.

July 12th 2016

We prepared to compete in the experiment.com iGEM challenge writing descriptions of our work with the aim of attracting crowdfunding. We started writing about our construct in order to inform people on what we needing funding on. In the afternoon we met with our supervisors to discuss cloning strategies for when we get our construct back from DNA2.0.

July 13th 2016

We prepared our constructs containing bi-cistronic RBS and rpoH genes to be sent to IDT for synthesis.