Team:TU-Eindhoven/Protocols

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✉

Protocols

• Description
• Background
• Scaffold Design
  • Heterodimer
  • Tetramer
• Experiments
  • Heterodimer
  • Tetramer
• Timeline
• Protocols

General Protocols

• Agarose Gel Electrophoresis
• Colony PCR
• Nanodrop
• PCR Purification
• General Necessities

Hover the desired protocol to learn more about it!

Cloning, Gibson Assembly & Mutagenesis

• Digestion
• Gibson Assembly
• Ligation
• Mutagenesis
• PCR Amplification
• Vector Linearization

Hover the desired protocol to learn more about it!

Transformation, Protein Expression & Purification

• MiniPrep
• Plating
• Protein Expression
• Protein Purification
• SDS_PAGE Analysis
• Small Culturing
• Transformation into E.Colo XL10-GOLD
• Transformation into NEB 5-Alpha Competent E. Coli
• Transformation into NovaBlue

Hover the desired protocol to learn more about it!

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