The lab we used for our project this year is an level two disease lab associated with Dalhousie University and with the Nova Scotia Health Authority (NSHA). Because of this, we had many resources for maintaining our health and safety such as Dalhousie’s Biosafety Office, our supervisors Dr. Lois Murray, Dr. John Rohde, and Dr. Zhenyu Cheng, and the biosafety guidelines that pertain to NSHA labs.

In order to maintain our safety, we were consistently wearing personal protective equipment like lab coats, gloves closed-toes shoes and safety glasses when necessary.

Where it was applicable to do so, we used biological safety cabinets (BSC) with HEPA filters. The BSC - and also the laminar flow hoods – were used to protect our samples from contamination. All biohazardous consumable materials, such as pipette tips, serological pipette’s, agar plates and more, were place in biohazardous waste bags and disposed of according to NSHA policy. All contaminated liquids and reusable materials were autoclaved when appropriate and decontaminated with 70% ethanol or 10% bleach when autoclaving was not an option. Situations where autoclaving was not available includes decontaminating plastics and contaminated glass beads for examples. Lab surfaces were always decontaminated with 70% ethanol before and after use.

All the policies and procedures that were referenced for our biosafety concerns are contained below. This list contains documents from both Capital Health (now known as the Nova Scotia Health Authority) and Dalhousie University

• Biomedical Waste Management - NSHA
• Infection Prevention and Control Manual - Contact Procedures - NSHA
• Dalhousie University Biosafety Manual - Dalhousie University