Member’s Attributions
LI Cheng mainly focused on the overall operating of the team, meanwhile he took part in the evaluation of protein function. He was also involved in team interaction and academic communication.
YANG Xiaoyu was responsible for the plasmid construction at early stage and then mainly focused on the protein purification. He also participated in secretion effect examination.
BAI Ke was in charge of all experiments related to secretion, including plasmid construction, gene expression and secretory protein examination. He is also one of the speakers who give presentation.
DONG Yiming was responsible for building secretion module, parts and the interlab.
LI Yuexuan was responsible for uranyl adsorption capacity test assay.
YANG Changru was mainly responsible for the test experiment (crosslinking) of protein and modeling, as well as designing some plasmids. He also did some quantitative experiments of uranyl absorption and secretion with the cooperation of teammates.
ZHAO Shijun devoted himself to molecular cloning and part construction.
LYV Nayun took part in the protein purification and the analysis of the questionnaire results.
WANG Dingyu was responsible for protein purification and some art design.
LI Jiamian was the art designer and photographer of our team and he participated in wiki building and PowerPoint design.
WEI Jingyi was responsible for plasmid construction for Secretion Part.
WANG Yuqing was the lab manager and in charge of wiki building, she also participated in human practices, art design and part of the experimental work.
TENG Huaiyuan was in charge of modeling and software design. He studied Flory J.Paul's theory in polymer chemistry and furthermore established the model of molecular weight distribution in our case.
NING Xin was mainly responsible for the molecular cloning of the secretion system, while participating in the measurement of proteins's functions as well.
CHANG Xuyao was in charge of plasmid construction of the Secretion Part.
Acknowledgement
We'd like to thank all those who have helped us over the summer. Without their sincere support, the Uranium Reaper would have not been achieved.
General Support
Prof. OUYANG Qi, Prof. LOU Chunbo, Dr. ZHANG Haoqian, ZHANG Yihao and other teachers helped us during our brainstorming and gave us useful suggestions.
Prof. OUYANG Qi and Prof. LIU Chunli kindly provided the laboratory to us.
ZHANG Yihao gave us meticulous guidance and great help, from the beginning to the end.
Materials Support
Dr. ZHANG Wenbing provided us with the plasmid of 3B (Tri-SpyCatcher flanked by 15X linker).
Prof. ZHOU Lu provided us with Super Uranyl-binding Protein (SUP).
Prof. LIU Chunli allowed us to use his lab to do experiments on uranyl ion. The uranyl nitrate solution and Arsenazo III was provided by Prof. LIU.
Prof. LOU Chunbo provided us with B.subtilis, gene sequence of mSA, and gene sequences of heavy metal ions binding proteins (lead-binding protein, mercury-binding protein and cadmium-binding protein).
Dr. DU Pei provided the beads to us and gave us experimental guidance.
BIT-China provided us with the important Interlab kit.
Experiment Equipment Support
Prof. OUYANG Qi provided the Akta Pure to us for protein purification.
Prof. CHANG Zengyi and Dr. WANG Qingsong provided us with the scanner and helped with the setup and scan of gels.
Prof. LIU Chunli provided us with Geiger counter to test the radiation level in our lab.
Core Facilities at School of Life Sciences, Peking University, assisted us with ITC.
The Testing center of College of Environmental Sciences and Engineering, PKU provided the Aurora M90 mass spectrograph.
Theoretical Guidance
ZHANG Yihao and WANG Yu who is a former member of Peking iGEM 2015, gave us thoughtful suggestions about wiki building.
Dr. YU Daqi helped us a lot in experimental operating and data analysis.
Prof. ZHANG Wenbing, Prof. LOU Chunbo and Dr. YU Daqi kindly helped our team with modeling of the project.
Prof. OUYANG Qi, Prof. LOU Chunbo, Dr. ZHANG Haoqian and ZHANG Yihao coached us for the presentation.
LI Ying and ZHANG Xueping provided us with kind suggestions for our presentation.
Prof. LI Chenjian, Prof. TANG Chao, Prof. CHEN Xing, Prof. CHEN Peng and Prof. LAI Luhua participated in our presentation rehearsal and gave suggestions for revisions.
Yiwen Expert Text Solutions (yiwen.ba) provided expert text correction service for the content of our wiki.
NWPU offered us helpful suggestions of PowerPoint.
An Introduction to Synthetic Biology Course
From March to June, an introductory course of synthetic biology was hold by College of Life Sciences, Peking University. During the course, instructors gave us detailed guidance to our original project design, and helped us to make improvement.
