Team:SDU-Denmark/Parts
Parts
This BioBrick contains the coding region of Phasin, a protein derived from R. eutropha. Phasin binds to and reduces the size of PHA granules, including PHB.
BioBrick encodes a short hemolysin tag. This tag can be attached to other proteins for secretion through the hemolysin pathway.
This BioBrick contains the native RBS for hemolysin B in R. eutropha. The brick also contains some native DNA prior to the RBS.
This BioBrick contains the coding region of hemolysin B. Hemolysin B is an ABC transporter, that secretes the toxin hemolysin.
This BioBrick contains the native RBS for hemolysin D in R. eutropha. The brick also contains some native DNA prior to the RBS.
This BioBrick contains the coding region for hemolysin D, part of the inner membrane complex of the hemolysin type II secretion pathway.
This BioBrick contains the sequence for the MaSp1 CD gene. This part is a monomer coding for the dragline spider silk MaSp1 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part K2018045, K2018046 and K2018047.
This BioBrick contains the sequence for the MaSp2 CD gene. This part is a monomer coding for the dragline spider silk MaSp2 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part K2018045, K2018046 and K2018047.
This part is a functional BioBrick containing the coding region of Laterosporulin, with promoter, RBS and terminator. Laterosporulin is a bacteriocin produced by Brevibacillus sp. strain, and exhibit a broad spectrum of antibacterial activity against bacterias like: B. subtilis , S. aureus, E. coli, P. aeruginosa, and L. monocytogenes.
This BioBrick contains the coding region of, ThuricinS, a bacteriocin produced by Bacillus thuringiensis. ThuricinS targets a broad spectrum of pathogens, including Pseudonoas aeruginosa and Enterobactoer Cloacae which is often found in correlation to burn and wound infection.
This BioBrick contains the coding region of LacticinQ, a bacteriocin produced by Lactococcus lactis QU5 and has shown bactericidal activity against Staphylococcus aureus Ma, Q., Yu, Z., Han, B., Wang, Q., & Zhang, R. (2012). Expression and Purification of Lacticin Q by Small Ubiquitin-Related Modifier Fusion in Escherichia coli. The Journal of Microbiology, 50(2), 326–331. http://doi.org/10.1007/s12275-012-1425-x.. It functions by forming large toroidal pores by distributing membrane lipid organization.
This BioBrick contains the coding region of Laterosporulin-ThuricinS, which is a hybrid bacteriocin with Laterosporulin and ThuricinS that we have designed.
This BioBrick contains the coding region of LacticinQ-LacticinZ, which is a hybrid bacteriocin with LacticinQ and LacticinZ that we have designed.
This BioBrick contains the coding region of PyocinS5, a bacteriocin produced by a specific strain of P. aeruginosa and elicit its effect against other strains of P. aeruginosa. Pyocin causes the cell membrane of target cells to be permeable and thereby causing leakage of intracellular materials, which cause cell death Ling, H., Saeidi, N., Haji Rasouliha, B., & Wook Chang, M. (2010). A predicted S-type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage. FEBS Letters, 584, 3354–3358. http://doi.org/10.1016/j.febslet.2010.06.021..
This BioBrick contains the coding region of our biofused phasin with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (K2018027) and hemolysin D (K2018029). This BioBrick will simply bid to PHA granules in the cytoplasm and reduce the size of these.
This part secretes PHB from PHB producing cells.
This part is phaCAB with a hybrid promoter combined with panK. It consists of K2018036 and K2018021.
This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of K2018036 and K2018030.
This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of K2018036, K2018030 and K2018021.
This part is phaCAB with a hybrid promoter. It consists of K880005 and K934001 .
This part is phaCAB with a hybrid promoter. It consists of K081005 and k934001.
This part is phaCAB with a hybrid promoter. It consists of K608004 and k934001.
This part is phaCAB with a hybrid promoter. It consists of K608003 and K934001.
This part is phaCAB with a hybrid promoter. It consists of J23104 and K934001.
This part is phaCAB with a hybrid promoter. It consists of J23106 and K934001.
We have created two part collections. The first one are our collection of bacteriocins, which all had been tested on different MRSA strains and P. aeruginosa
The following BioBricks are a part of the collection. K2018010, K2018011, K2018012, K2018014, K2018015, K2018019
We had also created a secretions system, which can secrete PHA, including PHB. The following BioBricks are a part of the collection. K2018022, K2018023, K2018026, K2018027, K2018028, K2018029, K2018049 and K2018050
This BioBrick contain standard iGEM primer VF2
This BioBrick contain standard iGEM primer VR
The part is J23104, a strong promoter.
The part is J23106, a strong promoter.
The part is composed of sub-parts J23100, a strong promoter and B0030, a strong RBS.
This part code the phaCAB with hybrid promoter. The BioBrick is created by Imperial College 2013
This part code the Staphylococcal pantothenate kinase II, that increases the synthesis of coenzyme A and thereby increases PHB yield.The BioBrick is created by Imperial College 2013
This BioBrick we got from the University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein, MaSp2. This part is called MaSp2 AB and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp2. The brick is compatible with initiator and part K1763003.
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called MaSp2 BC and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763002, K1763009 and K1763004.
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called MaSp2 CA and used in the ICA technique and is the third component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763003, K1763002 and K1763010.
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. A unique trade for this gene segment is that it codes for the same gene but is different because it contains a primer site. This part is called MaSp2 AB sequence core and used in the ICA technique and is an alternative first component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763003, K1763004, K1763012 and initiator.
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 AB and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763011, K1763012 and K1763004
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 BC and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763010 and K1763012
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 CA and used in the ICA technique and is the thrid component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763011, K1763010, K1763002, and K1763009
The part is composed of sub-parts J23104, a strong promoter and B0032, a medium RBS.
The part is composed of sub-parts J23104, a strong promoter and B0031, a weak RBS.
The part is composed of sub-parts J23100, a strong promoter and B0034, a strong RBS.
This part code the phaCAB metabolic pathway that synthesises PHB from acetyl-CoA. The BioBrick is created by Tokyo Tech 2012
