Revision as of 14:01, 10 September 2016

Friday 26^th August

By Mathilde

Ligation products pPS16-003 and pPS16_004 frome the day before were purificated according to the usual protocol

By Mathilde

The Q5 PCR was conducted following the exact same protocol than the 25/08/2016 on the ligation product from the day before.

Migration Results

The two bands are at the expected size (1860 pb), so the fragment 2 (16-003 and pPS16_004 ligation product) is purified.

By Mathilde

The purification was made following the usual protocol. Each of those purification products were quantified on nanodrop.

Segment	DNA quantity (ng/µL)	260/230	260/280
1	84,20	1,25	1,84
2	169,88	1,29	1,83

@@ Line 10: / Line 10: @@
 Ligation products pPS16-003 and pPS16_004 frome the [[Team:Paris_Saclay/Notebook/August/25#Visualization|day before]] were purificated according to the usual [[Team:Paris_Saclay/Experiments#Purification_:_PCR_clean-up_with_NucleoSpin_Gel_and_PCR_Clean-up|protocol]]
 ====Q5 high fidelity PCR of pPS16_003 - pPS16_004 ligation product====
 '' By Mathilde ''
@@ Line 23: / Line 22: @@
 '' By Mathilde''
-The purification was made following the usual [[https://2016.igem.org/Team:Paris_Saclay/Experiments#Purification_:_PCR_clean-up_with_NucleoSpin_Gel_and_PCR_Clean-up|protocol]].
+The purification was made following the usual [[Team:Paris_Saclay/Experiments#Purification_:_PCR_clean-up_with_NucleoSpin_Gel_and_PCR_Clean-up|protocol]].
 Each of those purification products were quantified on nanodrop.
@@ Line 34: / Line 33: @@
 |-
 |1
-|2
-|-
 |84,20
 |1,25
 |1,84
 |-
+|2
 |169,88
 |1,29