Difference between revisions of "Team:SCAU-China/Collaborations"

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{{SCAU-China}}
 
{{SCAU-China}}
 
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<div class="column full_size judges-will-not-evaluate">
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<h3>★ ALERT! </h3>
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@charset "utf-8";
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">team collaboration silver medal criterion</a>. </p>
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/* CSS Document */
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body,h1,h2,h3,h4,h5,h6,dl,dt,dd,ul,ol,li,th,td,p,blockquote,pre,form,fieldset,legend,input,button,textarea,hr{margin:0;padding:0}
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Evaluated_Pages/Instructions"> Instructions for Evaluated Pages </a>.</p>
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#page{
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width:1200px;
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margin:0 auto;
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background:#fcfafb;
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}
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#header{
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height:780px;
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position:relative;
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background:url(./images/header-img.jpg) no-repeat center bottom;
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}
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#header .menu{
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height:106px;
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text-align:right;
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background:rgba(0,0,0,0.2)
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#header .menu li{
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line-height:106px;
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padding:0 12px;
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bottom:12px;
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#header .triangle .click{
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margin:0 auto;
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cursor:pointer;
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background:url(../images/triangle.png) no-repeat;
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}
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#content .vecter .nav{
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height:70px;
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display:block;
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line-height:70px;
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text-align:center;
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background:#d5d5d5;
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}
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#content .vecter .nav li{
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display:inline-block;
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text-align:center;
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padding:0 20px;
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#content .vecter .nav li a{
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font-size:20px;
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font-weight:bold;
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</style>
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</head>
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<body>
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<div id="page">
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  <div id="header">
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  <div class="menu">
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  <ul>
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<li><a href="#">Home</a></li>
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<li><a href="#">Team</a></li>
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<li><a href="#">Project</a></li>
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<li><a href="#">Result</a></li>
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<li><a href="#">Notebook</a></li>
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<li><a href="#">HP</a></li>
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<li><a href="#">Judging</a></li>
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</ul>
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  </div>
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  <div class="triangle"><a class="click"></a></div>
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  </div>
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  <div id="content">
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    <div class="vecter">
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      <ul class="nav">
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      <a id="vecter"></a>
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      <li><a href="#vecter" class="on">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="images/img_1.jpg"></div>
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      <div class="text">Fig.1 Not I digestion of constructs cotaining different numbers of genes.
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Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.
 
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    <div class="vecter">
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      <ul class="nav">
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<div class="column full_size">
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      <a id="tissue"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue" class="on">Tissue Culture</a></li>
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<p>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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</p>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <p><img src="images/img_2.jpg"></p>
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      <p><img src="images/img_3.jpg"></p>
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      <p><img src="images/img_4.jpg"></p>
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      </div>     
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="pcr"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr" class="on">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <p><img src="images/img_5.jpg"></p>
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      <p><img src="images/img_6.jpg"></p>
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      </div>     
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="phenotype"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype" class="on">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <p><img src="images/image014.jpg">&nbsp;&nbsp;<img src="images/image016.png">&nbsp;&nbsp;<img src="images/image015.png"></p><br>
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      <p><img src="images/image020.jpg">&nbsp;&nbsp;<img src="images/image022.png">&nbsp;&nbsp;<img src="images/image018.png"></p>
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      </div>     
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="hplc"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc" class="on">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="images/image024.png"></div>
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      <div class="text">Fig.n HPLC analysis diagram of standard samples of astaxanthin(a);
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HPLC analysis diagram of  samples extract  from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of  samples extract  from  HG-WT rice (c);<br><br>
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Compared with the standard samples with HPLC analysis diagram, you can see the sample extract  from rice and standard samples  have the same peak at the same time, show that the  transgenic rice seed which carry the four genes  expressed astaxanthin. Results indicate  that we are successful synthesize astaxanthin by rice endosperm synthetic .</div> 
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<div class="img"><img src="images/image026.png"></div>
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<div class="img"><img src="images/image028.png"></div>
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<div class="img"><img src="images/image030.png"></div>   
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="modeling"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling" class="on">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="images/image032.png"></div>
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      <div class="text">Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .</div> 
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    </div>
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<h4> Which other teams can we work with? </h4>
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
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In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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<p>
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Here are some suggestions for projects you could work on with other teams:
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</p>
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<ul>
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<li> Improve the function of another team's BioBrick Part or Device</li>
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<li> Characterize another team's part </li>
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<li> Debug a construct </li>
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<li> Model or simulating another team's system </li>
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<li> Test another team's software</li>
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<li> Help build and test another team's hardware project</li>
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<li> Mentor a high-school team</li>
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</ul>
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Revision as of 12:06, 4 October 2016

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Fig.1 Not I digestion of constructs cotaining different numbers of genes. Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.

    


    

Fig.n HPLC analysis diagram of standard samples of astaxanthin(a); HPLC analysis diagram of samples extract from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of samples extract from HG-WT rice (c);

Compared with the standard samples with HPLC analysis diagram, you can see the sample extract from rice and standard samples have the same peak at the same time, show that the transgenic rice seed which carry the four genes expressed astaxanthin. Results indicate that we are successful synthesize astaxanthin by rice endosperm synthetic .
Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .