Difference between revisions of "Team:NKU China/Safety"

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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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        }       
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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        $(function () {
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<li>Choosing parts that will not harm humans / animals / plants</li>
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            //particles();
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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              }
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          },
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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    <div class="main" id="safety">
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        <div class="h1">Safety</div>
 +
        <div class="p">Safety has always been an important concern in our laboratory, both in project design and operations during experiment process. This year we used two kinds of <i>Escherichia coli</i> (DH5&alpha; in part construction and MG1655 in project). According to the official <a href="https://2016.igem.org/Safety/Risk_Groups">iGEM guidelines of Risk Groups</a>, <i>E. coli</i> is categorized as a Risk Group 1 organism that "do not cause disease in healthy adult humans" when used properly. Also, The risk group level of <i>E. coli</i> is correspondent with our laboratory safety level (Safety Level 1). To make sure that everything is safe enough, we have seriously managed Individual Safety, Environmental Safety, Biological Safety based on project as well as Safe Shipping.</div>
 +
        <section>
 +
            <div class="h2">Individual Safety</div>
 +
            <ol>
 +
                <li>All members participating in the iGEM project this year have taken safety training courses given by the department experiment center that we acknowledged general safety managements such as fire-fighting strategies, disposition of biochemical materials, proper usages of microwave oven, laminar flow bench and other apparatus.</li>
 +
                <li>All members have carefully read <a href="https://2016.igem.org/Safety/What_is_Safety#Project_Design">Safe Project Design</a>, <a href="https://2016.igem.org/Safety/What_is_Safety#Lab_Work">Safe Lab Work</a>, <a href="https://2016.igem.org/Safety/What_is_Safety#Shipment">Safe Shipment</a> and other requirements proposed by <a href="https://2016.igem.org/Safety">iGEM Safety Committee</a>. We filled up the <a href="https://2016.igem.org/Safety/Final_Safety_Form">Final Safety Form</a> according to the real situation in our laboratory.</li>
 +
                <li>Nitrile gloves, masks, closed shoes were strictly required in laboratory to prevent potential threats of personal health.</li>
 +
                <li>Resting area and Experiment area were clearly separated to confine eating and drinking strictly in resting area.</li>
 +
            </ol>
 +
        </section>
 +
        <section>
 +
            <div class="h2">Environmental Safety</div>
 +
            <!--<img src="https://static.igem.org/mediawiki/2016/f/f2/T--NKU_China--9.jpg" style="width:20vw;">-->
 +
            <ol>
 +
                <li>Biological materials were not allowed to be taken outside the laboratory without sterilization. All of the used materials (bacterial samples, plasmids, reagents, agar, etc.) after experiments were sterilized before leaving the laboratory and collected altogether for further management.</li>
 +
                <li>Regular cleaning of the laboratory trash was arranged every day to maintain a clean and tidy laboratory environment.</li>
 +
            </ol>   
 +
        </section>
 +
        <section>
 +
            <div class="h2">Biological Safety Concerns based on project</div>
 +
            <div class="p">With the advent of synthetic biology, genetically modified microorganisms are being increasingly used for biomedical, industrial and environmental applications. Deployment of these engineered microbes in large scales and open environments calls for the development of safe and secure means to restrain their proliferation.</div>
 +
            <div class="p">This summer, we added <i>mf-lon</i> <i>ssrA</i> tag into 5 essential genes using CRIPSR/Cas9 method. By applying gene circuits to control mf-lon protease expression under the assigned biocontainment conditions, we can block essential gene expression to kill the cell upon loss of the biocontainment signal.</div>
 +
        </section>
 +
        <section>
 +
            <div class="h2">Safe Shipping</div>
 +
            <div class="p">We sent our BioBricks through the standard shipping process required by iGEM headquarter.</div>
 +
        </section>
 +
    </div>
 +
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Revision as of 07:35, 6 October 2016

Safety
Safety has always been an important concern in our laboratory, both in project design and operations during experiment process. This year we used two kinds of Escherichia coli (DH5α in part construction and MG1655 in project). According to the official iGEM guidelines of Risk Groups, E. coli is categorized as a Risk Group 1 organism that "do not cause disease in healthy adult humans" when used properly. Also, The risk group level of E. coli is correspondent with our laboratory safety level (Safety Level 1). To make sure that everything is safe enough, we have seriously managed Individual Safety, Environmental Safety, Biological Safety based on project as well as Safe Shipping.
Individual Safety
  1. All members participating in the iGEM project this year have taken safety training courses given by the department experiment center that we acknowledged general safety managements such as fire-fighting strategies, disposition of biochemical materials, proper usages of microwave oven, laminar flow bench and other apparatus.
  2. All members have carefully read Safe Project Design, Safe Lab Work, Safe Shipment and other requirements proposed by iGEM Safety Committee. We filled up the Final Safety Form according to the real situation in our laboratory.
  3. Nitrile gloves, masks, closed shoes were strictly required in laboratory to prevent potential threats of personal health.
  4. Resting area and Experiment area were clearly separated to confine eating and drinking strictly in resting area.
Environmental Safety
  1. Biological materials were not allowed to be taken outside the laboratory without sterilization. All of the used materials (bacterial samples, plasmids, reagents, agar, etc.) after experiments were sterilized before leaving the laboratory and collected altogether for further management.
  2. Regular cleaning of the laboratory trash was arranged every day to maintain a clean and tidy laboratory environment.
Biological Safety Concerns based on project
With the advent of synthetic biology, genetically modified microorganisms are being increasingly used for biomedical, industrial and environmental applications. Deployment of these engineered microbes in large scales and open environments calls for the development of safe and secure means to restrain their proliferation.
This summer, we added mf-lon ssrA tag into 5 essential genes using CRIPSR/Cas9 method. By applying gene circuits to control mf-lon protease expression under the assigned biocontainment conditions, we can block essential gene expression to kill the cell upon loss of the biocontainment signal.
Safe Shipping
We sent our BioBricks through the standard shipping process required by iGEM headquarter.