Members' Attributions
All the data presented on this wiki was measured, collected, and analyzed by the team members. All the plasmids directly involved in the data presented on this wiki were constructed by the team members. The idea conceiving, content planning, execution, and result analysis of human practice were all done by the team members. The following is the detailed attribution:
Acknowledgement
We'd like to thank all those who have helped us over the summer, without whose sincere support The Uranium Reaper Project goals would have not been achieved.
General support
Prof. OUYANG Qi, Prof. LOU Chunbo, Dr. ZHANG Haoqian and other teachers helped us during our brainstorming and gave us useful suggestions.
Prof. OUYANG Qi and Prof. LIU Chunli kindly provided the laboratory to us for experiments.
Materials support
Dr. ZHANG Wenbing provided us with the plasmid of 3B(Tri-SpyCatcher within 15X linker).
We emailed Prof. ZHOU Lu for the Uranyl-binding Protein, also called SUP.
With Prof. LIU Chunli’s consent, we used his lab to do experiments on uranyl ion. The uranyl nitrate solution and Arsenazo III was provided by Prof. LIU.
Prof. LOU Chunbo provided us with the strain B.Subtilis and mSA, heavy metal ions binding proteins: lead-binding protein, mercury-binding protein and cadmium-binding protein.
Dr. DU Pei provided the beads to us and gave us experimental guidance.
We asked BIT-China for Interlab kit.
Experiment equipment support
Prof. OUYANG Qi lent the Akta Pure to us for all proteins purification.
Prof. CHANG Zengyi and Dr. WANG Qingsong provided us with Scanner and helped with the setup and scan of gels.
Prof. LIU Chunli provided us with Geiger counter to test the radiation level in and around our lab.
We thank Core Facilities, School of Life Sciences, Peking University, for assistance with ITC.
The State Key Laboratory of environmental simulation and pollution control provided the Aurora M90 mass spectrograph.
Theoretical guidance
The wiki designers of Peking iGEM 2015 ZHANG Yihao and WANG Yu gave us many suggestions about wiki building.
Dr. YU Daqi of Prof. OUYANG Qi’s lab helped us a lot in experimental operating and data analysis.
Prof. ZHANG Wenbing, Prof. LOU Chunbo and Dr. YU Daqi kindly helped our team with modeling of the project.
The instructors: Prof. OUYANG Qi, Prof. LOU Chunbo, Dr. ZHANG Haoqian and ZHANG Yihao coached us for the presentation.
Prof. LAI Luhua and Prof. RAO Yi participated in our presentation rehearsal and gave suggestions for revisions.
Synthetic biology course
From March to June, an introductory course of synthetic biology was hold by College of Life Sciences, Peking University.During the course, the teachers, also the instructors gave us kind guidance and judged our project design. In late June, we started in the lab to start our project.
