Team:UCAS/Parts

Parts

Overview

This summer, we constructed a number of parts, which include enzyme related parts and a collection of antitoxins and toxins. We also have 13 functional parts submitted.

Favorite Part

Among the parts we submitted, TetX is fully characterized and we believe it has the potential to compete for the Best Basic Part (See our wiki page: Best Basic Part).

Toxin ParE is sufficiently toxic and can be effectively neutralized by antitoxin ParD. This Mycobacterium tuberculosis-originated TA module is also orthogonal to the TA modules in our chassis organism. We believe this composite part is competitive enough for a Best Composite Part (See our wiki page: Best Composite Part).

Useful Pats

In our project, we also tested 11 pairs of toxins and antitoxins, most of them are functional. It is estimated that bacteria harbors thousands of TA modules both on their genomes and plasmids. The actual number may be more than that. What is more, TA modules are commonly used in iGEM Competition to build biocontainment systems or ‘kill-switch’. So our work shall provide an abundant pool for future teams. Based on these, we believe these parts are competitive for Best Part Collection (See our wiki page: Best Part Collection).

Part Table

Below, we listed all the parts we have documented this year:


Name Type Description Length
BBa_K2150101 Coding TetX from Bacteroides fragilis 1173
BBa_K2150013 Coding fusion protein combining tetX with GFP 1935
BBa_K2150014 Reporter pTet + GFP 937
BBa_K2150015 Reporter pTet + TetX-GFP(fusion protein) 2152
BBa_K2150016 Reporter pT7 + GFP 903
BBa_K2150017 Reporter pT7 + TetX-GFP(fusion protein) 2118
BBa_K2150022 Reporter pTet + RBS + T7RNAP + Ter + RBS + pT7 + GFP + DT 3701
BBa_K2150028 Reporter pTet + RBS + T7RNAP + Ter + RBS + pT7 + tetX-GFP 4916
BBa_K2150031 Regulatory T7 promoter 20
BBa_K2150102 Coding toxin 136 (yobR) CDS 744
BBa_K2150103 Coding toxin 1204 CDS 507
BBa_K2150104 Coding toxin 6249 CDS 319
BBa_K2150105 Coding antitoxin 1204 CDS 274
BBa_K2150106 Coding antitoxin 6249 CDS 218
BBa_K2150110 Device LacI + Ptac + RiboJ + toxin 1204 2040
BBa_K2150111 Composite LacI + Ptac + RiboJ + toxin 6249 1874
BBa_K2150112 Composite TetR + Ptet + RiboJ + toxin 1204 669
BBa_K2150113 Composite TetR + Ptet + RiboJ + toxin 6249 481
BBa_K2150114 Composite TetR + Ptet + RiboJ + antitoxin 1204 436
BBa_K2150115 Composite TetR + Ptet + RiboJ + antitoxin 6249 382
BBa_K2150116 Composite LacI + Ptac + RiboJ + antitoxin 1204 1829
BBa_K2150117 Composite LacI + Ptac + RiboJ + antitoxin 6249 1775
BBa_K2150119 Composite LacI + Ptac + RiboJ + toxin 136 2299
BBa_K2150120 Composite TetR + Ptet + RiboJ + toxin 136 906
BBa_K2150121 Coding toxin 4222 CDS 1320
BBa_K2150122 Coding toxin 5980 CDS 366
BBa_K2150123 Coding toxin 5694 CDS 357