Difference between revisions of "Team:Toulouse France/Collaborations"

Line 99: Line 99:
 
<div class="textContent">
 
<div class="textContent">
 
<div class="column-left">
 
<div class="column-left">
 +
<h2>Tutorials</h2>
 
 
−
<div id="teamGallery">
+
<div id="teamGallery2">
−
<h2>Tutorials</h2>
+
<h2>Digestion</h2>
 
 
 
<div class="box">
 
<div class="box">
Line 111: Line 112:
 
</div>
 
</div>
 
 
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Leiden1">
 +
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden1">
 +
</a>
 +
</div>
 +
</div>
 +
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Portugal1">
 +
<img src="https://static.igem.org/mediawiki/2016/2/2a/Toulouse_France_Collaboration_Portugalflag.png" title="Portugal1">
 +
</a>
 +
</div>
 +
</div>
 +
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Espagne1">
 +
<img src="https://static.igem.org/mediawiki/2016/f/f3/Toulouse_France_Collaboration_Mexiqueflag.png" title="Espagne1">
 +
</a>
 +
</div>
 +
</div>
 +
 +
</div>
 +
<div style="clear: both"></div>
 +
 +
<div id="teamGallery2">
 +
<h2>Ligation</h2>
 +
 
<div class="box">
 
<div class="box">
 
<div class="innerBox">
 
<div class="innerBox">
Line 117: Line 148:
 
</a>
 
</a>
 
</div>
 
</div>
−
</div>
+
</div>
 
 
 
<div class="box">
 
<div class="box">
 
<div class="innerBox">
 
<div class="innerBox">
−
<a href="https://2016.igem.org/Team:Toulouse_France/Team#ImperialCollege3">
+
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Leiden2">
−
<img src="https://static.igem.org/mediawiki/2016/9/98/Toulouse_France_Collaboration_GBflag.png" title="Imperial College3">
+
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden2">
 
</a>
 
</a>
 
</div>
 
</div>
Line 129: Line 160:
 
<div class="box">
 
<div class="box">
 
<div class="innerBox">
 
<div class="innerBox">
−
<a href="https://2016.igem.org/Team:Toulouse_France/Team#ImperialCollege4">
+
<a href="https://2016.igem.org/Team:Toulouse_France/Team#Portugal2">
−
<img src="https://static.igem.org/mediawiki/2016/9/98/Toulouse_France_Collaboration_GBflag.png" title="Imperial College4">
+
<img src="https://static.igem.org/mediawiki/2016/2/2a/Toulouse_France_Collaboration_Portugalflag.png" title="Portugal2">
 +
</a>
 +
</div>
 +
</div>
 +
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Espagne2">
 +
<img src="https://static.igem.org/mediawiki/2016/f/f3/Toulouse_France_Collaboration_Mexiqueflag.png" title="Espagne2">
 
</a>
 
</a>
 
</div>
 
</div>
 
</div>
 
</div>
 +
 +
 +
</div>
 +
<div style="clear: both"></div>
 +
 +
<div id="teamGallery2">
 +
<h2>PCR</h2>
 
 
−
 
 
<div class="box">
 
<div class="box">
 
<div class="innerBox">
 
<div class="innerBox">
−
<a>
+
<a href="https://2016.igem.org/Team:Toulouse_France/Team#ImperialCollege3">
−
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden1">
+
<img src="https://static.igem.org/mediawiki/2016/9/98/Toulouse_France_Collaboration_GBflag.png" title="Imperial College3">
 +
</a>
 +
</div>
 +
</div>
 +
 
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Leiden3">
 +
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden3">
 
</a>
 
</a>
 
</div>
 
</div>
Line 146: Line 199:
 
<div class="box">
 
<div class="box">
 
<div class="innerBox">
 
<div class="innerBox">
−
<a>
+
<a href="https://2016.igem.org/Team:Toulouse_France/Team#Portugal3">
−
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden2">
+
<img src="https://static.igem.org/mediawiki/2016/2/2a/Toulouse_France_Collaboration_Portugalflag.png" title="Portugal3">
 
</a>
 
</a>
 
</div>
 
</div>
 
</div>
 
</div>
 +
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Espagne3">
 +
<img src="https://static.igem.org/mediawiki/2016/f/f3/Toulouse_France_Collaboration_Mexiqueflag.png" title="Espagne3">
 +
</a>
 +
</div>
 +
</div>
 +
 +
</div>
 +
<div style="clear: both"></div>
 +
 +
<div id="teamGallery2">
 +
<h2>Transformation</h2>
 
 
 
<div class="box">
 
<div class="box">
 
<div class="innerBox">
 
<div class="innerBox">
−
<a>
+
<a href="https://2016.igem.org/Team:Toulouse_France/Team#ImperialCollege4">
−
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden3">
+
<img src="https://static.igem.org/mediawiki/2016/9/98/Toulouse_France_Collaboration_GBflag.png" title="Imperial College4">
 
</a>
 
</a>
 
</div>
 
</div>
 
</div>
 
</div>
 +
 
 
 
<div class="box">
 
<div class="box">
 
<div class="innerBox">
 
<div class="innerBox">
−
<a>
+
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Leiden4">
 
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden4">
 
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden4">
 
</a>
 
</a>
 
</div>
 
</div>
 
</div>
 
</div>
−
+
 
−
</div>
+
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Team#Portugal44">
 +
<img src="https://static.igem.org/mediawiki/2016/2/2a/Toulouse_France_Collaboration_Portugalflag.png" title="Portugal4">
 +
</a>
 +
</div>
 +
</div>
 +
 
 +
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Espagne4">
 +
<img src="https://static.igem.org/mediawiki/2016/f/f3/Toulouse_France_Collaboration_Mexiqueflag.png" title="Espagne4">
 +
</a>
 +
</div>
 +
</div>
 +
</div>
 
<div style="clear: both"></div>
 
<div style="clear: both"></div>
  
Line 176: Line 260:
 
<div class="column-right">
 
<div class="column-right">
  
−
<div class="descriptionArea" id="empty"></div>
+
<div class="descriptionArea2" id="empty"></div>
  
−
<div  class="descriptionArea" id="ImperialCollege1">
+
<div  class="descriptionArea2" id="ImperialCollege1">
 
 
 
<div id=video style="margin-left:80px">  
 
<div id=video style="margin-left:80px">  
Line 193: Line 277:
 
</div>
 
</div>
  
−
<div class="descriptionArea" id="Imperial College2">
+
<div class="descriptionArea2" id="Imperial College2">
−
<img src="https://static.igem.org/mediawiki/2016/1/18/Toulouse_France_Soukaina2.jpg" style="height:500px; width:auto;">
+
<div id=video style="margin-left:80px">
−
<h3>Soukaïna Timouma</h3>
+
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/4/47/Toulouse_France_TutoLigation_english.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 +
<h3>tuto ligation</h3>
 
<p>
 
<p>
  
Line 201: Line 291:
 
</div>
 
</div>
  
−
<div class="descriptionArea" id="Imperial College3">
+
<div class="descriptionArea2" id="Imperial College3">
−
<img src="https://static.igem.org/mediawiki/2016/3/37/Toulouse_France_Fanny2.jpg" style="height:500px; width:auto;">
+
<div id=video style="margin-left:80px">
−
<h3>Fanny Leclerc</h3>
+
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/e/e9/Toulouse_France_TutoPCR_english.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 +
<h3>c</h3>
 
<p>
 
<p>
 
</p>
 
</p>
 
</div>
 
</div>
 
 
−
<div class="descriptionArea" id="Imperial College4">
+
<div class="descriptionArea2" id="Imperial College4">
−
<img src="https://static.igem.org/mediawiki/2016/0/0c/Toulouse_France_Marine2.jpg">
+
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/4/49/Toulouse_France_TutoTransformation_english.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
<h3>Marine Dubois</h3>
 
<h3>Marine Dubois</h3>
 
<p>
 
<p>
−
 
 
</p>
 
</p>
 
</div>
 
</div>
 
 
−
<div class="descriptionArea" id="Leiden1">
+
<div class="descriptionArea2" id="Leiden1">
−
<img src="https://static.igem.org/mediawiki/2016/e/e5/Toulouse_France_Anissa2.jpg" style="height:500px; width:auto;">
+
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/3/38/Toulouse_France_TutoDigestion_dutch.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
<h3>Anissa Dieudonné</h3>
 
<h3>Anissa Dieudonné</h3>
 
<p>
 
<p>
Line 224: Line 331:
 
</div>
 
</div>
 
 
−
<div class="descriptionArea" id="Leiden2">
+
<div class="descriptionArea2" id="Leiden2">
−
<img src="https://static.igem.org/mediawiki/2016/7/73/Toulouse_France_Camille3.jpg">
+
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/d/d6/Toulouse_France_TutoLigation_dutch.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
<h3>Camille Roux</h3>
 
<h3>Camille Roux</h3>
 
<p>
 
<p>
Line 231: Line 344:
 
</div>
 
</div>
 
 
−
<div class="descriptionArea" id="Leiden3">
+
<div class="descriptionArea2" id="Leiden3">
−
<img src="https://static.igem.org/mediawiki/2016/2/29/Toulouse_France_Manon2.jpg" style="height:500px; width:auto;">
+
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/7/70/Toulouse_France_TutoPCR_dutch.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
 
<h3>Manon Barthe</h3>
 
<h3>Manon Barthe</h3>
 
<p>
 
<p>
Line 239: Line 358:
 
</div>
 
</div>
 
 
−
<div class="descriptionArea" id="Leiden4">
+
<div class="descriptionArea2" id="Leiden4">
−
<img src="https://static.igem.org/mediawiki/2016/2/2f/Toulouse_France_Oumnia2.jpg" style="height:500px; width:auto;">
+
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/f/f2/Toulouse_France_TutoTransformation_dutch.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
 
<h3>Oumnia Karim</h3>
 
<h3>Oumnia Karim</h3>
 
<p>
 
<p>
Line 246: Line 371:
 
</p>
 
</p>
 
</div>
 
</div>
−
+
 
 +
<div  class="descriptionArea2" id="Portugal1">
 +
 +
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/7/75/Toulouse_France_TutoDigestion_portu.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
 +
<h3>Imperial College of London team</h3>
 +
 +
<p> Tuto digestion
 +
</p>
 +
</div>
 +
 
 +
<div class="descriptionArea2" id="Portugal2">
 +
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/7/71/Toulouse_France_TutoLigation_portu.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 +
<h3>Soukaïna Timouma</h3>
 +
<p>
 +
 
 +
</p>
 +
</div>
 +
 
 +
<div class="descriptionArea2" id="Portugal3">
 +
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/6/6b/Toulouse_France_TutoPCR_portu.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
 +
<h3>Fanny Leclerc</h3>
 +
<p>
 +
</p>
 +
</div>
 +
 +
<div class="descriptionArea2" id="Portugal4">
 +
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/3/37/Toulouse_France_TutoTransformation_portu.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
 +
<h3>Marine Dubois</h3>
 +
<p>
 +
</p>
 +
</div>
 +
 +
<div class="descriptionArea2" id="Espagne1">
 +
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/d/d1/Toulouse_France_TutoDigestion_span.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
 +
<h3>Anissa Dieudonné</h3>
 +
<p>
 +
 
 +
</p>
 +
</div>
 +
 +
<div class="descriptionArea2" id="Espagne2">
 +
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/a/a0/Toulouse_France_TutoLigation_span.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
 +
<h3>Camille Roux</h3>
 +
<p>
 +
</p>
 +
</div>
 +
 +
<div class="descriptionArea2" id="Espagne3">
 +
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
 +
<h3>Manon Barthe</h3>
 +
<p>
 +
 +
</p>
 +
</div>
 +
 +
<div class="descriptionArea2" id="Espagne4">
 +
<div id=video style="margin-left:80px">
 +
<video width="800" height="450" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/d/d1/Toulouse_France_TutoTransformation_span.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
</div>
 +
 
 +
<h3>Oumnia Karim</h3>
 +
<p>
 +
 
 +
</p>
 +
</div>
 
</div>
 
</div>
 
 

Revision as of 18:25, 7 October 2016

iGEM Toulouse 2016

Collaborations



Protocol videos
Our team decided to create protocol videos in order to help young scientists in manipulation. During our experiments, we filmed 4 steps of cloning: PCR, digestion, ligation and transformation. We asked other teams to translate protocols in their mother tongue. We obtained protocol videos in English with the Imperial College London team, in Spanish thanks to the TEC-Monterrey team, in Dutch with Leiden team, in Chinese with BIT-China team, in Portuguese thanks to EEL-USP team and in French with the collaboration of the Bordeaux team.
We wrote the entire script and we added some guidance in red (minutes for each paragraphs for instance). Teams had to translate and record the script. Now, we possess a lot of protocols explained in six different languages, accessible to everybody on Youtube and on our wiki.

Growth study of B. subtilis and P. fluorescens
In exchange for recording the script in English, we studied the optimal growth of Bacillus subtilis and Pseudomonas fluorescens for the Imperial London College team. First, we did an experimental plan to observe if pH is in interaction with temperature. Finally, the conclusion was that pH and temperature were two independent parameters. We ran the experiments again, changing only one parameter.


Results

Optimum Temperature (°C)

Optimum pH

Doubling time (minutes)

Bacillus subtilis (WT 168)

34-37

8

41

Pseudomonas fluorescens (SBW25)

<29

7,5

<80


Optimum temperatures and pH and the doubling times are summarized in the table for our two strains.

3D conception
Bordeaux

Receipt of plasmids
We want to thank the 2012 Munich team for sending us 4 different shuttle plasmids. One of these, pBs0k-p, was very useful because we used it for cloning in E.coli and Bacillus subtilis.

Mailing of kit
2016 Evry team asked us to send a Ludox solution that was in our iGEM Kit. They stored their own solution at -20°C so it was not usable anymore. But it was important for them to participate to the InterLab project so we helped them.

Protocol translation
2016 METU HS Ankara team wanted to create a database, which consists of different language protocols. They asked us to translate their protocol in French.

Surveys
We filled in different surveys all along the year. Among them, surveys for:
    Bdx
    IONIS
    Evry
    Valencia
    TecCEM Collegiate
    Purdue
    UBGM

Tutorials

Digestion

Ligation

Transformation

Imperial College of London team

Tuto digestion

tuto ligation

c

Marine Dubois

Anissa Dieudonné

Camille Roux

Manon Barthe

Oumnia Karim

Imperial College of London team

Tuto digestion

Soukaïna Timouma

Fanny Leclerc

Marine Dubois

Anissa Dieudonné

Camille Roux

Manon Barthe

Oumnia Karim


Contacts
    🏠 INSA Toulouse
    135, Avenue de Rangueil
    31077 TOULOUSE Cedex 4
    France
  • ☎ +00 (33) 6 13 64 89 40
  • ✉ igemtoulouse2016@gmail.com