• Non-pathogenic DH5 Alpha E. coli cells were used exclusively.
  • Nitrile gloves, lab coats, and safety glasses were used at all times during lab work.
  • Proper sterile laboratory technique was used at all times during lab work.
  • Antibiotics were carefully handled and only used when necessary.
  • The mutagen Ethidium Bromide (EtBr) was used frequently for gel electrophoresis and only in a designated area of the laboratory. Extreme care was used when preparing EtBr gels.
  • All biological and other waste was disposed of in the proper containers.