(Created page with "{{Team:Paris_Saclay/notebook_header}} = Friday 23<sup>rd</sup> September= ==Lab work== ===Visualization=== ==== Glycerol stocks of clones X, X, X and X containing FRB - GFP...")
==== Glycerol stocks of clones X, X, X and X containing corrected dCas9 ST - GFP 11 in pSB1C3 (pPS16_017)====
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"By Maxence, Mahnaz & Coline"
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The glycerol stock of the bacteria with the following plasmids were made.
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*pPS16_017 (dCas9 ST - GFP 11) clone X
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*pPS16_017 (dCas9 ST - GFP 11) clone X
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*pPS16_017 (dCas9 ST - GFP 11) clone X
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*pPS16_017 (dCas9 ST - GFP 11) clone X
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500µL of liquid culture and 250 µL of glycerol 60% were mixed and put at -20°C.
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====Plasmids extraction of clones X, X, X and X containing corrected dCas9 ST - GFP 11 in pSB1C3 (pPS16_017)====
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"By Maxence, Mahnaz & Coline"
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The following plasmids were extracted using the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|"Charge Switch-Pro Plasmid Miniprep"]] kit:
