Team:UT-Knoxville/Protocols

UTK
iGEM

• HOME
• TEAM
• PROJECT
  • Motivation
  • Pseudomonas Putida
  • E. coli Platform
  • Substrates
  • Results
  • Indigo Production
  • Real-World Applications
  • Conclusion
• NOTEBOOK
  • Calendar
  • Methods
  • Protocols
• ATTRIBUTIONS
  • Sponsors
  • Group
• HUMAN PRACTICES
  • Engagement
  • Silver HP Requirement
• JUDGING
  • Medal Requirements
  • Collaborations
  • Submitted Parts

Protocols

ChemiComp Cell Prep and Heat Shock Transformation

Digestion

General PCR

Gibson Assembly

Ligation

Nanodrop

Phusion PCR