Difference between revisions of "Team:Arizona State/Experiments"
| Line 8: | Line 8: | ||
<h3> [1A] Polymerase Chain Reaction </h3> | <h3> [1A] Polymerase Chain Reaction </h3> | ||
| − | <p>PCR is used to </p> | + | <p>PCR is used to rapidly amplify certain segments of DNA that are selected via designed primers. This protocol was used to accomplish a variety of goals, from extraction of parts from a cloning vector to the addition of restriction cut sites at the ends of parts. </p> |
| − | + | <ol> | |
| + | <li>Defrost template, 10x Master Mix and primers | ||
| + | <li>Determine desired total working volume (~50uL) | ||
| + | </ol> | ||
| + | <p> | ||
<h5>What should this page contain?</h5> | <h5>What should this page contain?</h5> | ||
<ul> | <ul> | ||
Revision as of 00:20, 31 August 2016
Protocols
[1A] Polymerase Chain Reaction
PCR is used to rapidly amplify certain segments of DNA that are selected via designed primers. This protocol was used to accomplish a variety of goals, from extraction of parts from a cloning vector to the addition of restriction cut sites at the ends of parts.
- Defrost template, 10x Master Mix and primers
- Determine desired total working volume (~50uL)
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project
