Team:SDU-Denmark/Attributions

Attributions

Laboratory, Technical and General support


Thanks to our supervisors:

  • PhD student and former iGEM participant Thøger Jensen Krogh for guidance in the laboratory and with solving issues with the wiki page.
  • PhD student and former iGEM participant Patrick Rosendahl Andreassen for guidance in the laboratory.
  • Assistant professor Mikkel Girke Jørgensen for advice on team dynamics, keeping us focussed, never giving up on us or the project, and guidance in general about the issues that might come.


And thanks to:

  • Academic Assistant Tina Kronborg has helped us with general tips, ordering everything from enzymes to cars for expeditions. Thank you Tina.
  • Cand. Scient. Andreas Kjær for providing us with general tips, understanding and help in the laboratory.
  • Associate professor Jakob Møller-Jensen for providing with general tips and laboratory help.
  • Medical Laboratory Technician,Simon Rose for providing us with a security course and providing help and tips in the lab.
  • Business Scout and PhD Ann Zahle for providing us with teambuilding exercises and information about a potential patent application.
  • Kemotechnician Anita Lunding - For introducing us to the rotary evaporator
  • Academic Assistant Eva Christina Østergaard for helping us with general usage of different equipment in the lab and general tips to work in the lab.
  • Special consultant Anders Boysen for providing us with general tips in the lab.
  • Cand. Scient. Maj Bay - for assisting with the rotary evaporator.
  • Associate professor Morten Østergaard Andersen for assistance with 3D printing, applications in the field of biomaterial and general tips in the usage and advantages of PHB.
  • Professor in orthopaedic surgery at SDU Ming Ding for having a great talk with us about PHB, its therapeutic applications and the possible futures for PHB.
  • Project Director of Wound Care Innovation of Coloplast Maria Yoland Andersen for giving us a statement from Coloplast about our project.
  • Associate professor Paul C. Stein for assistance with HNMR analysis for detection of PHB.
  • Mast.Stud. Casper Slots and Martin Bonde Jensen for guidance with 3D printing in general, creating our 3D models and tips on printing with our own produced PHB.
  • Assistent professor Yaakov Chaikin from Johns Hopkins University for the great course "HTML, CSS, and Javascript for Web Developers" on coursera.org it was very helpful while developing the wiki.
  • Postdoc in philosophy and biology Sara Green from the University of Copenhagen and PhD in social science and philosophy Søren Engelsen from SDU for sparring and general advices to our philosopher, Rikke Bentzon.
  • Postdoc PhD Arkadiusz Nawrocki for helping us doing GC-MS/MS, MALD-MS and general iTRAQ guidance.
  • PhD Mogens T. Jensen for assistance with gas chromatography.
  • Lab technician of BMB Lykke Haastrup Hansen with general help in the lab.
  • Bach.Stud.Scient. Sarah Hyllekvist Jørgensen for last minute help in the lab.
  • Nurse Laura Jakobsen for participating in an interview. (See interview with Laura Jakobsen)
  • Professor Dr.Med. and project leader Hans Jørn Kolmos for participating in an interview which gave us several new perspectives in the use of Bacteriocins. (See interview with Hans Jørn Kolmos)
  • Professor and head of a research group for genomic epidemiology Frank Møller Aarestrup for a statement on the usage of Bacteriocins.
  • The high schools we visited and presented our iGEM project. This includes Kold Kollege, Odense Technical Gymnasium and Academy for Talented Youth which is a national program for selected students in high school.
  • DTU iGEM team – for arranging the annual biobrick workshop for the Danish teams.
  • The iGEM Marburg team - for having us at the German iGEM meetup in August, and thanks for arranging a great weekend.
  • The iGEM Stockholm team for hosting the annual nordic meetup in June.
  • The Cosmocrops from KU - for arranging a new meetup about human practice in August.
  • The previous SDU iGEMers provided us constructive criticism for our project.
  • The NordCEE group for borrowing one of their incubators to scale up the PHB production.
  • All the companies who answered our survey and gave us constructive feedback: Agstrup Plast ApS, AMU SYD, Fast Plast A/S, Bogense Plast A/S, DAMVIG Develop A/S, HN Group A/S, Letbæk Plast A/S, Malte Haaning Plastic A/S, Martin Høft A/S, MiM Plast A/S, Ossi Connectors A/S, RPC Superfos A/S, Schoeller-Plast-Enterprise A/S and SP Medical A/S.
  • Torben, Per and Klaus from the "workshop of BMB" for making our filament maker "Filastruder" work and finding a large incubator for scale up production of our PHB.
  • Pia Ernlund Drangsfeldt for lending us her cottage where we had our first brainstorm meeting and became friends.


Sponsors


We would like to give a special thanks to our sponsors, who financially supported throughout the project.


  • University of Southern Denmark has given us the funds for the project, which has made it possible for us to participate in iGEM. Here also a special thanks to the faculties at SDU for supporting our project and letting us use their different equipments in the laboratories.
  • Integrated DNA Technologies has provided us with 20 kbp worth of gBlock gene fragments.
  • Eurofins Genomics has provided us with half a mix2seq kit and 75 % discount on our sequencing.
  • LGC Biosearch Technologies has giving us a 30 % discount on their oligos.
  • Pentabase has giving us a sponsorship for purchase of oligos and a further 10 % discount.
  • SnapGene has provided us with free use of their program during the iGEM period.
  • Albani has provided us with t-shirts.
  • Merck has provided us with us with LB broth, taq polymerase and deoxynucleotides.
  • ZIGNS has helped design the wikipage layout and provided graphics.


The whole idea of our product Bacto-Aid as a bandage, was an idea we all contributed to. The lab work diverged into three groups: Plastic, Silk, and Bacteriocins. We wanted to take advantages of the many Biomedicines in our group and therefore each group had a biomedicin as squad leader.

The Plastic group was Birka Jensen, Jakob Rønning and squad leader Joel Vej-Nielsen. Birka helped in the lab until July where her focus switched to programming our wiki-site. Birka literally wrote everything from the bottom.

The Silk group consisted of Mathilde Nygaard, Nete Bækgaard, Viktor Mebus, Rikke Bentzon and squad leader Rune Øbo. We all tried really hard with the lab work, but we did not manage to produce silk fibers, unfortunately. Rikke switched her focus to the philosophic part of our project in august. Rikke is head writer on the Studies on the Precautionary Principle, Theory and Bioethics and Philosophy of science. 

The Bacteriocin group was Brian Baltzar, Astrid Honoré, Pernille Rasmussen and squad leader Cathrine Christensen. They all went crazy in the laboratory and got awesome results!

Beside the three main groups our project diverged into more branches including Funding, Clothes, Graphical design, Integrated human practice, Presentation etc. It was therefore necessary to assign people to different tasks. To sum-up, we all contributed to the project with our individual forces and thus made it a fantastic experience.



Literature


  • Acuña, Leonardo, Gianluca Picariello, Fernan- do Sesma, Roberto D Morero og Augusto Bellomio (2012). “A new hybrid bacteriocin, Ent35–MccV, displays antimicrobial activi- ty against pathogenic Gram-positive and Gram-negative bacteria”. I: FEBS Open Bio 2, s. 12–19. doi: 10.1016/j.fob.2012. 01.002. - Link

  • AGNARSSON, I., BOUTRY, C., WONG, S. C., BAJI, A., DHINOJWALA, A., SENSENIG, A. T. & BLACKLEDGE, T. A. 2009. Supercontraction forces in spider dragline silk depend on hydration rate. Zoology (Jena), 112, 325-31. - Link

  • Alam, M. T., et al. (2015). "Transmission and microevolution of USA300 MRSA in U.S. households: evidence from whole-genome sequencing." MBio 6(2): e00054. - Link 

  • Albertson, A., Teulé, F., Weber, W., Yarger, J. and Lewis, R. (2014). Effects of different post-spin stretching conditions on the mechanical properties of synthetic spider silk fibers. Journal of the Mechanical Behavior of Biomedical Materials, 29, pp.225-234. - Link

  • ALTMAN, G. H., DIAZ, F., JAKUBA, C., CALABRO, T., HORAN, R. L., CHEN, J., LU, H., RICHMOND, J. & KAPLAN, D. L. 2003. Silk-based biomaterials. Biomaterials, 24, 401-416. - Link

  • Andersen, M., Lichawska, A., Arpanaei, A., Rask Jensen, S., Kaur, H., Oupicky, D., Besenbacher, F., Kingshott, P., Kjems, J. and Howard, K. (2010). Surface functionalisation of PLGA nanoparticles for gene silencing. Biomaterials, 31(21), pp.5671-5677. - Link

  • ARAMVASH, A., GHOLAMI-BANADKUKI, N., MOAZZENI-ZAVAREH, F. & HAJIZADEH-TURCHI, S. 2015. An Environmentally Friendly and Efficient Method for Extraction of PHB Biopolymer with Non-Halogenated Solvents. Journal of Microbiology and Biotechnology, 25, 1936-1943. - Link 

  • ARCIOLA, C. R., VISAI, L., TESTONI, F., ARCIOLA, S., CAMPOCCIA, D., SPEZIALE, P. & MONTANARO, L. 2011. Concise survey of Staphylococcus aureus virulence factors that promote adhesion and damage to peri-implant tissues. The International journal of artificial organs, 34, 771-780. - Link 

  • Balciunas, E. M., Castillo Martinez, F. A., Todorov, S. D., Gombossy De Melo Franco, B. D., Converti, A., & Pinheiro De Souza Oliveira, R. (2013). Novel biotechnological applications of bacteriocins: A review. Food Control, 32, 134–142. - Link

  • Baoyong, Lu, Zheng Jian, Chen Denglong og Li Min (2010). “Evaluation of a new type of wound dressing made from recombinant spider silk protein using rat models.” eng. I: Burns 36.6, s. 891–896. issn: 1879-1409 (Electronic); 0305-4179 (Linking). doi: 10. 1016/j.burns.2009.12.001. - Link 

  • BARNES, David KA, et al. Accumulation and fragmentation of plastic debris in global environments. Philosophical Transactions of the Royal Society B: Biological Sciences, 2009, 364.1526: 1985-1998. - Link 

  • Bastos, Maria do Carmo de Freire, Bruna Gon ̧calves Coutinho og Marcus L ́ıvio Va- rella Coelho (2010). “Lysostaphin: A Stap- hylococcal Bacteriolysin with Potential Cli- nical Applications”. I: Pharmaceuticals 3.4, s. 1139–1161. doi: 10 . 3390 / ph3041139. url: - Link

  • BOWLER, P. G., DUERDEN, B. I. & ARMSTRONG, D. G. 2001. Wound microbiology and associated approaches to wound management. Clinical Microbiology Reviews, 14, 244-+. - Link 

  • Ceotto, Hilana, Janaina dos Santos Nascimen- to, Maria Aparecida Vasconcelos de Paiva Brito og Maria do Carmo de Freire Bastos (2009). “Bacteriocin production by Staphy- lococcus aureus involved in bovine masti- tis in Brazil.” eng. I: Res Microbiol 160.8, s. 592–599. issn: 1769-7123 (Electronic); 0923-2508 (Linking). doi: 10 . 1016 / j . resmic.2009.07.007. - Link 

  • Challis, Gregory L (2006). “Engineering Esche- richia coli to produce nonribosomal peptide antibiotics”. I: Nat Chem Biol 2.8, s. 398– 400. - Link  

  • Chehimi, S., Pons, A.-M., Sablé, S., Hajlaoui, M.-R., & Limam, F. (2010). Mode of action of thuricin S, a new class IId bacteriocin from Bacillus thuringiensis. Canadian Journal of Microbiology, 56(2), 162–167. - Link

  • CHISHOLM, A. & CLARKE, H. 1993. Fair principles for sustainable development. Natural Resource Management and the Precautionary Principle (ed. Edward Dommen), Edward Elgar Publishing, 109-122. - Link 

  • COLE, Matthew, et al. The impact of polystyrene microplastics on feeding, function and fecundity in the marine copepod Calanus helgolandicus.Environmental science & technology, 2015, 49.2: 1130-1137. - Link 

  • Conly, J. M., & Johnston, B. L. (2002). VISA, hetero-VISA and VRSA: The end of the vancomycin era? The Canadian Journal of Infectious Diseases, 13(5), 282–284. - Link 

  • Connaughton, A., Childs, A., Dylewski, S. and Sabesan, V.J. (2014) ‘Biofilm disrupting technology for orthopedic Implants: What’s on the horizon?’, 1 - Link

  • Cotter, Paul D, R Paul Ross og Colin Hill (2013). “Bacteriocins - a viable alternative to antibiotics?” eng. I: Nat Rev Microbiol 11.2, s. 95–105. issn: 1740-1534 (Electro- nic); 1740-1526 (Linking). doi: 10 . 1038 / nrmicro2937. - Link 

  • Dams-Kozlowska, H., Majer, A., Tomasiewicz, P., Lozinska, J., Kaplan, D. and Mackiewicz, A. (2012). Purification and cytotoxicity of tag-free bioengineered spider silk proteins. Journal of Biomedical Materials Research Part A, 101A(2), pp.456-464. - Link

  • DAW, M. A. & FALKINER, F. R. 1996. Bacteriocins: nature, function and structure. Micron, 27, 467-79. - Link

  • DOBSON, A., COTTER, P. D., ROSS, R. P. & HILL, C. 2012. Bacteriocin production: a probiotic trait? Appl Environ Microbiol, 78, 1-6. - Link

  • Fredriksson, C., Hedhammar, M., Feinstein, R., Nordling, K., Kratz, G., Johansson, J., . . . Rising, A. (2009). Tissue Response to Subcutaneously Implanted Recombinant Spider Silk: An in Vivo Study. Materials, 2(4), 1908. - Link.

  • GHOSH, S. K., PAL, S. & RAY, S. 2013. Study of microbes having potentiality for biodegradation of plastics. Environmental Science and Pollution Research, 20, 4339-4355. - Link

  • GIL, E. S., PANILAITIS, B., BELLAS, E. & KAPLAN, D. L. 2013. Functionalized silk biomaterials for wound healing. Adv Healthc Mater, 2, 206-17. - Link

  • Gomes, S., Gallego-Llamas, J., Leonor, I., Mano, J., Reis, R. and Kaplan, D. (2011). Biological responses to spider silk-antibiotic fusion protein. Journal of Tissue Engineering and Regenerative Medicine, 6(5), pp.356-368. - Link

  • Gomes, S., Leonor, I., Mano, J., Reis, R. and Kaplan, D. (2011). Antimicrobial functionalized genetically engineered spider silk. Biomaterials, 32(18), pp.4255-4266. - Link

  • Gomes, Silvia C, Isabel B Leonor, Joao F Mano, Rui L Reis og David L Kaplan (2011). “Antimicrobial functionalized gene- tically engineered spider silk.” eng. I: Bio- materials 32.18, s. 4255–4266. issn: 1878- 5905 (Electronic); 0142-9612. - Link

  • HAHN, S. K., CHANG, Y. K. & LEE, S. Y. 1995. Recovery and Characterization of Poly(3-Hydroxybutyric Acid) Synthesized in Alcaligenes-Eutrophus and Recombinant Escherichia-Coli. Applied and Environmental Microbiology, 61, 34-39. - Link

  • HANISCH, J., WALTERRNANN, M., ROBENEK, H. & STEINBUCHEL, A. 2006. The Ralstonia eutropha H16 phasin PhaP1 is targeted to intracellular triacylglycerol inclusions in Rhodococcus opacus PD630 and Mycobacterium smegmatis mc(2)155, and provides an anchor to target other proteins. Microbiology-Sgm, 152, 3271-3280. - Link

  • HARTZELL-NICHOLS, L. 2012. Precaution and solar radiation management. Ethics, Policy & Environment, 15, 158-171. - Link 

  • HEINRICH, D., MADKOUR, M. H., AL-GHAMDI, M. A., SHABBAJ, I. I. & STEINBUCHEL, A. 2012. Large scale extraction of poly(3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite. Amb Express, 2. - Link

  • HERMAN-BAUSIER, P., EL-KIRAT-CHATEL, S., FOSTER, T. J., GEOGHEGAN, J. A. & DUFRÊNE, Y. F. 2015. Staphylococcus aureus fibronectin-binding protein A mediates cell-cell adhesion through low-affinity homophilic bonds. MBio, 6, e00413-15. - Link 

  • HOSEINABADI, A., RASOOLI, I. & TARAN, M. 2015. Isolation and Identification of Poly beta-Hydroxybutyrate Over-Producing Bacteria and Optimization of Production Medium. Jundishapur Journal of Microbiology, 8. - Link

  • INGHAM, Aaron, et al. The bacteriocin piscicolin 126 retains antilisterial activity in vivo. Journal of Antimicrobial Chemotherapy, 2003, 51.6: 1365-1371. - Link

  • JACQUEL, N., LO, C. W., WEI, Y. H., WU, H. S. & WANG, S. S. 2008. Isolation and purification of bacterial poly (3-hydroxyalkanoates). Biochemical Engineering Journal, 39, 15-27. - Link

  • JAMBUNATHAN, P. & ZHANG, K. C. 2016. Engineered biosynthesis of biodegradable polymers. Journal of Industrial Microbiology & Biotechnology, 43, 1037-1058. - Link

  • KAPOOR, S., GUPTA, D., KUMAR, M., SHARMA, S., GUPTA, A. K., MISRO, M. M. & SINGH, H. 2016. Intracellular delivery of peptide cargos using polyhydroxybutyrate based biodegradable nanoparticles: Studies on antitumor efficacy of BCL-2 converting peptide, NuBCP-9. International Journal of Pharmaceutics, 511, 876-889. - Link

  • KAPOOR, S., GUPTA, D., KUMAR, M., SHARMA, S., GUPTA, A. K., MISRO, M. M. & SINGH, H. 2016. Intracellular delivery of peptide cargos using polyhydroxybutyrate based biodegradable nanoparticles: Studies on antitumor efficacy of BCL-2 converting peptide, NuBCP-9. Int J Pharm, 511, 876-89. - Link

  • KARAHALILOGLU, Z., ERCAN, B., TAYLOR, E. N., CHUNG, S., DENKBAS, E. B. & WEBSTER, T. J. 2015. Antibacterial Nanostructured Polyhydroxybutyrate Membranes for Guided Bone Regeneration. J Biomed Nanotechnol, 11, 2253-63. - Link

  • KARAHALILOGLU, Z., ERCAN, B., TAYLOR, E. N., CHUNG, S., DENKBAS, E. B. & WEBSTER, T. J. 2015. Antibacterial Nanostructured Polyhydroxybutyrate Membranes for Guided Bone Regeneration. J Biomed Nanotechnol, 11, 2253-63. - Link

  • Keller, R., Pedroso, M. Z., Ritchmann, R., & Silva, R. M. (1998). Occurrence of Virulence-Associated Properties in Enterobacter cloacae. Infection and Immunity, 66(2), 645–649. - Link

  • KESHAVARZ, T. & ROY, I. 2010. Polyhydroxyalkanoates: bioplastics with a green agenda. Current Opinion in Microbiology, 13, 321-326. - Link

  • Kim, U., Park, J., Joo Kim, H., Wada, M. and Kaplan, D. (2005). Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin. Biomaterials, 26(15), pp.2775-2785. - Link

  • Kluge, J., Rabotyagova, O., Leisk, G. and Kaplan, D. (2008). Spider silks and their applications. Trends in Biotechnology, 26(5), pp.244-251. - Link

  • LEONARDI, R., CHOHNAN, S., ZHANG, Y. M., VIRGA, K. G., LEE, R. E., ROCK, C. O. & JACKOWSKI, S. 2005. A pantothenate kinase from Staphylococcus aureus refractory to feedback regulation by coenzyme A. Journal of Biological Chemistry, 280, 3314-3322. - Link

  • LI, G., LI, Y., CHEN, G., HE, J., HAN, Y., WANG, X. & KAPLAN, D. L. 2015. Silk-based biomaterials in biomedical textiles and fiber-based implants. Adv Healthc Mater, 4, 1134-51. - Link

  • Li, Y., Kumar, K., Dabkowski, J., Corrigan, M., Scott, R., Nüsslein, K. and Tew, G. (2012) New bactericidal surgical suture coating, Langmuir: The ACS journal of surfaces and colloids., 28(33), pp. 12134–9 - Link

  • LIBOIRON, M. 2016. Redefining pollution and action: The matter of plastics. Journal of Material Culture, 21, 87-110. - Link

  • Ling, H., Saeidi, N., Haji Rasouliha, B., & Wook Chang, M. (2010). A predicted S-type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage. FEBS Letters, 584, 3354–3358. - Link

  • Lohans, C. T., & Vederas, J. C. (2012). Development of Class IIa Bacteriocins as Therapeutic Agents. International Journal of Microbiology, 2012, 13. - Link.

  • LUEF, K. P., STELZER, F. & WIESBROCK, F. 2015. Poly(hydroxy alkanoate)s in Medical Applications. Chemical and Biochemical Engineering Quarterly, 29, 287-297. - Link

  • Ma, Q., Yu, Z., Han, B., Wang, Q., & Zhang, R. (2012). Expression and Purification of Lacticin Q by Small Ubiquitin-Related Modifier Fusion in Escherichia coli. The Journal of Microbiology, 50(2), 326–331. - Link

  • MAHISHI, L. H., TRIPATHI, G. & RAWAL, S. K. 2003. Poly(3-hydroxybutyrate) (PHB) synthesis by recombinant Escherichia coli harbouring Streptomyces aureofaciens PHB biosynthesis genes: Effect of various carbon and nitrogen sources. Microbiological Research, 158, 19-27. - Link

  • MEISCHEL, M., EICHLER, J., MARTINELLI, E., KARR, U., WEIGEL, J., SCHMOLLER, G., TSCHEGG, E. K., FISCHERAUER, S., WEINBERG, A. M. & STANZL-TSCHEGG, S. E. 2016. Adhesive strength of bone-implant interfaces and in-vivo degradation of PHB composites for load-bearing applications. J Mech Behav Biomed Mater, 53, 104-18. - Link

  • MEISCHEL, M., EICHLER, J., MARTINELLI, E., KARR, U., WEIGEL, J., SCHMOLLER, G., TSCHEGG, E. K., FISCHERAUER, S., WEINBERG, A. M. & STANZL-TSCHEGG, S. E. 2016. Adhesive strength of bone-implant interfaces and in-vivo degradation of PHB composites for load-bearing applications. J Mech Behav Biomed Mater, 53, 104-18. -Link

  • Nazarov, R., Jin, H. and Kaplan, D. (2004). Porous 3-D Scaffolds from Regenerated Silk Fibroin. Biomacromolecules, 5(3), pp.718-726. - Link

  • Nygaard, J., Andersen, M., Howard, K., Foss, M., Bünger, C., Kjems, J. and Besenbacher, F. (2008). Investigation of particle-functionalized tissue engineering scaffolds using X-ray tomographic microscopy. Biotechnology and Bioengineering, 100(4), pp.820-829. - Link

  • OU, G., BAO, C., LIANG, X., CHAO, Y. & CHEN, Z. 2000. [Histological study on the polyhydroxybutyric ester(PHB) membrane used for guided bone regeneration around titanium dental implants]. Hua Xi Kou Qiang Yi Xue Za Zhi, 18, 215-8. - Link

  • OU, G., BAO, C., LIANG, X., CHAO, Y. & CHEN, Z. 2000. [Histological study on the polyhydroxybutyric ester(PHB) membrane used for guided bone regeneration around titanium dental implants]. Hua Xi Kou Qiang Yi Xue Za Zhi, 18, 215-8. - Link

  • PACHEKOSKI, W. M., AGNELLI, J. A. M. & BELEM, L. P. 2009. Thermal, Mechanical and Morphological Properties of Poly (Hydroxybutyrate) and Polypropylene Blends After Processing. Materials Research-Ibero-American Journal of Materials, 12, 159-164. - Link

  • PADOL, A. R., JAYAKUMAR, K., SHRIDHAR, N. B., NARAYANA SWAMY, H. D., NARAYANA SWAMY, M. & MOHAN, K. 2011. Safety evaluation of silk protein film (a novel wound healing agent) in terms of acute dermal toxicity, acute dermal irritation and skin sensitization. Toxicol Int, 18, 17-21. - Link

  • PETERSON, J. A. & CARTER. M. 2015. On the Epistemology of the Precautionary Principle. Erkenntnis, 80, 1-13. - Link 

  • PORRAS, M. A., CUBITTO, M. A. & VILLAR, M. A. 2016. A new way of quantifying the production of poly(hydroxyalkanoate)s using FTIR. Journal of Chemical Technology and Biotechnology, 91, 1240-1249. - Link

  • RAHMAN, A., LINTON, E., HATCH, A. D., SIMS, R. C. & MILLER, C. D. 2013. Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach. Journal of Biological Engineering, 7. - Link

  • RAMSAY, J. A., BERGER, E., VOYER, R., CHAVARIE, C. & RAMSAY, B. A. 1994. Extraction of Poly-3-Hydroxybutyrate Using Chlorinated Solvents. Biotechnology Techniques, 8, 589-594. - Link

  • ROCHMAN, C. M. 2016. Strategies for reducing ocean plastic debris should be diverse and guided by science. Environmental Research Letters, 11. (Access by SDU) - Link

  • ROCHMAN, C. M., COOK, A. M. & KOELMANS, A. A. 2016. Plastic debris and policy: Using current scientific understanding to invoke positive change. Environmental Toxicology and Chemistry, 35, 1617-1626. - Link

  • RODRIGUEZ-CONTRERAS, A., MARQUES-CALVO, M. S., GIL, F. J. & MANERO, J. M. 2016. Modification of titanium surfaces by adding antibiotic-loaded PHB spheres and PEG for biomedical applications. J Mater Sci Mater Med, 27, 124. - Link

  • RODRIGUEZ-CONTRERAS, A., MARQUES-CALVO, M. S., GIL, F. J. & MANERO, J. M. 2016. Modification of titanium surfaces by adding antibiotic-loaded PHB spheres and PEG for biomedical applications. J Mater Sci Mater Med, 27, 124. - Link

  • Römer, L. and Scheibel, T. (2008). The elaborate structure of spider silk. Prion, 2(4), pp.154-161. - Link

  • SCHMIDT, M. & DE LORENZO, V. 2012. Synthetic constructs in/for the environment: Managing the interplay between natural and engineered Biology. Febs Letters, 586, 2199-2206. - Link

  • SCHUBERT, C., VAN LANGEVELD, M. C. & DONOSO, L. A. 2014. Innovations in 3D printing: a 3D overview from optics to organs. British Journal of Ophthalmology, 98, 159-161. - Link

  • SCHUBERT, P., STEINBUCHEL, A. & SCHLEGEL, H. G. 1988. Cloning of the Alcaligenes-Eutrophus Genes for Synthesis of Poly-Beta-Hydroxybutyric Acid (Phb) and Synthesis of Phb in Escherichia-Coli. Journal of Bacteriology, 170, 5837-5847. - Link

  • SHETH, R., BALESH, E. R., ZHANG, Y. S., HIRSCH, J. A., KHADEMHOSSEINI, A. & OKLU, R. 2016. Three-Dimensional Printing: An Enabling Technology for IR. Journal of Vascular and Interventional Radiology, 27, 859-865. - Link

  • Singh, P. K., Chittpurna, A., Sharma, V., Patil, P. B., & Korpole, S. (2012). Identification, Purification and Characterization of Laterosporulin, a Novel Bacteriocin Produced by Brevibacillus sp. Strain GI-9. - Link

  • Singh, P. K., Solanki, V., Sharma, S., Thakur, K. G., Krishnan, B., & Korpole, S. (2015). The intramolecular disulfide-stapled structure of laterosporulin, a class IId bacteriocin, conceals a human defensin-like structural module. FEBS Journal, 282(2), 203–214. - Link

  • Szweda, P P (08.2001). “Cloning, expression, and purification of the Staphylococcus si- mulans lysostaphin using the intein-chitin- binding domain (CBD) system.” I: Protein expression and purification 22.3, s. 467–471. doi: 10.1006/prep.2001.1454. - Link 

  • Teulé, F., Cooper, A., Furin, W., Bittencourt, D., Rech, E., Brooks, A. and Lewis, R. (2009). A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning. Nature Protocols, 4(3), pp.341-355. - Link

  • TOKAREVA, Olena, et al. Recombinant DNA production of spider silk proteins.Microbial biotechnology, 2013, 6.6: 651-663. - Link

  • VICKERS, J. 2016. The Problem of Induction. In: ZALTA, E. N. (ed.). Standford Encylopedia of Philosophy: Center for the Study of Language and Information (CSLI), Stanford University.

  • VINCENZO, C. 2016. Confirmation. In: ZALTA, E. N. (ed.) Standford Encyplodia of Philosophy. Center for the Study of Language and Information (CSLI), Stanford University.

  • WAGNER, Victoria E.; IGLEWSKI, Barbara H. P. aeruginosa biofilms in CF infection. Clinical reviews in allergy & immunology, 2008, 35.3: 124-134. - Link 

  • WENDT, H., HILLMER, A., REIMERS, K., KUHBIER, J. W., SCHAFER-NOLTE, F., ALLMELING, C., KASPER, C. & VOGT, P. M. 2011. Artificial skin--culturing of different skin cell lines for generating an artificial skin substitute on cross-weaved spider silk fibres. PLoS One, 6, e21833. - Link

  • JAMBUNATHAN, P. & ZHANG, K. C. 2016. Engineered biosynthesis of biodegradable polymers. Journal of Industrial Microbiology & Biotechnology, 43, 1037-1058. - Link 

  • WU, Y. L., WANG, H., QIU, Y. K., LIOW, S. S., LI, Z. & LOH, X. J. 2016. PHB-Based Gels as Delivery Agents of Chemotherapeutics for the Effective Shrinkage of Tumors. Adv Healthc Mater. - Link

  • Wu, Y.-L., Wang, H., Qiu, Y.-K., Liow, S.S., Li, Z. and Loh, X.J. (2016) ‘PHB-Based gels as delivery agents of Chemotherapeutics for the effective shrinkage of tumors’, Advanced Healthcare Materials, . doi: 10.1002/adhm.201600723. - Link 

  • YANG, T. H., KIM, T. W., KANG, H. O., LEE, S. H., LEE, E. J., LIM, S. C., OH, S. O., SONG, A. J., PARK, S. J. & LEE, S. Y. 2010. Biosynthesis of Polylactic Acid and Its Copolymers Using Evolved Propionate CoA Transferase and PHA Synthase. Biotechnology and Bioengineering, 105, 150-160. - Link 

  • Zacharof, M. P., & Lovitt, R. W. (2012). Bacteriocins Produced by Lactic Acid Bacteria A Review Article. APCBEE Procedia, 2(2), 50–56. - Link

  • ZENDO, T. (2013). Screening and Characterization of Novel Bacteriocins from Lactic Acid Bacteria. Bioscience, Biotechnology, and Biochemistry, 77(5), 893–899. - Link

  • Zendo, T., Yoneyama, F., & Sonomoto, K. (2010). Lactococcal membrane-permeabilizing antimicrobial peptides. - Link

  • BATTIN, M. P. 2003. Bioethics. In: WELLMAN, R. G. F. C. H. (ed.) A Companion to Applied Ethics. Blackwell Publishing Ltd.

  • CHRISTENSEN, A.-M. S. 2014. Dydsetik. In: CHRISTENSEN, A.-M. S. (ed.) Filosofisk Etik. Århus: Århus Universitetsforlag.

  • HEMPEL, C. G. 1966. Philosophy Of Natural Science. Foundations of Philosophy series. Princeton University.

  • HOLTUG, N. 2014. Konsekventialisme. In: CHRISTENSEN, A.-M. S. (ed.) Filosofisk Etik. Århus: Århus Universitetsforlag.

  • KAPPEL, K. 2011. Bioetik. In: CHRISTENSEN, A.-M. S. (ed.) Filosofisk Etik. Århus: Aarhus Universitetsforlag.

  • KLAUSEN, S. H. 2014. Hvad er videnskabsteori. Akademisk Forlag.

  • MARCHANT, G. E. & MOSSMAN, K. L. 2004. Arbitrary and capricious: The precautionary principle in the European Union courts, American Enterprise Institute.

  • NIELSEN, C. F. 2014. Deontologi In: CHRISTENSEN, A.-M. S. (ed.) Filosofisk Etik. Århus: Århus Universitetsforlag.

  • SCHURZ, G. 2013. Philosophy of science: A unified approach, Routledge.

  • STEEL, D. 2014. Philosophy and the precautionary principle, Cambridge University Press.

  • European Comission Directorate General Environment/Eurobarometer 295, attitudes of European Citizens towards the Environment, 2008 - (PDF-file)