Team:UCAS/Part Collection
Best Basic Parts
In our project, we also tested 11 pairs of toxins and antitoxins, most of them are functional. It is estimated that bacteria harbors thousands of TA modules both on their genomes and plasmids. The actual number may be more than that. What is more, TA modules are commonly used in iGEM Competition to build biocontainment systems or ‘kill-switch’. So our work shall provide an abundant pool for future teams. Based on these, we believe these parts are competitive for Best Part Collection.
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K2150101 | Coding | TetX from Bacteroides fragilis | 1173 |
| BBa_K2150013 | Coding | fusion protein combining tetX with GFP | 1935 |
| BBa_K2150014 | Reporter | pTet + GFP | 937 |
| BBa_K2150015 | Reporter | pTet + TetX-GFP(fusion protein) | 2152 |
| BBa_K2150016 | Reporter | pT7 + GFP | 903 |
| BBa_K2150017 | Reporter | pT7 + TetX-GFP(fusion protein) | 2118 |
| BBa_K2150022 | Reporter | pTet + RBS + T7RNAP + Ter + RBS + pT7 + GFP + DT | 3701 |
| BBa_K2150028 | Reporter | pTet + RBS + T7RNAP + Ter + RBS + pT7 + tetX-GFP | 4916 |
| BBa_K2150031 | Regulatory | T7 promoter | 20 |
| BBa_K2150102 | Coding | toxin 136 (yobR) CDS | 744 |
| BBa_K2150103 | Coding | toxin 1204 CDS | 507 |
| BBa_K2150104 | Coding | toxin 6249 CDS | 319 |
| BBa_K2150105 | Coding | antitoxin 1204 CDS | 274 |
| BBa_K2150106 | Coding | antitoxin 6249 CDS | 218 |
| BBa_K2150110 | Device | LacI + Ptac + RiboJ + toxin 1204 | 2040 |
| BBa_K2150111 | Composite | LacI + Ptac + RiboJ + toxin 6249 | 1874 |
| BBa_K2150112 | Composite | TetR + Ptet + RiboJ + toxin 1204 | 669 |
| BBa_K2150113 | Composite | TetR + Ptet + RiboJ + toxin 6249 | 481 |
| BBa_K2150114 | Composite | TetR + Ptet + RiboJ + antitoxin 1204 | 436 |
| BBa_K2150115 | Composite | TetR + Ptet + RiboJ + antitoxin 6249 | 382 |
| BBa_K2150116 | Composite | LacI + Ptac + RiboJ + antitoxin 1204 | 1829 |
| BBa_K2150117 | Composite | LacI + Ptac + RiboJ + antitoxin 6249 | 1775 |
| BBa_K2150119 | Composite | LacI + Ptac + RiboJ + toxin 136 | 2299 |
| BBa_K2150120 | Composite | TetR + Ptet + RiboJ + toxin 136 | 906 |
| BBa_K2150121 | Coding | toxin 4222 CDS | 1320 |
| BBa_K2150122 | Coding | toxin 5980 CDS | 366 |
| BBa_K2150123 | Coding | toxin 5694 CDS | 357 |
