|FKBP amplification product from the 8th September<div id="FKBP amplification product from the 8th September"></div>
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|231.55
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|-
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|gblock 2.2 amplification product from the 8th September<div id="gblock 2.2 amplification product from the 8th September"></div>
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|183.9
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|-
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|pSB1C3 treated by DpnI from the 15th September<div id="pSB1C3 treated by DpnI from the 15th September"></div>
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|59.68
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|-
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|PCR product containing FKBP - GFP 10 from the 22nd September<div id="PCR product containing FKBP - GFP 10 from the 22nd"></div>
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|38.19
+
|-
+
|}
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+
====Gibson of cleaned up PCR products FKBP from 8th September x gblock 2.2 from the 8th September x pSB1C3 treated by DpnI====
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''By Maxence''
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Gibson was performed with cleaned up PCR products FKBP (insert 1) (obtained the 8th September) x gblock 2.2 (insert 2) (obtained the 8th September) x pSB1C3 treated by DpnI (plasmid) with the following protocol:
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+
For each 20μl of reaction, mix the following reagents :
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* 0.31 µL of insert 1
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* 0.2 µL of insert 2
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* 2 µL of plasmid
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* 7.49 µL of water
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* 10 µL of buffer mix
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Two controls were done: one without insert and one without buffer (water was added in order to have a total of 20 µL). The PCR was performed as follow : 1 hour at 50°C.
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====Transformation of DH5a cells with FKBP - GFP 10 in pSB1C3 (pPS16_018) obtained by Gibson====
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''By Maxence & Mahnaz''
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Dh5a cells were transformed with pSB1C3 containing FKBP - GFP 10 (pPS16_018), or controls (no buffer mix and plasmid alone) using the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]].
