Difference between revisions of "Team:Freiburg/Notebook"
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<span style="color:#e8a126">I) Cloning</span> <br><br> | <span style="color:#e8a126">I) Cloning</span> <br><br> | ||
Revision as of 09:33, 4 October 2016
Our Protocols
I) Cloning
To keep an overview of the cloned constructs every plasmid was assigned to an ID: pIG16_000. All used oligos were assigned to an ID as well: oIG16_000. The complete list of the resulting bacterial strains and oligos can be found in the attached Excel files. The spore coat proteins cotZ, cotG, cotB and cgeA were amplified from the genome of B. subtilis 168. The anti-GFP nanobody and the GST were amplified from plasmids provided by Dr. Nicole Gensch and Dr. Maximilian Ulbrich. For the cloning strategy see Project - Approach
II) Bacillus subtilisTo keep an overview of the cloned constructs every plasmid was assigned to an ID: pIG16_000. All used oligos were assigned to an ID as well: oIG16_000. The complete list of the resulting bacterial strains and oligos can be found in the attached Excel files. The spore coat proteins cotZ, cotG, cotB and cgeA were amplified from the genome of B. subtilis 168. The anti-GFP nanobody and the GST were amplified from plasmids provided by Dr. Nicole Gensch and Dr. Maximilian Ulbrich. For the cloning strategy see Project - Approach
III)Expression analysis
IV) Targeting
V) Delivery
