Difference between revisions of "Team:Paris Saclay/Notebook/September/5"
MahnaziGEM (Talk | contribs) (Created page with "{{Team:Paris_Saclay/notebook_header}} = Monday 5<sup>th</sup> September= ==Lab work== ===Visualization=== {{Team:Paris_Saclay/notebook_footer}}") |
MahnaziGEM (Talk | contribs) |
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===Visualization=== | ===Visualization=== | ||
| + | ====fragments 1.1 with 1.2 Ligation AND 2.1 with 2.2 Ligation==== | ||
| + | ''By Mahnaz'' | ||
| + | |||
| + | Fragment 1.1 and 1.2 were ligated together as following | ||
| + | * 4µL of Fragment 1.1 PCR product from the 02/09/2016 | ||
| + | * 4µM of Fragment 1.2 PCR product from 02/09/2016 | ||
| + | * 1µL of Buffer T4 10X | ||
| + | * 1µL of ligase T4 enzyme | ||
| + | |||
| + | Fragment 2.1 and 2.2 were ligated together as following | ||
| + | * 4µL of Fragment 2.1 PCR product from the 02/09/2016 | ||
| + | * 4µM of Fragment 2.2 PCR product from 02/09/2016 | ||
| + | * 1µL of Buffer T4 10X | ||
| + | * 1µL of ligase T4 enzyme | ||
| + | |||
| + | The ligation product was put at room temperature for 1 hour. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 15:16, 21 September 2016
